Charge Transport within a Three-Dimensional DNA Nanostructure Framework

Lü, Na; Pei, Hao; Ge, Zhilei; Simmons, C.R.; Yan, Hao; Fan, Chunhai

doi:10.1021/ja302447r

Cited by 117 publications

(107 citation statements)

References 37 publications

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“…DNA-based nanostructures 45,46 represent another means to enhance the sensitivity of electrochemical nucleic acid detection. Tetrahedral structures made of DNA oligonucleotides display probes with well-defined distances to promote accessibility and overall detection sensitivity (Fig.…”

Section: Nucleic Acid Detection With Record-breaking Speedmentioning

confidence: 99%

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

et al. 2014

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Rapid progress in identifying disease biomarkers has increased the importance of creating high-performance detection technologies. Over the last decade, the design of many detection platforms has focused on either the nano or micro length scale. Here, we review recent strategies that combine nano- and microscale materials and devices to produce large improvements in detection sensitivity, speed and accuracy, allowing previously undetectable biomarkers to be identified in clinical samples. Microsensors that incorporate nanoscale features can now rapidly detect disease-related nucleic acids expressed in patient samples. New microdevices that separate large clinical samples into nanocompartments allow precise quantitation of analytes, and microfluidic systems that utilize nanoscale binding events can detect rare cancer cells in the bloodstream more accurately than before. These advances will lead to faster and more reliable clinical diagnostic devices.

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Section: Nucleic Acid Detection With Record-breaking Speedmentioning

confidence: 99%

Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering

et al. 2014

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“…This change in the strength of fluorescence is typical of Au-quenched fluorescence, which is due to the energy transfer distance. Similarly, Lu et al 67 conjugated either methylene blue or ferrocene redox molecules to different positions of the surface-confined DNA tetrahedron and studied the throughduplex and through-space DNA-mediated charge transport. Such interesting properties are particularly useful for the design of molecular sensors for external stimuli.…”

Section: D-structured Dna Probesmentioning

confidence: 99%

Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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In addition to its fundamental function as a genetic code carrier, the utilization of DNA in various material applications has been actively explored over the past several decades. DNA is intrinsically an excellent type of self-assembly nanomaterial owing to its predictable base-pairing, high chemical stability and the convenience it possesses for synthesis and modification. Because of these unparalleled properties, DNA is widely used as excellent recognition elements in biosensors and as unique building blocks in nanodevices. A critical challenge in surface-based DNA biosensors lies in the reduced accessibility of target molecules to the DNA probes arranged on heterogeneous surfaces, especially when compared to probe-target recognition in homogeneous solutions. To improve the recognition abilities of these heterogeneous surface-confined DNA probes, much effort has been devoted to controlling the surface chemistry, conformation and packing density of the probe molecules, as well as the size and geometry of the surface. In this review, we aim to summarize the recent progress on the improvement of the probetarget recognition properties by introducing DNA nanostructure scaffolds. A range of new strategies have proven to provide a significantly enhanced range in the spatial positioning and the accessibility of the probes to the surface over previously reported linear structures. We will also describe the applications of DNA nanostructure scaffold-based biosensors. INTRODUCTIONDetection of nucleic acids (DNA or RNA) is an integral step in many applications, including in clinical diagnosis, environmental monitoring and antibioterrorism. 1 With these applications in mind, significant efforts have been made to develop sensitive, selective, rapid and cost-effective DNA (or RNA) biosensors. In parallel, DNAbased devices have also attracted a significant, recent interest owing to their promising applications in nanoelectronics, 2,3 biomolecular computations, 4-8 cellular imaging 9 and drug delivery. [10][11][12] In a typical design of DNA biosensors and nanodevices, oligonucleotides are often used as the molecular recognition layer. Because DNA is comprised of only four bases with A-T and G-C pairing, DNA hybridization processes are highly predictable and can be finely tuned with a rational design of the sequence. In addition, DNA oligonucleotides are usually easy to design, chemically stable and cost-effective.Understanding the physical structure of a DNA probe immobilized on a surface is critical in applications using DNA as a molecular recognition element. The properties of the DNA molecular recognition layer (such as selectivity, sensitivity and reproducibility) highly depend on the structure of the self-assembled DNA film. Modeling studies with thiolated DNA have demonstrated that efficient hybridization occurs in the well-controlled condition of surface-attached probes with large interprobe distances and upright conformations. [13][14][15][16][17][18][19][20][21][22][23][24]

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“…A number of techniques such as molecular biology (Dervan, 2001;Keren et al, 2002;Shen et al, 2014), electrochemistry (Lu et al, 2012;Xia et al, 2010), spectroscopy (Pan et al, 2013;Wang et al, 2014a) and colorimetry have been applied to achieve sensitive detection of nucleic acids. Up to now, various signal amplification strategies have been developed for the ultrasensitive sequence-specific detection of DNA.…”

Section: Introductionmentioning

confidence: 99%