Charge-Controlled Synthetic Hyaluronan-Based Cell Matrices

Hegger, Patricia; Kupka, Julia; Minsky, Burcu Baykal; Boehm, Heike

doi:10.3390/molecules23040769

Cited by 5 publications

(7 citation statements)

References 24 publications

(38 reference statements)

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“…Moreover, hydrogels linked with the positively charged crosslinker displayed up to two times higher Young’s moduli. These interactions also affected the swelling ratios, which decreased with an increasing thiolation degree and with a reduced negative charge due to the use of the positively charged crosslinker [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…The instrument applies an increasing strain, between 0 and 10% and measures the resulting stresses. To obtain the respective Young’s moduli, these data were analyzed in the linear-viscoelastic region between 0 and 5% compression by a linear fit as described in [ 5 ]. For an exemplary graph of the linear fit of the stress–strain ratio of HA-DTPH 58% without and with the three different ionic cross-linker see Figure A2 of Appendix A .…”

Section: Methodsmentioning

confidence: 99%

“…A widely used example of an HA modification is the functionalization on the carboxyl groups of the glucuronic acid moiety with thiol groups (HA-DTPH) enabling disulfide bond formation and leading to hydrogels via oxidation with hydrogen peroxide [ 4 ]. Another cross-linking method of thiol modified HA is based on Michael thiol addition with different acrylate groups [ 5 ]. The mechanical properties of hydrogels based on thiol functionalized HA can be regulated through the molecular weight of HA, the percentage of modification and the ratio between thiol functionalized HA and used acrylate molecule.…”

Section: Introductionmentioning

confidence: 99%

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Small Physical Cross-Linker Facilitates Hyaluronan Hydrogels

2020

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In this study, we demonstrate that small charged molecules (NH4+, GluA+, dHA+) can form physical cross-links between hyaluronan chains, facilitating polymerization reactions between synthetically introduced thiol groups (HA-DTPH). These hybrid hydrogels can be obtained under physiological conditions ideally suited for 3D cell culture systems. The type and concentration of a physical crosslinker can be adjusted to precisely tune mechanical properties as well as degradability of the desired hydrogel system. We analyze the influence of hydrogen bond formation, concentration and additional ionic interactions on the polymerization reaction of HA-DTPH hydrogels and characterize the resulting hydrogels in regard to mechanical and biocompatibility aspects.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Small Physical Cross-Linker Facilitates Hyaluronan Hydrogels

2020

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“…weaker and reversible non-covalent interactions dominate over covalent bond formation. Many groups have reported HA- based materials that consist of chemically functionalizing the polymer backbone with moieties that can facilitate formation of supramolecular (such as in physiology) or covalent networks 9, 10 . Networks formed from unfunctionalized HA, however, have received considerably less attention in the literature.…”

Section: Introductionmentioning

confidence: 99%

“…Abstract: Self-assemblyHA has been crosslinked covalently via other mechanisms than photo-catalyzed 1 , 2 , 3 . Introduction: The authors mention that HA has been made into many covalently modified derivatives. Do they mean that functional groups were covalently bound to the HA backbone to make derivatives, or that functionalized HA can covalently bind to other molecules to form drug delivery and TE materials.…”

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Protein-mediated gelation and nano-scale assembly of unfunctionalized hyaluronic acid and chondroitin sulfate

et al. 2019

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Background: Hyaluronic acid (HA) is a major component of the extracellular matrix (ECM) in the central nervous system and the only purely supramolecular glycosaminoglycan. Much focus has been given to using this high molecular weight polysaccharide for tissue engineering applications. In most studies, the backbone of HA is functionalized with moieties that can facilitate network formation through physical self-assembly, or covalent crosslinking (e.g. photo-catalyzed) at concentrations where the polysaccharide does not gel on its own. However, these crosslinks often utilize functional groups not found in biological tissues. Methods: Oscillatory rheology, dynamic light scattering, and scanning electron microscopy were used to study albumin/HA structures. Dynamic light scattering and transmission electron microscopy were used to study albumin/chondroitin sulfate (CS) structures. UV-vis spectroscopy was used to demonstrate the potential for using protein-polymer blends as an ECM-mimetic model to study transport of small molecules. Results: We examine the intermolecular interactions of two major glycosaminoglycans found in the human brain, HA and the lower molecular weight CS, with the model protein albumin. We report the properties of the resulting micro- and nano materials. Our albumin/HA systems formed gels, and albumin/CS systems formed micro- and nanoparticles. These systems are formed from unfunctionalized polysaccharides, which is an attractive and simple method of forming HA hydrogels and CS nanoparticles. We also summarize the concentrations of HA and CS found in various mammalian brains, which could potentially be useful for biomimetic scaffold development. Conclusions: Simple preparation of commercially available charged biomacromolecules results in interesting materials with structures at the micron and nanometer length-scales. Such materials may have utility in serving as cost-effective models of nervous system electrostatic interactions and as in vitro drug release and model system for ECM transport studies.

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