Chelidonium majus crude extract inhibits migration and induces cell cycle arrest and apoptosis in tumor cell lines

Deljanin, Milena; Nikolić, Marko G.; Baskić, Dejan; Todorović, D. M.; Djurdjević, Predrag; Zarić, Milan; Stanković, Milan; Todorović, Miloš; Avramovic, Dusko; Popović, Suzana

doi:10.1016/j.jep.2016.06.056

Cited by 35 publications

(20 citation statements)

References 27 publications

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“…Molecules 2020, 25, x; doi: FOR PEER REVIEW www.mdpi.com/journal/molecules thoroughly studied before in many in vitro and in vivo experiments [39][40][41][42], moreover, three Vinca alkaloids (vincristine, vinblastine, and vinorelbine) have been approved for use in cancer therapies in the United States [43]. The aim of this work was to obtain a green synthesized Ag-Mn nanocomplex with enhanced properties provided by the nanomaterials and the two plant extracts used individually and in combination, and to test that nanocomplex concerning its potential as topical treatments of melanoma without affecting the normal cells, such as keratinocytes.…”

Section: Molecules 2020 25 X For Peer Review 13 Of 24mentioning

confidence: 99%

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Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity

et al. 2020

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Medicinal plants are often used as reducing agents to prepare metal nanoparticles through green-synthesis due to natural compounds and their potential as chemotherapeutic drugs. Thus, three types of eco-friendly Ag-MnO2 nanoparticles (Ag-MnO2NPs) were synthesized using C. majus (CmNPs), V. minor (VmNPs), and a 1:1 mixture of the two extracts (MNPs). These NPs were characterized using S/TEM, EDX, XRD, and FTIR methods, and their biological activity was assessed in vitro on normal keratinocytes (HaCaT) and skin melanoma cells (A375). All synthesized NPs had manganese oxide in the middle, and silver oxide and plant extract on the exterior. The NPs had different forms (polygonal, oval, and spherical), uniformly distributed, with crystalline structures and different sizes (9.3 nm for MNPs; 10 nm for VmNPs, and 32.4 nm for CmNPs). The best results were obtained with VmNPs, which reduced the viability of A375 cells up 38.8% and had a moderate cytotoxic effect on HaCaT (46.4%) at concentrations above 500 µg/mL. At the same concentrations, CmNPs had a rather proliferative effect, whereas MNPs negatively affected both cell lines. For the first time, this paper proved the synergistic action of the combined C. majus and V. minor extracts to form small and uniformly distributed Ag-MnO2NPs with high potential for selective treatments.

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Section: Molecules 2020 25 X For Peer Review 13 Of 24mentioning

confidence: 99%

“…alkaloids for V. minor, such as vincamine [37,38]. The antitumor activity of these two plants has been thoroughly studied before in many in vitro and in vivo experiments [39][40][41][42], moreover, three Vinca alkaloids (vincristine, vinblastine, and vinorelbine) have been approved for use in cancer therapies in the United States [43].…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity

et al. 2020

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“…If the damage is too much to be fixed, apoptosis is induced [25] . In order to further assess the influence of DOX on cell proliferation, cell cycle distribution of the cells was explored.…”

Section: Talimentioning

confidence: 99%

Discrimination of the Effects of Doxorubicin on Two Different Breast Cancer Cell Lines on Account of Multidrug Resistance and Apoptosis

Öncül¹,

Ercan²

2017

pharmaceutical-sciences

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Oncul and Ercan: Difference of Breast Cancer Cells Regarding Apoptosis and MDRBreast cancer is one of the most common cancer types and malfunctioning proteins in apoptotic pathways are known to be the main contributors of cancer development. In this study MCF-7 and MDA-MB-231 breast cancer cell lines were treated with a potent chemotherapeutic agent, doxorubicin. IC 50 values were calculated to be 8306 and 6602 nM, respectively. Percentage of the cells in cell cycle arrest increased in a dose-dependent manner and was more evident in MDA-MB-231 cells. Apoptotic cell number increased in MCF-7 and Bax/Bcl-2 ratio was measured to be ~7 fold higher in cells treated with 200 nM of doxorubicin when compared to the control. Likewise, apoptotic cell rate increased in MDA-MB-231 cells and Bax/Bcl-2 ratio was increased by 2 fold compared to the control. Doxorubicin was also found to suppress Mdr-1 in a concentration-dependent manner and the outcome was more evident in MCF-7 cells. Drug efflux assay results were also shown to be consistent with Mdr-1 gene expression levels in both cell lines. Consequently it was concluded that doxorubicin directly influences apoptotic pathway and multidrug resistance in both MCF-7 and MDA-MB-231 cells even though data all together suggest that the effect is more significant on MDA-MB-231.

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“…As very recently reported, Deljanin and co-workers observed that C. majus extract showed a selective time-and dose-dependent increase in cytotoxicity in six human cancer cell lines (A549, H460, HCT 116, SW480, MDA-MB-231 and MCF-7) with IC 50 values ranging from 44.65 ± 3.66 to 213.35 ± 3.74 µg/mL. In the same study, the IC 50 value determined for human peripheral blood mononuclear cells (PBMCs) was above 500 μg/mL, which was the highest concentration tested [13]. A similar low sensitivity has been seen in PBMCs when treated with chelidonine and homochelidonine.…”

Section: The Effects Of Chelidonine On the Viability And Expression Omentioning

confidence: 67%

Chelidonine and Homochelidonine Induce Cell Death through Cell Cycle Checkpoints and MAP Kinase Pathways

Havelek

Seifrtová

Královec

et al. 2017

Natural Product Communications

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This study focuses on the comparative in vitro cytotoxicity of chelidonine and homochelidonine on human cancer and non-cancer cells. Both alkaloids produced a decrease in cellular growth in a dose-dependent manner exhibiting greater potency in cancer cells. The growth inhibitory effect was evidenced in both ovarian carcinoma A2780 and lung fibroblast MRC-5 cells by inducing G2 and mitotic phase cell cycle arrest. Results indicated that the extent of apoptosis induced by chelidonine and homochelidonine was correlated to sensitivity to the antiproliferative activity of the evaluated compounds. Western blotting suggested that the cellular toxicological mechanism of chelidonine is related to the differential upregulation of phospho-Chk2, p21 Cip1/Waf1 , phospho-ERK1/2 and phospho-p38 in various cell types, leading to alternations in the suppression of proliferation and either induction or prevention of apoptosis. Chelidonine showed the more potent effects and also affected the cell cycle checkpoints and MAPK signaling pathways within cells.

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Chelidonium majus crude extract inhibits migration and induces cell cycle arrest and apoptosis in tumor cell lines

Cited by 35 publications

References 27 publications

Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity

Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity

Discrimination of the Effects of Doxorubicin on Two Different Breast Cancer Cell Lines on Account of Multidrug Resistance and Apoptosis

Chelidonine and Homochelidonine Induce Cell Death through Cell Cycle Checkpoints and MAP Kinase Pathways

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