Chemical analyses and anthelmintic effects of Artemisia campestris essential oil

Abidi, Anouar; Sebai, Essia; Dhibi, Mokhtar; Alimi, Dhouha; Rekik, Mourad; B’chir, Fatma; Maizels, Rick M.; Akkari, Hafidh

doi:10.1016/j.vetpar.2018.10.003

Cited by 28 publications

(17 citation statements)

References 37 publications

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“…The effects of the anthelmintic assays were dose dependent and the best activity was more visible at the highest dose of 5 000 mg/kg by day 7 post-treatment, and resulted in a 76.33% worm burden reduction and a 74% reduction in the faecal egg count. Similar findings of the dose-dependent anthelmintic effects of the A. campestris essential oil against H. polygyrus have also been reported in our laboratory (Abidi et al 2018). Satrija et al (1995) tested the anthelmintic activity of Papaya latex in mice infected with H. polygyrus and found a high reduction in the worm counts at the highest dose (8 g/kg).…”

Section: Discussionsupporting

confidence: 86%

“…The anthelmintic activity of essential oils from various Tunisian plants against H. contortus has previously been reported. The maximum inhibition effectiveness rates of the Artemisia campestris essential oil on the egg hatching and adult motility were 100% and 66.6% at 2 mg/ml and 0.5 mg/ml, (Abidi et al 2018). The essential oil of Ruta chalepensis inhibited 100% of the H. contortus egg hatching at 1 mg/ml and 87.5 % of the adult worm motility at 1 mg/ml (Akkari et al 2015).…”

Section: Discussionmentioning

confidence: 96%

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Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Abidi¹,

Sebai²,

Dhibi³

et al. 2020

Vet. Med.

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Because of the development of resistance in helminths against major anthelmintic drugs, the search for alternatives is necessary. Medicinal plants are being studied as an alternative source of anthelmintics against gastrointestinal nematodes. The objective of this study is to analyse the chemical composition and evaluate the anthelmintic efficacy of Origanum majorana essential oil. The determination of the chemical composition by gas chromatography/mass chromatography (GC/MS) revealed that the essential oil was dominated by terpenoids, particularly carvacrol (35.65%) and terpenic hydrocarbons p-cymene (15.82%). The in vitro anthelmintic effects against Haemonchus contortus were assessed by an egg hatch assay (EHA) and an adult worm motility assay (AWMA) compared with the reference drug albendazole. The essential oil showed ovicidal activity at all the tested concentrations (1, 2, 4 and 8 mg/ml) and more than 80% egg hatching inhibition was observed at the highest dose (8 mg/ml). Exposure to 0.5 mg/ml of the essential oil for eight hours induced a 50% inhibition in the worm motility. The in vivo study was performed on H. polygyrus by measuring the egg count reduction (ECR) and adult worm count reduction (AWCR) following the treatment of the animals with different doses (2 000, 4 000 and 5 000 mg/kg) of the plant essential oil, and 22 mg/kg of albendazole as the positive control. The results showed that 5 000 mg/kg of the essential oil inhibited the egg count and adult worm count by 76.3 and 74.0%, respectively, seven days post treatment. These findings support the possible use of O. majorana essential oil to control gastrointestinal nematodes.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 96%

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Abidi¹,

Sebai²,

Dhibi³

et al. 2020

Vet. Med.

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“…We did not determine the in vivo toxicity of the tested oils or investigate their effect on ruminal microbiota. However, oral administration of essential oil components, such as linalool, geraniol and geranyl acetate did not alter the hematological parameters, body weight or tissue of treated compared to control rodents [14,31]. Some essential oils and their components, especially terpenes have been shown to have antibacterial activity and can alter bacterial growth including ruminal microflora [51].…”

Section: Discussionmentioning

confidence: 99%

“…Various studies have demonstrated the anthelmintic activity of some essential oils and their main components against GINs especially H. contortus [10,21,[31][32][33][34]. In the present study, we evaluated the activity of a coriander essential oil whose main components were linalool (68.03%), α-tinene (9.71%), γ-terpinene (10.48%) and camphor (11.76%) ( Figure S2), and five common essential oil components, namely geraniol, geranyl acetate, eugenol, methyl iso-eugenol, and linalool, against the motility of L3s of H. contortus, T. axei, T. colubriformis, T. vitrinus, T. circumcincta and C. oncophora.…”

Section: Discussionmentioning

confidence: 99%

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

et al. 2020

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The anthelmintic effects of extracted coriander oil and five pure essential oil constituents (geraniol, geranyl acetate, eugenol, methyl iso-eugenol, and linalool) were tested, using larval motility assay, on the third-stage larvae (L3s) of Haemonchus contortus, Trichostrongylus axei, Teladorsagia circumcincta, Trichostrongylus colubriformis, Trichostrongylus vitrinus and Cooperia oncophora. Coriander oil and linalool, a major component of tested coriander oil, showed a strong inhibitory efficacy against all species, except C. oncophora with a half maximal inhibitory concentration (IC50) that ranged from 0.56 to 1.41% for the coriander oil and 0.51 to 1.76% for linalool. The coriander oil and linalool combinations conferred a synergistic anthelmintic effect (combination index [CI] <1) on larval motility comparable to positive control (20 mg/mL levamisole) within 24 h (p < 0.05), reduced IC50 values to 0.11–0.49% and induced a considerable structural damage to L3s. Results of the combined treatment were validated by quantitative fluorometric microplate-based assays using Sytox green, propidium iodide and C12-resazurin, which successfully discriminated live/dead larvae. Only Sytox green staining achieved IC50 values comparable to that of the larval motility assay. The cytotoxicity of the combined coriander oil and linalool on Madin–Darby Canine Kidney cells was evaluated using sulforhodamine-B (SRB) assay and showed no significant cytotoxic effect at concentrations < 1%. These results indicate that testing essential oils and their main components may help to find new potential anthelmintic compounds, while at the same time reducing the reliance on synthetic anthelmintics.

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“…[27] suggested that a dose of 5000 mg/kg of Artemisia campestris essential oil shows a high nematocidal activity (72.1% FECR) in H . contortus on day 7 post-treatment [28]. In this study, the decrease in EPG value may be caused by the presence of tannin and saponin in A. catechu .…”

Section: Discussionmentioning

confidence: 81%

In vitro and in vivo Areca catechu crude aqueous extract as an anthelmintic against Ascaridia galli infection in chickens

et al. 2019

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Aim: Intestinal parasites cause severe diseases at poultry farms, especially in developing countries, including Indonesia. Infections cause significant decreases in productivity at poultry farms. Ascaridia galli is a common nematode found in chickens with a prevalence of up to 92%. This study evaluates the in vitro and in vivo effects of Areca catechu crude aqueous extract (AAE) as an anthelmintic against A. galli. Materials and Methods: In the in vitro study, already-infected slaughtered chickens were collected from local slaughterhouses in Yogyakarta. The chicken intestines were carefully examined and transported to the Parasitological Laboratory of Gadjah Mada University, Yogyakarta. A. galli was collected from the intestines and put into a Petri dish containing 0.62% saline solution. Sixty A. galli individuals were soaked in different concentrations of the AAE for 3-6 h. The number of deceased A. galli was recorded by ascertaining whether they had ceased movement using a stereo microscope. The morphological characteristics of the parasites were studied. The in vivo study used 40 female chickens that were 1.5 months old and infected with the eggs of A. galli. Approximately 3000 infective eggs were found in each chicken. There were four treatment groups; Group A served as the control and was treated with Aquadestilata, Group B was treated with 26 mg/ml of AAE, Group C was treated with 79 mg/ml of AAE, and Group D was treated with 50 mg/ml of pyrantel pamoate. The AAE was applied using a pipette for 14 days. The observed parameters include the effect of the treatments on hematology, body weight (BW), and the number of A. galli eggs within each gram of fecal matter. Results: The AAE damaged the morphology, including the anterior end, posterior end, and vulva, of A. galli in vitro. The tegument of the anterior and posterior ends was slightly rougher, wrinkled, and damaged. The vulva and spicule ducts were irregular and indistinct. The average eggs per gram (EPG) in the untreated control increased in vivo from 1450±355.90 to 1975±325.96. The average EPG decreased from 1485±386.62 to 0±0.00 during 14 days of treatment of 79 mg/ml of AAE. The mean BW gain in the treated chickens was significantly (p<0.5) higher than the control. The treatment with 79 mg/ml of AAE increased the total erythrocyte count, total leukocyte count, hemoglobin, and packed cell volume values in the chickens. Conclusion: AAE can cause morphological changes and subsequent death in adult A. galli in vitro and effectively decrease ascariasis severity in chickens, therefore increasing overall BW in vivo.

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Chemical analyses and anthelmintic effects of Artemisia campestris essential oil

Cited by 28 publications

References 37 publications

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

In vitro and in vivo Areca catechu crude aqueous extract as an anthelmintic against Ascaridia galli infection in chickens

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Chemical analyses and anthelmintic effects of Artemisia campestris essential oil

Cited by 28 publications

References 37 publications

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Nematocidal Effects of a Coriander Essential Oil and Five Pure Principles on the Infective Larvae of Major Ovine Gastrointestinal Nematodes In Vitro

In vitro and in vivo Areca catechu crude aqueous extract as an anthelmintic against Ascaridia galli infection in chickens

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Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies

Chemical analyses and evaluation of the anthelmintic effects of Origanum majorana essential oil, in vitro and in vivo studies