Characterizing heat shock protein 90 gene of Apolygus lucorum (Meyer-Dür) and its expression in response to different temperature and pesticide stresses

Abstract

In this study, we cloned a full-length cDNA of heat shock protein (HSP) gene of Apolygus lucorum (Meyer-Dür) [AlHSP90, KC109781] and investigated its expression in response to temperature and pesticide stresses. The open reading frame (ORF) of AlHSP90 is 2,169 bp in length, encoding a 722 amino acid polypeptide with a predicted molecular weight of 82.99 kDa. Transcriptional and translational expression profiles of AlHSP90 under extreme temperature or pesticide stresses were examined by fluorescent realtime quantitative PCR and Western blot. Results showed that the expression profiles of AlHSP90 protein were in high agreement with those of AlHSP90 RNA and indicated that AlHSP90 was not only an important gene for A. lucorum adults in response to extremely high temperature, but also involved in the resistance or tolerance to cyhalothrin, imidacloprid, chlorpyrifos, and emamectin benzoate, especially for female adults to emamectin benzoate and for male adults to cyhalothrin. Transcriptional results of AlHSP90 also confirmed that AlHSP90 was an important gene involved in the resistance or tolerance to both temperature and pesticide stresses. In addition, our study also revealed that ∼24°C may be the suitable temperature range for A. lucorum survival, which is also confirmed by the results of the expression of AlHSP90, the nymph mortality, and the intrinsic rate of increase (r m ) when A. lucorum is reared at six different temperatures. Therefore, these studies are significant in elucidating the AlHSP90 in response to temperature and pesticide stresses and would provide guidance for A. lucorum management with different pesticides or temperatures in fields.

Induced expression of three heat shock proteins mediated by thermal stress in Heortia vitessoides (Lepidoptera: Crambidae)

“…Furthermore, the presence of the MEEVD motif at the C‐terminus, which is the core sequence required for binding to the tetratricopeptide repeat domain of HSP90 co‐chaperones (Pearl & Prodromou ; Sun et al . ), is a characteristic shared by all eukaryotic HSP90 proteins.…”

“…Induction of HSP60, HSP70, and HSP90 may provide protection against this type of environmental stress (Sun et al . ; Xu et al . ; Sun et al .…”

“…In addition, the HSP90 mRNA and protein are highly expressed in response to heat shock, heavy metal pollution, a diapause, proteotoxic stresses, starvation stresses, and pesticide stresses (Sun et al . ).…”

Expression of heat-shock protein genes in Apis mellifera meda (Hymenoptera: Apidae) after exposure to monoterpenoids and infestation by Varroa destructor mites (Acari: Varroidae)

“…In an attempt to characterize the detoxifi cation mechanisms in stressed honey bees, Du Rand et al (2015) report that active detoxifi cation of nicotine by A. mellifera scutellata is associated with metabolic and proteomic responses such as up-regulation of heat shock proteins. Sun et al (2014) studied the level of expression of hsp90 in Apolygus lucorum (Heteroptera: Miridae) in response to different stressors such as pesticides, including cyhalothrin, imidacloprid, chlorpyrifos and emamectin benzoate.…”

Identification and characterization of heat shock proteins in a parasitic wasp Chouioia cuneae (Hymenoptera: Eulophidae)

“…Although HSPs have been studied in a number of insects, these studies have focused mainly on pests, such as Plutella xylostella (Chen & Zhang ), Apolygus lucorum (Sun et al ), Mamestra brassicae (Sonoda & Tsumuki ), Tribolium castaneum (Xu et al ), Spodoptera litura (Shu et al ) , Sarcophaga crassipalpis (Michaud et al ), Musca domestica (Tang et al ) etc. Therefore, not much is known about HSPs in the natural enemies of insects, especially parasitoids.…”