Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry

Ali, Yeasmeen; Islam, Md. Ashraful; Muzahid, Nazmul Hasan; Sikder, Mohd Omar Faruk; Hossain, M.A.; Marzan, Lolo Wal

doi:10.1016/j.apjtb.2016.12.001

Cited by 30 publications

(36 citation statements)

References 28 publications

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“…aureus isolated from our chicken meat samples were resistant to oxytetracycline (71.79%), azithromycin (64.10%) and erythromycin (58.97%); and sensitive to amikacin (100%), neomycin (100%), amoxicillin (94.87%), gentamicin (82.05%) and ciprofloxacin (71.79%). Similar findings also reported by other authors (Islam et al 2016;Islam et al 2014;Ali et al 2017). S. aureus isolated from our milk sample were sensitive to neomycin (100%), gentamicin (92%) ciprofloxacin (92%), amoxicillin (88%), doxycycline (88%) azithromycin (84%) and oxytetracycline(80%), and resistant to amikacin (56%) and erythromycin (44%).…”

supporting

confidence: 91%

“…Madubuike 2007). A number of infectious diseases such as dermatitis, pneumonia, meningitis, osteomyelitis in human, bovine mastitis in cattle and bumble foot disease in poultry (Ali et al, 2017) are produced by enterotoxigenic strains of coagulase-positive staphylococci, mainly S. aureus (Hennekinne et al, 2012). Among the food borne outbreaks bacterial toxin were 9.8% of which 55.4% cases were due to staphylococcal enterotoxins (Anonymous, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Multi–drug resistant Staphylococcus aureus isolated from milk, chicken meat, beef and egg in Bangladesh

Rahman

Islam

et al. 2018

Res. Agric. Livest. Fish.

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Staphylococcal infection is one of the most common food-borne diseases in the world. Moreover, antimicrobial resistance of pathogenic bacteria, including Staphylococcus (S.) aureus is an emerging problem of food safety. This study was conducted to investigate the prevalence of S. aureus in milk, chicken meat, egg and beef; and to determine the multi-drug resistance (MDR) profile of S. aureus in Mymensingh and Gazipur districts, Bangladesh. A total of 189 samples of milk (n=108), chicken meat (n=51), egg (n=20) and beef (n=10) were collected from Bangladesh Agricultural University dairy farm, American dairy farm, Gazipur and different dairy farms of municipal area and retail shops during July 2016 to June 2018. S. aureus were isolated and identified by conventional methods and polymerase chain reaction (PCR). Antimicrobial susceptibility tests were done through disc diffusion test using 10 commonly used antibiotics. The overall prevalence of S. aureus in all food samples was 43.39%. A total of 39 (76.47%) chicken meat, 25 (23.15%) milk, 11(55%) egg and 07 (70%) beef samples were S. aureus positive through conventional method. Among 82 culture positive samples only 39 samples (47.56%) were confirmed by PCR. Antibiogram study showed that S. aureus isolated from chicken meat were mostly resistant to oxytetracycline (71.79%); and highly sensitive to amikacin (100%) and neomycin (100%). S. aureus isolated from milk samples were highly sensitive to neomycin (100%), and resistant to amikacin (56%). Only 28.57% isolates of S. aureus originated from beef samples were resistant to oxytetracycline and 100 % isolates were sensitive to ciprofloxacin, gentamicin, erythromycin, azithromycin, doxycycline. Similarly, S. aureus isolated from egg samples were resistant to erythromycin (81.82%) and 100% sensitive to amikacin. Out of 41.46% MDR isolates 12%, 53.85%, 90.91% and 0% of the S. aureus originated from milk, chicken meat, egg and beef respectively. The higher prevalence of S. aureus in chicken meat, beef, egg and milk indicates unhygienic production, marketing and processing of these foods. Presence of MDR S. aureus in these foods might pose serious public health threats. Rational use of antibiotics with higher sensitivity should be prescribed in managing poultry diseases to reduce re-emerging MDR in Bangladesh.Res. Agric., Livest. Fish.5(2): 175-183, August 2018

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confidence: 91%

Section: Introductionmentioning

confidence: 99%

Multi–drug resistant Staphylococcus aureus isolated from milk, chicken meat, beef and egg in Bangladesh

Rahman

Islam

et al. 2018

Res. Agric. Livest. Fish.

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“…Several pathogenic diseases such as dermatitis, pneumonia, septicemia, osteomyelitis, and meningitis can cause infections both in humans and swine. Also, bovine mastitis in cattle and bumblefoot disease in poultry can be caused by enterotoxigenic strains of coagulase-positive staphylococci, mainly S. aureus [8,9]. Staphylococcal disease association varies with the source of infection (farms and other places) or infection in different body parts such as skin, muscles and yolk sac.…”

Section: Introductionmentioning

confidence: 99%

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Akhi

Das

Banik

et al. 2019

MRJI

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Staphylococcus aureus is gram-positive cocci that can cause foodborne illness which can be transmitted by chicken meat, both raw and undercooked; consumption of which may cause infection and/or toxicity in consumers. This current study was conducted for the detection of the prevalence of S. aureus in three types of poultry samples which included chicken meat, chicken eggs and droppings. Samples were aseptically collected from different rural and urban areas of 8 districts of Bangladesh in triplicate collection method which was conducted in the Centre for Excellence, Department of Microbiology, Primeasia University, Dhaka, Bangladesh to detect the Multi Drug-Resistant (MDR) S. aureus. Isolation was done by using 7.5% Sodium Chloride broth for enrichment and Mannitol salt agar after enriching samples in Buffer Peptone water. Antibiogram was done by using Kirby Bauer Method on Muller Hinton agar. The current study revealed 68% (17/25), 84% (21/25) and 52% (13/25) of S. aureus in chicken meat, eggs and dropping, respectively. Around 75.47% isolates (40/53) showed 100% similarity with S. aureus from the generated Dendrogram based on biochemical data. Cefixime (62.67%) showed the highest sensitivity against all of the isolates while Penicillin (86.62%) exhibited the highest resistance. Moreover, 59.62% of isolates were resistant to 5 or more drugs (Multidrug-Resistant). Poultry products such as meat and eggs were contaminated with drug-resistant S. aureus which can cause serious health effect to the consumer. The drug-resistant pathogen in droppings helps to indicate the spread of drug-resistant isolates in the environment. Therefore, proper sanitation measures should be taken to ensure the quality of the products during slaughtering, collecting eggs or disposing of poultry litter.

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“…Widespread and unauthorized administration of antimicrobials and disinfectant solutions in both medicine and veterinary have been considered to be a major factor in the emergence of antibiotic resistance amongst S. aureus bacteria. Similarly, considerable incidence of resistance of S. aureus bacteria recovered from chicken meat samples toward penicillin, tetracycline, cephalothin, cipro oxacin, erythromycin, mupirocin, clindamycin and trimethoprim/sulfamethoxazole antibiotic agents was conveyed from China [19], Bangladesh [20], Nepal [ [6,8,29,30], lincosamides [6,8,[29][30][31], and ansamycins [6,8,29,30] antibiotic agents. Differences in the opinion of medical and veterinary practitioners in antibiotic prescription, observation of ethics and rules in the use of antibiotics, availability or lack of antibiotics and their prices are probable reasons of differences found in the incidence of resistance of S. aureus strains in numerous investigations.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the over than 80% similarity, 36 S. aureus isolates were classi ed in 9 different pro les with 43 to 100% similarities. Isolates No 2,11,20,24,35,5,12,13 and 36 were classi ed in diverse pro les. The lowest similarity (43%) was found for isolates No 20, 11, 2 and 24.…”

Section: Molecular Typing Of S Aureus Strainsmentioning

confidence: 99%

Molecular typing, phenotypic and genotypic assessment of antibiotic resistance and virulence factors amongst the Staphylococcus aureus bacteria isolated from raw chicken meat

Fazel

Karimi

Momtaz

2020

Preprint

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Background: Staphylococcus aureus is an important cause of foodborne diseases due to the consumption of contaminated raw chicken meat. The present research was performed to evaluate the phenotypic and genotypic properties of antibiotic resistance, virulence factor profiles and molecular typing of S. aureus strains isolated from chicken meat. A total of 36 S. aureus strains were isolated from raw chicken meat samples. Phenotypic pattern of antibiotic resistance was assessed by disk diffusion. Distribution of antibiotic resistance and virulence genes was evaluated by PCR. Molecular typing of isolates was perform ed by the ERIC-PCR. Results:Considering the over than 80% similarity, 36 S. aureus isolates were classified in 9 different profiles with 43 to 100% similarities. S. aureus strains showed the highest incidence of resistance against penicillin (100%), tetracycline (91.66%), cephalothin (77.77%), ciprofloxacin (75%), erythromycin (75%), mupirocin (63.88%), clindamycin (61.11%) and trimethoprim/sulfamethoxazole (61.11%). The most commonly detected antibiotic resistance genes amid the S. aureus isolates were mecA (100%), tetK (80.55%), tetM (66.66%), aacA-D (61.11%), msrA (55.55%) and ermA (55.55%).Total distribution of etB, etA, tsst-1, clfA and coa virulence factors amongst the S. aureus strains was 61.11%, 58.33%, 13.88%, 75% and 100%, respectively. Conclusions:Genetic cluster of bacteria affected the antibiotic resistance and virulence characters of S. aureus strains. S. aureus strains with the same ERIC-genetic cluster had similar antibiotic resistance and virulence characters which may show their similar origins. Presence of one or more virulence factors and antibiotic resistance genes amongst the resistant-S. aureus strains signifies an important public health threat rendering the consumption of raw or undercooked chicken meat.

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Characterization, prevalence and antibiogram study of Staphylococcus aureus in poultry

Cited by 30 publications

References 28 publications

Multi–drug resistant Staphylococcus aureus isolated from milk, chicken meat, beef and egg in Bangladesh

Multi–drug resistant Staphylococcus aureus isolated from milk, chicken meat, beef and egg in Bangladesh

Detection of Drug-resistant S. aureus from Poultry Samples Collected from Different Areas of Bangladesh

Molecular typing, phenotypic and genotypic assessment of antibiotic resistance and virulence factors amongst the Staphylococcus aureus bacteria isolated from raw chicken meat

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