Checkerboard testing method indicates synergic effect of pelgipeptins against multidrug resistant Klebsiella pneumoniae

Costa, Rosiane Andrade da; Ortega, Daniel Barros; Fulgêncio, Débora Luíza Albano; Costa, Flávio Silva; Araújo, Thiago F.; Barreto, Cristine Chaves

doi:10.1016/j.biori.2018.12.001

Cited by 18 publications

(15 citation statements)

References 31 publications

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“…Even though the antibacterial activity of the four chemicals varied depending on the test pathogens, PGP-A and C displayed the strongest activity, followed by PGP-D and B. Our data differ from the results of previous reports ( Wu et al, 2010 ; Ding et al, 2011 ; Costa et al, 2019 ). This may be due to the different target bacteria, as the sensitivity of the bacteria to chemicals varies with the strains, even within one species.…”

Section: Discussioncontrasting

confidence: 99%

“…PGP-E isolated by P. elgii BC34-6 also showed strong antibacterial activity, including methicillin-resistant Staphylococcus aureus with MICs of 2–8 μg/ml ( Kim et al, 2018 ). In another report, PGP-D that was isolated from P. elgii AC13, showed the strongest antibacterial activity among the four types of PGP ( Costa et al, 2019 ). In this study, 4 PGPs, namely A, B, C, and D, were isolated from the fermentation supernatant of P. elgii JCK-5075, showed strong in vitro antibacterial activity against 14 plant pathogenic bacteria, with MIC values of 2–32 μg/ml.…”

Section: Discussionmentioning

confidence: 97%

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Biological Control of Tomato Bacterial Wilt, Kimchi Cabbage Soft Rot, and Red Pepper Bacterial Leaf Spot Using Paenibacillus elgii JCK-5075

Kim

et al. 2020

Front. Plant Sci.

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The over and repeated use of chemical bactericides to control plant bacterial diseases has resulted in unwanted effects, such as environmental pollution, residual toxicity, and resistance buildup in bacterial pathogens. Many previous studies have aimed to develop biological control agents to replace chemical bactericides. In this study, the antibacterial efficacy of the fermentation broth of Paenibacillus elgii JCK-5075 and its antibacterial compounds were evaluated against plant pathogenic bacteria, using both in vitro and in vivo bioassays. Pelgipeptins (PGPs) A, B, C, and D that were isolated from P. elgii JCK-5075 displayed broad-spectrum antibacterial activity against various plant pathogenic bacteria. The fermentation broth of P. elgii JCK-5075, at 5-fold dilution, effectively suppressed the development of tomato bacterial wilt, Kimchi cabbage soft rot, and red pepper bacterial leaf spot in pot experiments with control values of 81, 84, and 67%, respectively. PGP-A and C, at 200 μg/ml, were also found to markedly reduce the development of Kimchi cabbage bacterial soft rot by 75% and tomato bacterial wilt by 83%, respectively, and their disease control efficacy was comparable to that of oxolinic acid with control values of 81 and 85%, respectively. Additionally, the antibacterial activity of PGP-C was found to be directly correlated with membrane damage mechanisms. These results indicates that P. elgii JCK-5075 producing PGPs could be used as a biocontrol agent for the control of plant bacterial diseases. This is the first report on the in vitro and in vivo antibacterial activity of PGPs against bacterial plant pathogens.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Biological Control of Tomato Bacterial Wilt, Kimchi Cabbage Soft Rot, and Red Pepper Bacterial Leaf Spot Using Paenibacillus elgii JCK-5075

Kim

et al. 2020

Front. Plant Sci.

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“…Although both groups of lipopeptides present activity against bacteria [21] , [22] , our results show that they differ in their antifungal activity. Our bioassays show that pelgipeptins are effective against all pathogenic fungi tested, presenting MICs similar to fluconazole and consistently lower than MICs exhibited by polymyxin B.…”

Section: Discussionmentioning

confidence: 54%

“…The use of a mixture of pelgipeptin could be considered a substitute for the use of purified isoforms because there is a synergic effect between the pelgipeptin isoforms [22] . Moreover, obtaining pelgipeptin mix through a simple extraction in a solid phase, instead of repeated chromatographic runs, is a faster and less expensive alternative.…”

Section: Discussionmentioning

confidence: 99%

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<i>In vitro</i> antifungal activity of pelgipeptins against human pathogenic fungi and <i>Candida albicans</i> biofilms

Fulgêncio¹,

Costa²,

Guilhelmelli³

et al. 2021

AIMS Microbiology

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Systemic mycoses have become a major cause of morbidity and mortality, particularly among immunocompromised hosts and long-term hospitalized patients. Conventional antifungal agents are limited because of not only their costs and toxicity but also the rise of resistant strains. Lipopeptides from Paenibacillus species exhibit antimicrobial activity against a wide range of human and plant bacterial pathogens. However, the antifungal potential of these compounds against important human pathogens has not yet been fully evaluated, except for Candida albicans . Paenibacillus elgii produces a family of lipopeptides named pelgipeptins, which are synthesized by a non-ribosomal pathway, such as polymyxin. The present study aimed to evaluate the activity of pelgipeptins produced by P. elgii AC13 against Cryptococcus neoformans , Paracoccidioides brasiliensis , and Candida spp. Pelgipeptins were purified from P. elgii AC13 cultures and characterized by high-performance liquid chromatography (HPLC) and mass spectrometry (MALDI-TOF MS). The in vitro antifugal activity of pelgipeptins was evaluated against C. neoformans H99, P. brasiliensis PB18, C. albicans SC 5314, Candida glabrata ATCC 90030, and C. albicans biofilms. Furthermore, the minimal inhibitory concentration (MIC) was determined according to the CLSI microdilution method. Fluconazole and amphotericin B were also used as a positive control. Pelgipeptins A to D inhibited the formation and development of C. albicans biofilms and presented activity against all tested microorganisms. The minimum inhibitory concentration values ranged from 4 to 64 µg/mL, which are in the same range as fluconazole MICs. These results highlight the potential of pelgipeptins not only as antimicrobials against pathogenic fungi that cause systemic mycoses but also as coating agents to prevent biofilm formation on medical devices.

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A novel family of non-secreted tridecaptin lipopeptide produced by Paenibacillus elgii

et al. 2022

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Checkerboard testing method indicates synergic effect of pelgipeptins against multidrug resistant Klebsiella pneumoniae

Cited by 18 publications

References 31 publications

Biological Control of Tomato Bacterial Wilt, Kimchi Cabbage Soft Rot, and Red Pepper Bacterial Leaf Spot Using Paenibacillus elgii JCK-5075

Biological Control of Tomato Bacterial Wilt, Kimchi Cabbage Soft Rot, and Red Pepper Bacterial Leaf Spot Using Paenibacillus elgii JCK-5075

<i>In vitro</i> antifungal activity of pelgipeptins against human pathogenic fungi and <i>Candida albicans</i> biofilms

A novel family of non-secreted tridecaptin lipopeptide produced by Paenibacillus elgii

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