Small Physical Cross-Linker Facilitates Hyaluronan Hydrogels

Erikci, Saliha; Mundinger, Patricia; Boehm, Heike

doi:10.3390/molecules25184166

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…Moreover, the absence of nuclear structures in HE staining was an indicator of effective cell removal during the decellularization process, and the extracellular matrix components type I collagen, type IV collagen, and laminin do not appear to be disrupted following decellularization according to qualitative and quantitative data ( Figure 2 ). These contents were ideally suited as 3D cell culture model systems mimicking the ECM and could be further supplemented with ECM proteins or adhesive peptides [ 38 ]. As shown in Figure 3 , results revealed by electron microscopy that the structure of the DPP was intact, and the epithelial cells were not identified.…”

Section: Discussionmentioning

confidence: 99%

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Chen

Deng

Luo

et al. 2022

Stem Cells International

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Seeking for suitable conjunctival reconstruction substitutes to overcome the limitations of current substitutes, such as amniotic membrane, is urgent. Decellularized tissues have become a promising strategy for tissue engineering. In this study, we prepared decellularized porcine pericardium (DPP) scaffolds by the phospholipase A2 method and crosslinked them with aspartic acid (Asp) and human endothelial growth factor (hEGF) to enhance biological performance on the DPP, obtaining DPP-Asp-hEGF scaffolds. In vitro DPP showed lower apoptosis, highly desirable, well preservation of extracellular matrix components, and favorable macro-microstructure, which was confirmed by histology, immunofluorescence, electron microscopy, collagen and DNA quantification, and cytotoxicity assay, compared to the native porcine pericardium (NPP). The crosslinked efficacy of the DPP-Asp-hEGF was furtherer verified by in vitro experiments with Fourier transform infrared (FTIR) and X-ray diffraction (XRD). Through animal models of conjunctiva defect model, the DPP-Asp-hEGF revealed a closed, multilayer epithelium with an equal amount of goblet cells and no indication for conjunctival scarring after 28 days, compared to amniotic membrane (AM) groups and sham groups. These results suggested that DPP-Asp-hEGF can offer a good conjunctival reconstructive substitute both in structure and in function.

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Section: Discussionmentioning

confidence: 99%

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Chen

Deng

Luo

et al. 2022

Stem Cells International

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“…Hence, the method of physical cross-linking has received more and more attention from researchers. Physically cross-linked hydrogels have the advantages of non-toxicity, good mechanical properties, strong cell adhesion, and slow degradation, which make them suitable for bone defect bioengineering scaffolds for bone regeneration [44][45][46][47]. Physical hydrogel refers to the hydrogel formed by the interaction of non-covalent forces between polymer chains, such as hydrogen bonding, ionic force, van der Waals interaction, polyelectrolyte complexation, stereocomplexation, and hydrophobic force [48].…”

Section: Physically Cross-linked Hydrogelsmentioning

confidence: 99%

Hydrogel Tissue Bioengineered Scaffolds in Bone Repair: A Review

Ding,

Zhang,

Liu

et al. 2023

Molecules

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Large bone defects due to trauma, infections, and tumors are difficult to heal spontaneously by the body’s repair mechanisms and have become a major hindrance to people’s daily lives and economic development. However, autologous and allogeneic bone grafts, with their lack of donors, more invasive surgery, immune rejection, and potential viral transmission, hinder the development of bone repair. Hydrogel tissue bioengineered scaffolds have gained widespread attention in the field of bone repair due to their good biocompatibility and three-dimensional network structure that facilitates cell adhesion and proliferation. In addition, loading natural products with nanoparticles and incorporating them into hydrogel tissue bioengineered scaffolds is one of the most effective strategies to promote bone repair due to the good bioactivity and limitations of natural products. Therefore, this paper presents a brief review of the application of hydrogels with different gel-forming properties, hydrogels with different matrices, and nanoparticle-loaded natural products loaded and incorporated into hydrogels for bone defect repair in recent years.

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“…In PBS, the hydrogels reached swelling equilibrium within 6 h and the swelling rate was 6.0 ± 0.35%. Astonishingly, the swelling rate of hydrogels in urine decreased to 3.2 ± 0.34%, which may be due to the high concentration of ions in urine, such as a large number of sodium ions and calcium ions [40]. Hydrogels with low swelling ratio possess high structural stability [41].…”

Section: Stability and Intravesical Retention Of Ha-sh/dmso Hydrogelsmentioning

confidence: 99%

Sulfhydryl functionalized hyaluronic acid hydrogels attenuate cyclophosphamide-induced bladder injury

Qiu¹,

Li²,

Huang³

et al. 2022

Biomed. Mater.

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Clinical management of cyclophosphamide (CYP) results in numerous side effects including hemorrhagic cystitis (HC), which is characterized by inflammation and oxidative stress damage. Intravesical hyaluronic acid (HA) supplementation, a therapeutic method to restore barrier function of bladder, avoid the stimulation of metabolic toxicants on bladder and reduce inflammatory response, has shown good results in acute or chronic bladder diseases. However, there are unmet medical needs for the treatment of HC to temporarily restore bladder barrier and reduce inflammation. Herein, sulfhydryl functionalized HA (HA-SH) and dimethyl sulfoxide (DMSO) were used to prepared a hydrogel system for optimizing the treatment of HC. We systematically evaluated the physicochemical of hydrogels and their roles in a rat model of CYP-induced HC. The prepared hydrogels exhibited outstanding gel forming properties, injectability, and biosafety. Swelling and retention studies showed that hydrogels were stable and could prolong the residence time of HA in the bladder. Histopathology and vascular permeability studies indicated that the hydrogels significantly attenuated bladder injury caused by CYP administration. Moreover, the hydrogels also showed excellent anti-inflammation and anti-oxidation properties. In conclusion, these data suggest that intravesical instillation of HA-SH/DMSO hydrogels reduces CYP-induced bladder toxicity and this work provides a new strategy for the prevention and early treatment of HC.

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Small Physical Cross-Linker Facilitates Hyaluronan Hydrogels

Cited by 5 publications

References 14 publications

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Crosslinked Decellularized Porcine Pericardium as a Substrate for Conjunctival Reconstruction

Hydrogel Tissue Bioengineered Scaffolds in Bone Repair: A Review

Sulfhydryl functionalized hyaluronic acid hydrogels attenuate cyclophosphamide-induced bladder injury

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