CHD4/NuRD maintains demethylation state of rDNA promoters through inhibiting the expression of the rDNA methyltransferase recruiter TIP5

Ling, Te; Xie, Wenbing; Luo, Min; Shen, Mengyan; Zhu, Qiaoyun; Zong, Le; Zhou, Tingting; Gu, Jun; Lu, Zhigang; Zhang, Feixiong; Tao, Wei

doi:10.1016/j.bbrc.2013.06.045

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…7c), indicating compromised rRNA biogenesis in Chd5 Aam1−/− spermatids. These results suggest a role of Chd5 in rRNA biogenesis during spermiogenesis, which is consistent with the known function of other CHD proteins such as CHD4 and CHD7 in positively regulating rRNA biogenesis 37,38 .…”

Section: Resultssupporting

confidence: 87%

Chd5 orchestrates chromatin remodelling during sperm development

Kim

et al. 2014

Nat Commun

108

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One of the most remarkable chromatin remodeling processes occurs during spermiogenesis, the post-meiotic phase of sperm development during which histones are replaced with sperm-specific protamines to repackage the genome into the highly compact chromatin structure of mature sperm. Here we identify Chromodomain helicase DNA binding protein 5 (Chd5) as a master regulator of the histone-to-protamine chromatin remodeling process. Chd5 deficiency leads to defective sperm chromatin compaction and male infertility in mice, mirroring the observation of low CHD5 expression in testes of infertile men. Chd5 orchestrates a cascade of molecular events required for histone removal and replacement, including histone 4 (H4) hyperacetylation, histone variant expression, nucleosome eviction, and DNA damage repair. Chd5 deficiency also perturbs expression of transition proteins (Tnp1/Tnp2) and protamines (Prm1/2). These findings define Chd5 as a multi-faceted mediator of histone-to-protamine replacement and depict the cascade of molecular events underlying chromatin remodeling during this process of extensive chromatin remodeling.

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Section: Resultssupporting

confidence: 87%

Chd5 orchestrates chromatin remodelling during sperm development

Kim

et al. 2014

Nat Commun

108

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“…The NuRD complex is activated in response to the attenuation of cell growth and establishes the poised state of rDNA, a chromatin state that is accessible for transcriptional factors but transcriptionally inactive, thereby suppressing rDNA transcription and maintaining the inactive rDNA (56). Binding of TTF-I to the T 0 element and to CSB (Cockayne syndrome protein B), a DNA-dependent ATPase, facilitates the recruitment of the NuRD complex to unmethylated CpG rDNA promoters (56,57). Interestingly, the NuRD complex also negatively regulates TIP5 expression, which leads to the suppression of CpG methylation at rDNA promoters (57).…”

Section: Pathways For Rdna Silencingmentioning

confidence: 99%

“…Binding of TTF-I to the T 0 element and to CSB (Cockayne syndrome protein B), a DNA-dependent ATPase, facilitates the recruitment of the NuRD complex to unmethylated CpG rDNA promoters (56,57). Interestingly, the NuRD complex also negatively regulates TIP5 expression, which leads to the suppression of CpG methylation at rDNA promoters (57). As such, the NuRD complex-mediated state of rDNA can be categorized into a poised state of rDNA chromatin or of rRNA genes, which functions in maintaining silent rDNA in growth-arrested or differentiated cells.…”

Section: Pathways For Rdna Silencingmentioning

confidence: 99%

“…As such, the NuRD complex-mediated state of rDNA can be categorized into a poised state of rDNA chromatin or of rRNA genes, which functions in maintaining silent rDNA in growth-arrested or differentiated cells. The NuRD complex maintains this poised state of rDNA chromatin by forming a repressive nucleosome on the rDNA core promoter, which is associated with bivalent histone modifications; a heterochromatic mark, trimethylated histone H3 at lysine 27 (H3K27me3); and a euchromatic mark, trimethylated histone H3 at lysine 4 (H3K4me3), and this complex also provides an intermediate configuration of rDNA chromatin, which is not transcribed but which remains transcription permissive (56,57).…”

Section: Pathways For Rdna Silencingmentioning

confidence: 99%

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The Epigenetic Pathways to Ribosomal DNA Silencing

Srivastava

Ahn

2016

Microbiol Mol Biol Rev

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SUMMARYHeterochromatin is the transcriptionally repressed portion of eukaryotic chromatin that maintains a condensed appearance throughout the cell cycle. At sites of ribosomal DNA (rDNA) heterochromatin, epigenetic states contribute to gene silencing and genome stability, which are required for proper chromosome segregation and a normal life span. Here, we focus on recent advances in the epigenetic regulation of rDNA silencing inSaccharomyces cerevisiaeand in mammals, including regulation by several histone modifications and several protein components associated with the inner nuclear membrane within the nucleolus. Finally, we discuss the perturbations of rDNA epigenetic pathways in regulating cellular aging and in causing various types of diseases.

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“…However, disruption of the NuRD complex impairs rRNA transcription. NuRD also suppresses the function of NoRC to maintain the rDNA promoter in an unmethylated state [17]. The poised rRNA promoter is occupied by the pre-initiation complex without RNA Polymerase I [15].…”

mentioning

confidence: 99%

SHPRH as a new player in ribosomal RNA transcription and its potential role in homeostasis of ribosomal DNA repeats

Lee¹,

Park

Kim

et al. 2017

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There are hundreds of copies of rDNA repeats in mammalian chromosomes and the ratio of active, poised, or inactive rDNA is regulated in epigenetic manners. Recent studies demonstrated that a post-DNA replication repair enzyme, SHPRH affects rRNA transcription by recognizing epigenetic markers on rDNA promoters and unveiled potential links between DNA repair and ribosome biogenesis. This study suggests that SHPRH could be a link between mTOR-mediated epigenetic regulations and rRNA transcription, while concomitantly affecting genomic integrity.

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CHD4/NuRD maintains demethylation state of rDNA promoters through inhibiting the expression of the rDNA methyltransferase recruiter TIP5

Cited by 12 publications

References 24 publications

Chd5 orchestrates chromatin remodelling during sperm development

Chd5 orchestrates chromatin remodelling during sperm development

The Epigenetic Pathways to Ribosomal DNA Silencing

SHPRH as a new player in ribosomal RNA transcription and its potential role in homeostasis of ribosomal DNA repeats

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