Chelidonium majus is not hepatotoxic in Wistar rats, in a 4 weeks feeding experiment

Mazzanti, Gabriela; Sotto, Antonella Di; Franchitto, Antonio; Mammola, Caterina Loredana; Mariani, Paola; Mastrangelo, Sabina; Menniti‐Ippolito, Francesca; Vitalone, Annabella

doi:10.1016/j.jep.2009.09.004

Cited by 32 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Based on this data, C. majus has been banned from oral use in Germany and other European countries. There is also an animal study that found no hepatotoxicity at doses about 50-100 times higher than those generally used in humans [63]. An average daily oral dose of alkaloids (sanguinarine: chelerythrine 3:1) up to 5 mg / 1 kg animal body weight proved to be safe [88].…”

Section: Analgesic and Antispasmodic Activitymentioning

confidence: 99%

“…1,1-diphenyl-2-picrylhydrazyl radical scavenging assay or FRAP assay [61,62], this does not depend on the alkaloid content of the drug or transition metal element content [61]. There is also an animal study that reported a slight but significant reduction of glutathione level and SOD activity in the liver, after oral administration of a massive dose of C. majus (1.5-3 g / kg / day) [63]. These results suggest that, in spite of its intrinsic antioxidant properties, C. majus might compromise the hepatic antioxidant protection in case of overdose.…”

Section: Antioxidant Versus Pro-oxidant Activitymentioning

confidence: 99%

See 1 more Smart Citation

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Gîlcă

Găman

Panait

et al. 2010

Forsch Komplementmed

113

View full text Add to dashboard Cite

Chelidonium majus L. (family Papaveraceae), or greater celandine, is an important plant in western phytotherapy and in traditional Chinese medicine. Crude extracts of C. majus as well as purified compounds derived from it exhibit a broad spectrum of biological activities (antiinflammatory, antimicrobial, antitumoral, analgesic, hepatoprotective) that support some of the traditional uses of C. majus. However, herbal medicine also claims that this plant has several important properties which have not yet been scientifically studied: C. majus is supposed to have diuretic, antitussive and eye-regenerative effects. On the other hand, C. majus also has scientifically proven effects, e.g. anti-osteoporotic activity and radioprotection, which are not mentioned in traditional sources. Moreover, recent controversy about the hepatoprotective versus hepatotoxic effects of Chelidonium majus has renewed the interest of the medical community in this plant. This review is intended to integrate traditional ethno-medical knowledge and modern scientific findings about C. majus in order to promote understanding of its therapeutic actions as well as its toxic potential.

show abstract

Section: Analgesic and Antispasmodic Activitymentioning

confidence: 99%

Section: Antioxidant Versus Pro-oxidant Activitymentioning

confidence: 99%

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Gîlcă

Găman

Panait

et al. 2010

Forsch Komplementmed

113

View full text Add to dashboard Cite

show abstract

“…The procedures to obtain blood samples and the determination of liver function parameters were conducted as previously published by Mazzanti et al [13] . Serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenases, cholinesterase, total bilirubin, cholebilirubin, and main electrolytes (sodium, potassium, chlorine, calcium, and phosphorus) were determined by using a photometric and ISE method (COBAS 6000 system; Roche Diagnostics, Switzerland).…”

Section: Clinical Chemistrymentioning

confidence: 99%

“…20 with a light microscope (Leica DM 4500B), as previously described by Mazzanti et al [13,14] . Briefly, liver fragments were obtained from the right lobe and 4-m sections were cut and stained with Masson's trichromic staining and hematoxylin-eosin.…”

Section: Light Microscopy and Immunohistochemistrymentioning

confidence: 99%

See 1 more Smart Citation

<i>Cassia angustifolia</i> Extract Is Not Hepatotoxic in an in vitro and in vivo Study

Vitalone¹,

Giacomo²,

Sotto³

et al. 2011

Pharmacology

View full text Add to dashboard Cite

Background:Cassia angustifolia L. (senna) is traditionally used as a laxative. Its major components are sennosides that are responsible for the laxative effect. Senna is recommended for the short-term treatment of acute constipation. Nevertheless people use its preparations as self-medication, often for long periods, to treat chronic constipation thus exposing themselves to adverse reactions. Most reactions were associated with hepatotoxicity. Aims: The present study was aimed to evaluate the toxicity of a C. angustifolia leafextract (standardized at 60% of sennosides) on rat liver cells and the long-term effects on liver functions, in Wistar rats. Methods: Cytotoxicity was assessed in a buffalo normal rat liver cell line (BRL-3A) by the trypan blue assay and the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction test. In vivo effects were observed after oral administration of the extract for 4 or 8 weeks at doses of 12 and 58 mg/kg/day. At the end of treatment, animals were sacrificed, the postmortem examination was performed and serum was used for biochemical analysis. Liver samples were used for histomorphological and immunohistochemical examination along with the determination of oxidative stress parameters. Results and Conclusion: In BRL-3A cells, the extract was cytotoxic at concentrations that appear largely higher than those attainable in humans. In Wistar rats, the extract did not induce any significant change in all of the parameters tested. In summary, the present study indicates a lack of hepatotoxicity of senna at doses higher than those generally used in humans.

show abstract

A sensitive and selective liquid chromatography−tandem mass spectrometry method for simultaneous determination of five isoquinoline alkaloids from Chelidonium majus L. in rat plasma and its application to a pharmacokinetic study

Zhou¹,

Liu²,

Wang³

et al. 2013

J. Mass. Spectrom.

View full text Add to dashboard Cite

Chelidonium majus L. is one of the most important medicinal plants of the family Papaveraceae. Its pharmacological effects have been primarily attributed to the presence of a number of alkaloids. In the present study, a sensitive and selective liquid chromatography-tandem mass spectrometry method for simultaneous determination of five isoquinoline alkaloids from Chelidonium majus L. was developed and validated. The analytes (protopine, chelidonine, coptisine, sanguinarine and chelerythrine), together with the internal standard (palmatine), were extracted from acidified rat plasma with ethyl acetate-dichloromethane (4:1, v/v). Chromatographic separation was carried out on a Diamonsil C(18) column with an isocratic mobile phase consisting of acetonitrile and water (adjusted to pH 2.3 with formic acid) (30:70, v/v) at a flow rate of 0.4 ml/min. Mass spectrometric detection was performed by selected reaction monitoring mode via electrospray ionization source operating in positive ionization mode. The assay exhibited good linearity (r ≥ 0.9933) for all the analytes. The lower limits of quantification were 0.197-1.27 ng/ml using only 50 µl of plasma sample. The intra- and inter-day precisions were less than 11.9%, and the accuracy was between -6.3% and 9.3%. The method was successfully applied to the pharmacokinetic study of the five alkaloids in rats after intragastric administration of Chelidonium majus L. extract.

show abstract

Chelidonium majus is not hepatotoxic in Wistar rats, in a 4 weeks feeding experiment

Cited by 32 publications

References 31 publications

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

Chelidonium majus – an Integrative Review: Traditional Knowledge versus Modern Findings

<i>Cassia angustifolia</i> Extract Is Not Hepatotoxic in an in vitro and in vivo Study

A sensitive and selective liquid chromatography−tandem mass spectrometry method for simultaneous determination of five isoquinoline alkaloids from Chelidonium majus L. in rat plasma and its application to a pharmacokinetic study

Contact Info

Product

Resources

About