SHPRH as a new player in ribosomal RNA transcription and its potential role in homeostasis of ribosomal DNA repeats

Lee, Deokjae; Park, Jun-Hong; Kim, Shinseog; Lee, Seon-Gyeong; Myung, Kyungjae

doi:10.1080/21541264.2017.1381795

Cited by 5 publications

(5 citation statements)

References 32 publications

(47 reference statements)

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“…Hence, it was concluded that this represents a poised state, which is transcriptionally silent but remains permissive for transcriptional activation. Recently, the helicase SNF2 histone linker PHD RING helicase (SHPRH), a functional homolog of yeast Rad5, has been suggested to be responsible for sensing of poised rDNA promoters and for localizing Pol I to these promoters via interaction with H3K4me and H3K4me2 (Lee et al 2017 , 2018 ). Furthermore, the authors identified the mammalian target of rapamycin (mTOR) pathway implicated in this process, thus linking gene activation with environmental cues.…”

Section: Nucleolar Morphology and Regulation Of Gene Transcriptionmentioning

confidence: 99%

Nucleolus and chromatin

Schöfer

Weipoltshammer

2018

Histochem Cell Biol

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The nucleolus as site of ribosome biogenesis holds a pivotal role in cell metabolism. It is composed of ribosomal DNA (rDNA), which is present as tandem arrays located in nucleolus organizer regions (NORs). In interphase cells, rDNA can be found inside and adjacent to nucleoli and the location is indicative for transcriptional activity of ribosomal genes—inactive rDNA (outside) versus active one (inside). Moreover, the nucleolus itself acts as a spatial organizer of non-nucleolar chromatin. Microscopy-based approaches offer the possibility to explore the spatially distinct localization of the different DNA populations in relation to the nucleolar structure. Recent technical developments in microscopy and preparatory methods may further our understanding of the functional architecture of nucleoli. This review will attempt to summarize the current understanding of mammalian nucleolar chromatin organization as seen from a microscopist’s perspective.

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Section: Nucleolar Morphology and Regulation Of Gene Transcriptionmentioning

confidence: 99%

Nucleolus and chromatin

Schöfer

Weipoltshammer

2018

Histochem Cell Biol

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“…Its associated gene symbol is a tumor suppressor gene named SHPRH, which could be found in glioblastoma and could affect genomic integrity. 10,11 Our data illustrated that high expression hsa_circ_0005075 was observed in CRC samples and cells by quantitative real-time PCR (qRT-PCR), and high expression of hsa_circ_0005075 was correlated with clinical pathological characteristics and survival rate. Moreover, proliferation and invasion of CRC cells were impacted by the expression level of hsa_circ_0005075.…”

Section: Introductionmentioning

confidence: 81%

Hsa_circ_0005075 promotes the proliferation and invasion of colorectal cancer cells

Zhong

Gong

2019

Int J Biol Markers

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Background: Aberrant expression of circular RNAs (circRNAs) is implicated in tumorigenesis and disease progression. However, the underlying molecular mechanisms and physiological functions of hsa_circ_0005075 in human colorectal cancer are still poorly understood. Methods: Quantitative real-time PCR (qRT-PCR) was performed to detect the expression level of hsa_circ_0005075 in colorectal cancer. Correlations of hsa_circ_0005075 expression with pathological parameters and overall survival were assessed. CCK-8 and Transwell invasion assays were utilized to determine the effect of hsa_circ_0005075 on proliferation and invasion of colorectal cancer cells. Results: Hsa_circ_0005075 was overexpressed in colorectal cancer tissues compared with paracancerous tissues and intestinal polyps. Its expression level was associated with distal metastasis, invasion, tumor node metastasis stage, and tumor diameter in colorectal cancer, and was negatively correlated with overall survival of patients with colorectal cancer. Moreover, enforced expression of hsa_circ_0005075 potentiated the proliferation and invasive behavior of colorectal cancer cells. Conclusions: Our findings suggested that hsa_circ_0005075 expression was increased in colorectal cancer and might serve as a promising diagnostic mark and therapy target of colorectal cancer.

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“…SHPRH , a cancer suppressor gene, functions in DNA repair and regulates ribosomal RNA transcription 23–25. Circ-SHPRH is a circular RNA derived from SHPRH exons via back-splicing.…”

Section: Discussionmentioning

confidence: 99%

<p>Diagnostic and prognostic roles of circ-SHPRH for solid cancers: a meta-analysis</p>

Li¹,

Huang²,

Gong³

et al. 2019

OTT

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Background: Circ-SHPRH is a novel circular RNA which was down-regulated in various cancer tissues. Several recent studies hinted that circ-SHPRH might function as a diagnostic and prognostic biomarker for solid cancers. This meta-analysis was performed to determine the diagnostic and prognostic roles of circ-SHPRH for these solid cancers. Methods: Publications were searched in PubMed, MEDLINE, Web of Science, Embase, China National Knowledge Infrastructure (CNKI), and Wanfang database. Pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the summary receiver operating characteristic curve (SROC) (AUC) with 95% confidence interval (95% CI) were calculated to evaluate the diagnostic value of circ-SHPRH. Pooled hazard ratio (HR) with 95% CI was applied to assess the prognostic value of circ-SHPRH. Results: Nine eligible studies, including 4 for diagnosis and 5 for prognosis, were included in this meta-analysis. Pooled sensitivity, specificity, PLR, NLR, DOR, and AUC were 0.79 (95% CI: 0.73–0.84), 0.75 (95% CI: 0.69–0.81), 3.20 (95% CI: 2.53–4.06), 0.28 (95% CI: 0.22–0.36), 11.27 (95% CI: 7.57–16.79), and 0.84 (95% CI: 0.80–0.87), respectively. Pooled HR indicated that low circ-SHPRH expression was significantly associated with poor overall survival (OS) (low vs high, HR=2.22, 95% CI: 1.60–3.09). Conclusion: The results of this meta-analysis indicate that circ-SHPRH might be an important diagnostic and prognostic biomarker for various solid cancers.

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SHPRH as a new player in ribosomal RNA transcription and its potential role in homeostasis of ribosomal DNA repeats

Cited by 5 publications

References 32 publications

Nucleolus and chromatin

Nucleolus and chromatin

Hsa_circ_0005075 promotes the proliferation and invasion of colorectal cancer cells

<p>Diagnostic and prognostic roles of circ-SHPRH for solid cancers: a meta-analysis</p>

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