Characterizing the differentiation of osteoprogenitor cells on surface modified polyether-ether-ketone

Chen, Ya Shum; Lin, Jinru; Wu, Yu Ren; Chang, Chin Wei; Chang, Kai‐Chi; Chen, Chun Cheng; Chen, Chih Hua; Chen, Wen Cheng

doi:10.1016/j.surfcoat.2018.03.071

Cited by 10 publications

(3 citation statements)

References 29 publications

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“…SPEEK and GO-SPEEK show new absorption peaks at 1,050 cm −1 and 1,251 cm −1 . The 1,050 cm −1 peak corresponds to the S=O symmetric stretching absorption peak, and the 1,251 cm −1 peak corresponds to the O=S=O asymmetric stretching absorption peak ( Zhao et al, 2013 ; Chen et al, 2018 ). This indicates that the -SO 3 H functional group has been introduced into the PEEK polymer chain by the sulfonation reaction ( Zhao et al, 2013 ).…”

Section: Resultsmentioning

confidence: 99%

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

Huang,

Gu,

Yuan

et al. 2024

Front. Bioeng. Biotechnol.

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Poly-ether-ether-ketone (PEEK) is a biomedical plastic that can be used for orthopedic implants, but it offers poor antibacterial properties and bioactivity. In this study, PEEK was sulfonated with the obtained porous structure adsorbing graphene oxide (GO). The surface microstructures and properties of the original PEEK, sulfonated PEEK (SPEEK), and GO-grafted PEEK (GO-SPEEK) were characterized. The results revealed that the GO-SPEEK surface is a 3D porous structure exhibiting superior hydrophilicity to the original PEEK. Although SPEEK was shown to possess antimicrobial properties against both Escherichia coli and Staphylococcus aureus, the bactericidal effect was even more significant for GO-SPEEK, at about 86% and 94%, respectively. In addition, the in vitro simulated-body-fluid immersion and cell experiments indicated that GO-SPEEK had much better hydroxyapatite (HA)-precipitation induction capacity and cell–material interactions (e.g., cell adhesion, proliferation, osteodifferentiation, and extracellular matrix mineralization. The tensile test revealed that the mechanical properties of PEEK were maintained after surface modification, as GO-SPEEK has comparable values of elastic modulus and tensile strength to PEEK. Our investigation sought a method to simultaneously endow PEEK with both good antimicrobial properties and bioactivity as well as mechanical properties, providing a theoretical basis for developing high-performance orthopedic implants in the clinic.

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Section: Resultsmentioning

confidence: 99%

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

Huang,

Gu,

Yuan

et al. 2024

Front. Bioeng. Biotechnol.

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“…37 It has been reported that hydrophilic surface can stimulate cell growth and differentiation. 38,39 In contrast, Zhang et al. found that hydrophobic Polydimethylsiloxane (PDMS)-grafted Poly(vinyl chloride) (PVC) showed more effective on cell attachment and proliferation than that of hydrophilic Poly(ethylene glycol) (PEG)-grafted PVC.…”

Section: Resultsmentioning

confidence: 99%

Bioactive poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration

Zhou

et al. 2019

J Biomater Appl

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Microparticles with porous structure can be applied as microcarriers for both cell culture and tissue regeneration. While well-controlled pore structure represents a critical challenge to be achieved. In the present study, in order to develop microcarriers for cell culture, a series of poly(ε-caprolactone) microspheres were fabricated with varied macroporous structures. Poly(ε-caprolactone) microspheres were prepared via the integration of the emulsion/solvent evaporation and particle leaching mechanisms. Particularly, by adjusting poly(ε-caprolactone) concentration and the ratio between the porogen paraffin and poly(ε-caprolactone), the microspheres with the pore size of 25.6–84.0 μm and the porosity of 57.4–75.5% were obtained. Further, the microspheres were subjected to alkaline hydrolysis, followed by surface coating with hydroxyapatite. These porous poly(ε-caprolactone) microspheres with surface modification well supported the adhesion and growth of human fibroblasts. Together, bioactive poly(ε-caprolactone) microspheres with controlled pore structure are potential to be applied in cell culture and tissue regeneration.

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“…Medical-grade PEEK, which was purchased from Grand Ware Trading Co., Ltd. (Taipei, Taiwan), was processed based on our published research [ 26 ]. In standardizing the surface conditions of the PEEK sample, the samples were prepared at a flat disk with dimensions of 8 mm in diameter and 1 mm in thickness through a precision cutting machine (CL40, Top Tech Machines Co., Ltd., Taichung, Taiwan).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the Grafting Efficacy of Active Biomolecules of Phosphatidylcholine and Type I Collagen on Polyether Ether Ketone: In Vitro and In Vivo

Chen

Chang

et al. 2021

Polymers

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Biomolecule grafting on polyether ether ketone (PEEK) was used to improve cell affinity caused by surface inertness. This study demonstrated the sequence-polished (P) and sulfonated (SA) PEEK modification to make a 3D structure, active biomolecule graftings through PEEK silylation (SA/SI) and then processed with phosphatidylcholine (with silylation of SA/SI/PC; without SA/PC) and type I collagen (COL I, with silylation of SA/SI/C; without SA/C). Different modified PEEKs were implanted for 4, 8, and 12 weeks for histology. Sulfonated PEEK of SA showed the surface roughness was significantly increased; after the silylation of SA/SI, the hydrophilic nature was remarkably improved. The biomolecules were effectively grafted through silylation, and the cells showed improved attachment after 1 h. Furthermore, the SA/SI/PC group showed good in vitro mineralization. The new bone tissues were integrated into the 3D porous structures of SA/SI/PC and SA/SI/C in vivo making PEEK a potential alternative to metals in orthopedic implants.

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Characterizing the differentiation of osteoprogenitor cells on surface modified polyether-ether-ketone

Cited by 10 publications

References 29 publications

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

A self-assembling graphene oxide coating for enhanced bactericidal and osteogenic properties of poly-ether-ether-ketone

Bioactive poly(ε-caprolactone) microspheres with tunable open pores as microcarriers for tissue regeneration

Evaluation of the Grafting Efficacy of Active Biomolecules of Phosphatidylcholine and Type I Collagen on Polyether Ether Ketone: In Vitro and In Vivo

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