Characterization of β-galactosidase from Lactobacillus acidophilus: Stability and kinetic study

Carevic, B Milica; Vukasinovic-Sekulic, S Maja; Banjanac, Katarina; Milivojevic, D Ana; Ćorović, Marija; Bezbradica, Dejan

doi:10.5937/savteh1701005c

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Several models were developed using the various mechanistic sequences of steps shown in Table , with between five and eight kinetic parameters. In this paper, models involving product inhibition will not be considered, because in our previous studies, , the inhibitory effect was not observed.…”

Section: Model and Methodologymentioning

confidence: 99%

Kinetic Model for Galacto-Oligosaccharide Synthesis

Pravilović

Todić

Simović

et al. 2022

Ind. Eng. Chem. Res.

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A new micro-kinetic model is proposed for galacto-oligosaccharide (GOS) synthesis with β-galactosidase from Aspergillus oryzae. Several kinetic models with similar reaction mechanisms, i.e., steps and kinetic constants, have been investigated covering a range of initial lactose (20−40 wt %) and enzyme concentrations (0.5−2 mg/mL). The kinetic parameters were estimated simultaneously for all experimental results using a hybrid genetic algorithm. A detailed analysis of the effect of process parameters on the concentrations of lactose and GOS is performed. The proposed model has a very good quantitative fit of the experimental data, with an average error of 9.34%. More importantly, qualitative trends for the change in the concentration of lactose and GOS are predicted excellently, especially if one takes into account the variation of both concentrations of enzyme and lactose in a range wider than reported in the literature to date. Also, the analysis between the two comparative reactions of transglycosylation and lactose hydrolysis confirmed the positive effect of increasing lactose concentration on the production of GOS. On the other hand, a higher enzyme concentration resulted in a faster production of GOS but with more intensive decomposition at longer reaction times.

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Section: Model and Methodologymentioning

confidence: 99%

Kinetic Model for Galacto-Oligosaccharide Synthesis

Pravilović

Todić

Simović

et al. 2022

Ind. Eng. Chem. Res.

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“…Based on Mahoney (2004), lactose could act as an inducer in βgalactosidase production. A study by Carevic et al (2017) mentioned that β-galactosidase enzyme activity in Lactobacillus acidophilus increased with the addition of 1.48% lactose. Carevic et al (2015) stated that enzyme activity of β-galactosidase increased gradually when the fermentation started and would achieve maximum activity on stationary phase after two days, but then experienced a decrease in activity afterward.…”

Section: Optimum Growth Condition Of Lactobacillus Brevis Lipi13-2-lamentioning

confidence: 99%

“…Carevic et al (2015) also mentioned that the optimum time to achieve maximum βgalactosidase enzyme activity was 48 hours. Based on the research conducted by Carevic et al (2017), there was effect between inoculum size and β-galactosidase enzyme activity. β-galactosidase enzyme activity of Lactobacillus acidophilus increased by the addition of 2.8% inoculum size.…”

Section: Optimum Growth Condition Of Lactobacillus Brevis Lipi13-2-lamentioning

confidence: 99%

Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in β-galactosidase enzyme production

Indah¹,

Rohmatussolihat

Rahayu

et al. 2020

Biodiversitas

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Abstract. Indah AWN, Rohmatussolihat, Rahayu WP, Setyoningrum F, Priadi G, Afianti F. 2020. Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in β-galactosidase enzyme production. Biodiversitas 21: 5403-5407. Deficiency of β-galactosidase enzyme causes lactose to become undigested in gastrointestinal system, therefore the system needs further addition of external β-galactosidase. The sources β-galactosidase enzyme varies from plants, animals, and microorganisms. In industrial applications, microorganisms have become a considered potential source of β-galactosidase. Lactobacillus brevis LIPI13-2-LAB131 had high β-galactosidase enzyme activity, which was 7.93 U/mL. The aim of this research was to optimize the growth condition of L. brevis LIPI13-2-LAB131 in order to produce maximum β-galactosidase enzyme activity. This research consisted of performing optimization processes using design expert 7.0 (DX7) program with response surface methodology (RSM) and partial purification of β-galactosidase enzyme. The results of this research showed that the optimum growth conditions of L. brevis LIPI13-2-LAB131 were in 1.48% lactose level, incubation temperature of 34.91 °C, incubation time of 48.48 hours, and 2.83% inoculum size with desirability value of 0.839. The result of enzyme purification showed that value of β-galactosidase enzyme activity increased up to 22.88 ± 0.29 U/mL with purification yield of 11.65%.

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In Vitro Screening of Indigenous Lactobacillus Isolates for Selecting Organisms with Better Health-Promoting Attributes

Mandal

Bagchi

2018

Appl Biochem Biotechnol

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Lactobacilli have several attributes that provide health benefits to the host. The aim of this study was to screen indigenous lactobacilli from human gut and fermented foods for such attributes as production of β- and α-galactosidase and also their ability to reduce serum cholesterol. Lactobacilli were cultured on MRS broth and β-galactosidase activity was determined using o-nitrophenyl-β-D-galactopyranoside (ONPG) as a substrate. Three isolates Lactobacillus fermentum GPI-3 and L. fermentum GPI-6 and Lactobacillus salivarius GPI-1(S) showed better β-galactosidase activity than the standard strains Lactobacillus rhamnosus GG (LGG) and Lactobacillus plantarum ATCC 8014. The isolates showed variability in assimilating cholesterol during growth. Several isolates showed excellent cholesterol-lowering ability compared to standard strains LGG and L. plantarum ATCC 8014. Isolate L. rhamnosus SCB being the highest acid producer (pH 4.38) also showed the highest cholesterol reduction compared to other strains including standard strains. The ability of these isolates to produce α-galactosidase was also studied and the maximum α-galactosidase activity was found in isolate L. salivarius GPI-1(S) followed by L. fermentum FA-5 and Lactobacillus helveticus FA-7. This study therefore reports Lactobacillus isolates that have superior probiotic properties when compared to the standard strains; hence, they could be considered as potential probiotic strains, which can provide health benefits to the Indian population.

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Characterization of β-galactosidase from Lactobacillus acidophilus: Stability and kinetic study

Cited by 7 publications

References 27 publications

Kinetic Model for Galacto-Oligosaccharide Synthesis

Kinetic Model for Galacto-Oligosaccharide Synthesis

Optimum growth conditions of Lactobacillus brevis LIPI13-2-LAB131 in β-galactosidase enzyme production

In Vitro Screening of Indigenous Lactobacillus Isolates for Selecting Organisms with Better Health-Promoting Attributes

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