Ultrasensitive electrochemical detection of avian influenza A (H7N9) virus DNA based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzyme nanowires

Yu, Yanyan; Chen, Zuanguang; Jian, Wensi; Sun, Duanping; Zhang, Beibei; Li, Xinchun; Yao, Meicun

doi:10.1016/j.bios.2014.09.080

Cited by 79 publications

(37 citation statements)

References 39 publications

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“…The detection limit was calculated to be 2.5 fM according to three times the standard deviation of the blank sample measurement. The proposed assay obtained comparable or much lower detection limit against previously reported methods (Table 2) [27][28][29][30][31][32]. Three factors attributed to the results: (1) the employment of the good electrical conductivity and biocompatibility of WS 2 -MWCNTs composite not only accelerate electron transfer but also improve cDNA loading amount on the electrode; (2) highly loaded enzyme molecules on the surface of electrode by HCR; (3) enzymatic catalytic reaction for H 2 O 2 and hydroquinol.…”

Section: Optimization Of Hcr Conditionssupporting

confidence: 50%

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Shuai

Liu

et al. 2016

Sensors and Actuators B: Chemical

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Section: Optimization Of Hcr Conditionssupporting

confidence: 50%

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Shuai

Liu

et al. 2016

Sensors and Actuators B: Chemical

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“…Das elektrochemische Signal wurde durch die alkalische Phosphatase produziert. [74] Sobald das Te mplat verdrängt wurde,initiierte der Tr igger-Oligo eine Hybridisierungskettenreaktion ("hybridization chain reaction", HCR) (Abbildung 2C). [72] Die Target-miRNAi nitiierte EXPAR, wodurch das Te mplat von der Elektrodenoberfläche verdrängt wurde und die Bildung des G-Quadruplex ermçglichte.D er G-Quadruplex besaß eine HRP-ähnliche Aktivität, wodurch ein elektrochemisches Signal produziert wurde.…”

Section: Elektrochemische Detektionunclassified

“…[73] Eine ähnliche Methode benutzte ein EXPAR-Templat, um einen verankerten Tr igger-Oligo zu blockieren. [74] Sobald das Te mplat verdrängt wurde,initiierte der Tr igger-Oligo eine Hybridisierungskettenreaktion ("hybridization chain reaction", HCR) (Abbildung 2C). [75,76] Die HCR generierte daraufhin viele G-Quadruplex-DNAzyme, die das elektrochemische Signal produzierten.…”

Section: Elektrochemische Detektionunclassified

Die exponentielle isotherme Amplifikation von Nukleinsäuren und Assays zur Detektion von Proteinen, Zellen, kleinen Molekülen und Enzymaktivitäten: Anwendungen für EXPAR

Reid¹,

Zhang

2018

Angewandte Chemie

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Isotherme exponentielle Amplifikationstechniken, wie die Strangverdrängungsamplifikation (SDA), die Rolling‐Circle‐Amplifikation (RCA), die Loop‐vermittelte isotherme Amplifikation (LAMP), die Nukleinsäuresequenz‐basierte Amplifikation (NASBA), die Helikase‐abhängige Amplifikation (HDA) und die Rekombinase‐Polymerase‐Amplifikation (RPA) besitzen großes Anwendungspotential für Vor‐Ort‐Analysen, patientennahe Labordiagnostik und In‐situ‐Assays. Diese Amplifikationsmethoden benötigen im Gegensatz zur Polymerasekettenreaktion (PCR) keine Temperaturzyklen, liefern aber vergleichbare Ausbeuten. In diesem Kurzaufsatz stellen wir die neuesten Entwicklungen der exponentiellen Amplifikationsreaktion (EXPAR) zur Detektion von Nukleinsäuren, Proteinen, Enzymaktivitäten, Zellen und Metallionen vor. Mithilfe der Verwendung von Fluoreszenz, Kolorimetrie, Chemilumineszenz, Raman und elektrochemischer Verfahren konnte bereits eine hoch sensitive Detektion einer Vielzahl verschiedener Ziele (Targets) realisiert werden. Dennoch bleiben offene Fragen, wie insbesondere die der unerwünschten Hintergrundamplifikation aus unspezifischen Templat‐Interaktionen, die es zu lösen gilt, um die Entwicklung der isothermen und exponentiellen Amplifikationstechniken weiter voranzutreiben.

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“…However, classical molecular methods incorporating PCR are laborious, technically demanding and do not provide a quick turnaround time from sample collection to results in outpatient clinics and emergency departments [10,16,17]. Non-PCR nucleic acid amplification-based techniques such as transcription-mediated amplification [18], nucleic acid sequence based amplification [19], single primer isothermal amplification [20], helicase-dependent amplification [21], and loop mediated isothermal amplification [22], have all been developed for detection of Flu. Use of a rapid and accurate diagnostic method for Flu such as these can enhance clinical management of influenza patients, prevent nosocomial outbreaks and help reduce the overuse of antibiotics [23,24].…”

Section: Introductionmentioning

confidence: 99%

Profile of the Alere i Influenza A & B assay: a pioneering molecular point-of-care test

Wang

Deng

Tang

2018

Expert Review of Molecular Diagnostics

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The Alere i Influenza A & B assay incorporates the Nicking Enzyme Amplification Reaction technique on the Alere i instrument to detect and differentiate influenza virus (Flu) A and B nucleic acids in specific specimens. Areas covered: The Alere i Influenza A & B assay was cleared by the US Food and Drug Administration for use with nasal swabs (NS) and nasopharyngeal swabs, either directly or in viral transport medium. Notably, direct use on NS was the first ever CLIA-waived nucleic acid-based test. Previously published evaluations have reported sensitivities and specificities of 55.2-100% and 62.5-100% for Flu A and 45.2-100% and 53.6-100% for Flu B, respectively. Expert commentary: The Alere i Influenza A & B assay provides a rapid and simple platform for detection and differentiation of Flu A and B. Efforts are expected to further improve sensitivity and user-friendliness for effective and widespread use in the true point-of-care setting.

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Ultrasensitive electrochemical detection of avian influenza A (H7N9) virus DNA based on isothermal exponential amplification coupled with hybridization chain reaction of DNAzyme nanowires

Cited by 79 publications

References 39 publications

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

An electrochemical biosensor for DNA detection based on tungsten disulfide/multi-walled carbon nanotube composites and hybridization chain reaction amplification

Die exponentielle isotherme Amplifikation von Nukleinsäuren und Assays zur Detektion von Proteinen, Zellen, kleinen Molekülen und Enzymaktivitäten: Anwendungen für EXPAR

Profile of the Alere i Influenza A & B assay: a pioneering molecular point-of-care test

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