Chasing stress signals – Exposure to extracellular stimuli differentially affects the redox state of cell compartments in the wild type and signaling mutants of Botrytis cinerea

Marschall, Robert; Schumacher, Julia; Siegmund, Ulrike; Tudzynski, Paul

doi:10.1016/j.fgb.2016.03.002

Cited by 17 publications

(24 citation statements)

References 74 publications

(113 reference statements)

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“…They used a fungal Δbap1 mutant strain expressing radiometric redox-sensitive green fluorescent protein (roGFP2), to measure the dynamics of the glutathione pool in B. cinerea in vivo and in planta. This sensor was later used to study the temporal changes to redox state in various other mutants (Marschall et al, 2016a). Also, the glutaredoxin-bound GFP (Grx1-roGFP2) vector has been expressed in M. oryzae to understand the antioxidant defense system of the rice blast fungus in planta, with this work concluding that plant oxidative bursts were not sufficient to prevent infection (Samalova et al, 2014).…”

Section: Hyper Redox Sensorsmentioning

confidence: 99%

Reactive oxygen species metabolism and plant-fungal interactions

Segal

Wilson

2018

Fungal Genetics and Biology

155

103

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Fungal interactions with plants can involve specific morphogenetic developments to access host cells, the suppression of plant defenses, and the establishment of a feeding lifestyle that nourishes the colonizer oftenbut not always-at the expense of the host. Reactive oxygen species (ROS) metabolism is central to the infection process, and the stage-specific production and/or neutralization of ROS is critical to the success of the colonization process. ROS metabolism during infection is dynamicsometimes seemingly contradictory-and involves endogenous and exogenous sources. Yet, intriguingly, molecular decision-making involved in the spatio-temporal control of ROS metabolism is largely unknown. When also considering that ROS demands are similar between pathogenic and beneficial fungal-plant interactions despite the different outcomes, the intention of our review is to synthesize what is known about ROS metabolism and highlight knowledge gaps that could be hindering the discovery of novel means to mediate beneficial plant-microbe interactions at the expense of harmful plant-microbe interactions.

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Section: Hyper Redox Sensorsmentioning

confidence: 99%

Reactive oxygen species metabolism and plant-fungal interactions

Segal

Wilson

2018

Fungal Genetics and Biology

155

103

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“…Accordingly, the intracellular content of H 2 O 2 decreased when S. scitamineum cells were exposed to H 2 O 2 for 30 minutes, whereas after 180 minutes, H 2 O 2 content entered in a redox homeostasis restoring the initial environment. These results over again supported the earlier statement that an adaptive response is in course such as those seen for other systems (MARSCHALL et al, 2016). In C. albicans, for instance, the antioxidant system activation contributed to acquisition of an adaptive response to H 2 O 2 (JAMIESON et al, 1996;GONZÁLEZ-PÁRRAGA et al, 2003).…”

Section: Discussionsupporting

confidence: 87%

“…C. albicans has developed antioxidant systems, required for adaptation to ROS exposure from the host throughout evolution (Alonso-Monge et al, 2003;Herrero-de-Dios et al, 2010). This is also true for plant pathogens (MARSCHALL et al, 2016;MENTGES;BORMANN, 2015; MARSCHALL; TUDZYNSKI, 2014) and potentially for S. scitamineum, the cell redox state also changes when exposed to stress by means of an adaptative response and signaling to maintain its biotrophic lifestyle.…”

Section: Discussionmentioning

confidence: 98%

A more detailed view of reactive oxygen species metabolism in the sugarcane and <i>Sporisorium scitamineum</i> interaction

Peters¹

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Melhoramento de Plantas" for the opportunity to carry out my Doctor of Science studies. To God for the gift of life and for making me believe that we can make a better world. To my parents, Dircéa and Odilon, who are examples of life, love, character and faith. For understanding when I couldn't be there and for teaching me that dreams can come true. To my sister and brothers, Bethânia, Diogo and Danilo, and my niece Ana for the peace and joy they give me. To my love Moisés for the patience and companionship. To Prof. Claudia Barros Monteiro-Vitorello, for the commitment in making me a scientist. I am grateful for the advisement, mentoring, opportunities, patience and friendship. I feel honored and pleased to being a part of your team. Especially to Prof. Ricardo Antunes de Azevedo for the mentoring, trust and friendship that I will always have with me.

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“…Z-stacks were displayed as average projections via the CLSM software. Further evaluation was done with the Image J program (v.1.44f; http://rsb.info.nih.gov/ij/ ) as it was shown before [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

“…In fungi, Nox complexes exhibit distinct functions [ 1 , 49 ]. While the NoxA (Nox1) complex is controlling fruiting body formation [ 7 , 28 , 31 ], formation of sclerotia [ 14 , 40 , 42 ] and conidial anastomosis tubes (CATs) [ 38 ], virulence [ 23 , 42 , 53 ] and cellulose degradation [ 5 ], the NoxB (Nox2) complex is responsible for penetration of host tissue [ 12 , 42 ], the production of ROS [ 33 ] and ascospore germination [ 7 , 31 ]. However, the exact composition of the Nox complexes, their site of action as well as the integration in existing signaling pathways are still poorly elucidated.…”

Section: Introductionmentioning

confidence: 99%

Update on Nox function, site of action and regulation in Botrytis cinerea

Marschall

Siegmund

Burbank

et al. 2016

Fungal Biol Biotechnol

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BackgroundThe production of reactive oxygen species (ROS) and a balanced redox homeostasis are essential parameters, which control the infection process of the plant pathogen Botrytis cinerea. The necrotrophic fungus is able to cope with the plants’ oxidative burst and even produces its own ROS to overcome the plants’ defense barrier. Major enzyme complexes, which are responsible for the production of superoxide, are NADPH oxidase (Nox) complexes. They play a central role in various growth, differentiation and pathogenic processes. However, information about their regulation and the integration in the complex signaling network of filamentous fungi is still scarce.ResultsIn this work, we give an update on Nox structure, function, site of action and regulation. We show that functionality of the catalytic Nox-subunits seems to be independent from their transcriptional regulation and that the membrane orientation of BcNoxA would allow electron transport inside the ER. Following previous studies, which provided evidence for distinct functions of the NoxA complex inside the ER, we highlight in this work that the N-terminus of BcNoxA is essential for these functions. Finally, we elucidate the role of BcNoxD and BcNoxB inside the ER by complementing the deletion mutants with ER bound alleles.ConclusionsThis study provides a deeper analysis of the Nox complexes in B. cinerea. Besides new insights in the overall regulation of the complexes, we provide further evidence that the NoxA complex has a predominant role inside the ER, while the NoxB complex is mainly important outside the ER, likely at the plasma membrane. By considering all other putative Nox complex members, we propose a putative model, which describes the distinct complex pattern upon certain differentiation processes.Electronic supplementary materialThe online version of this article (doi:10.1186/s40694-016-0026-6) contains supplementary material, which is available to authorized users.

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Chasing stress signals – Exposure to extracellular stimuli differentially affects the redox state of cell compartments in the wild type and signaling mutants of Botrytis cinerea

Cited by 17 publications

References 74 publications

Reactive oxygen species metabolism and plant-fungal interactions

Reactive oxygen species metabolism and plant-fungal interactions

A more detailed view of reactive oxygen species metabolism in the sugarcane and <i>Sporisorium scitamineum</i> interaction

Update on Nox function, site of action and regulation in Botrytis cinerea

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