Charge-independent effects of drugs on erythrocyte morphology

Nwafor, A Paul; Coakley, W. Terence

doi:10.1016/0006-2952(86)90082-1

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…Different techniques have demonstrated that therapeutic drugs can modify the structure and morphology of these cells (Nwafor and Coakley, 1986;Scheiman and Elta, 1990;Li et al, 1999;Shacter and Weitzman, 2002;Suwalsky et al, 2003;Hubner et al, 2005;Santos et al, 2005;Zhang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Maiworm¹,

Presta²,

Santos-Filho³

et al. 2008

Rev. bras. farmacogn.

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RESUMO: "Efeitos osmótico e morfológico na membrana de hemácias: ação de um extrato aquoso de Lantana camara". As infusões de folhas de Lantana camara (cambara-de-espinho) são usadas popularmente em alguns países para tratar doenças gastrointestinais. O experimento de fragilidade osmótica e a análise morfométrica têm sido usados para verifi car a interação de drogas com a membrana de hemácias. O objetivo deste trabalho foi avaliar os efeitos de um extrato aquoso de Lantana camara na fragilidade osmótica e na morfologia de hemácias. Amostras de sangue foram tratadas com extrato de Lantana camara (10 mg/mL), o ensaio de fragilidade osmótica e a análise morfológica foram realizadas. Na presença do extrato, os dados obtidos indicaram (i) um aumento signifi cativo (p < 0,05) da hemólise e (ii) modifi cações na morfologia das hemácias. Estes efeitos da Lantana camara poderiam estar associados com algumas propriedades farmacológicas de compostos químicos do extrato estudado.Unitermos: Lantana camara, Verbenaceae, morfologia, fragilidade osmótica, hemácias. ABSTRACT:The Lantana camara ("cambara de espinho") leaves infusions are used popularly in some countries to treat gastrointestinal diseases. Osmotic fragility assay and morphometric analysis have been used to verify the interaction of drugs with the membrane of red blood cells (RBC). The aim of this work was to evaluate the effects of an aqueous extract of Lantana camara on the osmotic fragility and on the morphology of RBC. Blood samples were treated with extract of Lantana camara (10 mg/mL), osmotic fragility assay and morphological analysis were carried out. In the presence of the extract, the data obtained indicated (i) a signifi cant (p < 0.05) increase of hemolysis and (ii) modifi cations on the morphology of RBC. These effects of the Lantana camara may be associated with some pharmacological properties of the chemical compounds of this studied extract.

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Section: Introductionmentioning

confidence: 99%

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Maiworm¹,

Presta²,

Santos-Filho³

et al. 2008

Rev. bras. farmacogn.

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show abstract

“…Red blood cells have been proposed as a prototypical cellular system regarding drug mediated plasma membrane effects (Li et al, 1999). Different techniques have demonstrated that therapeutic drugs can modify the structure and morphology of these cells (Nwafor and Coakley, 1986;Scheiman and Elta, 1990;Li et al, 1999;Shacter and Weitzman, 2002;Suwalsky et al, 2003;Hubner et al, 2005;Santos et al, 2005;Zhang et al, 2005). The morphometric analysis (area, shape and volume measurements) has been used to evaluate the alterations induced by natural products and synthetic drugs on membrane of red blood cells (Oliveira et al, 2002;Moreno et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Acetylsalicylic acid and morphology of red blood cells

Frydman

Fonseca

Rocha

et al. 2010

Braz. arch. biol. technol.

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“…Red blood cells have been proposed as a prototypical cellular system regarding drug mediated plasma membrane effects (Li et al, 1999). Different techniques have demonstrated that therapeutic drugs can modify the structure and morphology of these cells (Nwafor;Coakley, 1986;Scheiman;Elta, 1990;Li et al, 1999;Shacter;Weitzman, 2002;Suwalsky et al, 2003;Hubner et al, 2005;Santos et al, 2005;Zhang et al, 2005). The morphometric analysis (area, shape and volume measurements) has been used to evaluate the alterations induced by natural products and synthetic drugs on membrane of red blood cells (Oliveira et al, 2002;Moreno et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

Giani¹,

Paoli²,

Presta³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Determinação dos efeitos de uma fórmula usada na medicina tradicional chinesa (Buzhong Yi Qi Wan) na morfologia a fragilidade osmótica de hemácias". Buzhong Yi Qi Wan (BYQW) é uma combinação de algumas ervas medicinais amplamente usada na medicina tradicional chinesa para tratar o sangue, baço e desordens do estômago. A análise morfométrica e o ensaio de fragilidade osmótica têm sido usados para avaliar alterações na integridade da membrana de hemácias. O objetivo deste trabalho foi avaliar os efeitos de um extrato aquoso de BYQW na morfologia e na fragilidade osmótica de hemácias. Amostras sangüíneas foram tratadas com o extrato de BYQW, análise morfológica quantitativa/qualitativa e o ensaio de fragilidade osmótica foram realizados e comparados com grupo controle tratado com salina. Os dados obtidos indicaram ausência de modifi cações na morfologia, mas o ensaio de fragilidade osmótica sugeriu um aumento signifi cativo (p<0,05) da hemólise em hemácias isoladas de sangue tratado com extrato aquoso de BYQW. Em conclusão, o extrato aquoso de BYQW poderia afetar a integridade da membrana diminuindo a resistência osmótica sem alterar a forma das hemácias. Unitermos: Buzhong Yi Qi Wan, fragilidade osmótica, hemácias, morfologia.ABSTRACT: Buzhong Yi Qi Wan (BYQW) is a combination of some medicinal herbs widely used in traditional Chinese medicine to treat blood, spleen and stomach disorders. Morphometric analysis and osmotic fragility assay have been used to evaluate changes on membrane integrity of red blood cells. The aim of this work was to evaluate the effect of an aqueous BYQW extract on the morphology and osmotic fragility of red blood cells. Blood samples were treated with BYQW extract, quantitative/qualitative morphological analysis and osmotic fragility assay were carried out against control groups treated with saline. The data obtained indicated no modifi cation on morphology but osmotic fragility assay suggested a signifi cant (p<0.05) increasing of hemolysis in red blood cells isolated from blood treated with aqueous BYQW extract. In conclusion, the aqueous BYQW extract could affect the membrane integrity decreasing the osmotic resistance but without altering the shape of red blood cells.

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Charge-independent effects of drugs on erythrocyte morphology

Cited by 8 publications

References 12 publications

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Osmotic and morphological effects on red blood cell membrane: action of an aqueous extract of Lantana camara

Acetylsalicylic acid and morphology of red blood cells

Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

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