Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development

Huang, Huizhe; Wang, Hua; Guo, Fengjin; Du, Xuqin; Ma, Linqiang; Zhao, Liang; Pan, Zhuma; Gui, Hai-bo; Yuan, Taixian; Liu, Xin; Song, Lin; Wang, Yiquan; He, Jie; Liu, Han; Gao, Rui

doi:10.1155/2014/309385

Cited by 6 publications

(6 citation statements)

References 32 publications

(36 reference statements)

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“…In addition to mice, zebrafish are also suitable models for cancer research. In the literature, zebrafish have been used successfully to elucidate the role of pax9 in the development of blood cells [ 41 , 72 ], oro-esophageal squamous epithelium [ 30 ], fin [ 73 ], tooth [ 74 ], and palate [ 75 ].…”

Section: Future Directionsmentioning

confidence: 99%

PAX9 in Cancer Development

Chen

Paiboonrungruang

et al. 2022

IJMS

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Paired box 9 (PAX9) is a transcription factor of the PAX family functioning as both a transcriptional activator and repressor. Its functional roles in the embryonic development of various tissues and organs have been well studied. However, its roles and molecular mechanisms in cancer development are largely unknown. Here, we review the current understanding of PAX9 expression, upstream regulation of PAX9, and PAX9 downstream events in cancer development. Promoter hypermethylation, promoter SNP, microRNA, and inhibition of upstream pathways (e.g., NOTCH) result in PAX9 silencing or downregulation, whereas gene amplification and an epigenetic axis upregulate PAX9 expression. PAX9 may contribute to carcinogenesis through dysregulation of its transcriptional targets and related molecular pathways. In summary, extensive studies on PAX9 in its cellular and tissue contexts are warranted in various cancers, in particular, HNSCC, ESCC, lung cancer, and cervical SCC.

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Section: Future Directionsmentioning

confidence: 99%

PAX9 in Cancer Development

Chen

Paiboonrungruang

et al. 2022

IJMS

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“…Its conserved roles can be demonstrated by similar observations in different species. For example, Pax1 is essential for fin bud development in zebrafish, wing bud development in chick and limb bud development in mouse embryos ( Timmons et al, 1994 ; Hofmann et al, 1998 ; Chen et al, 2014 ). Interestingly, as a transcription factor in the same subfamily with similar protein domains, PAX9 was often investigated in parallel with PAX1.…”

Section: Discussionmentioning

confidence: 99%

“…Embryonic chick wings show morphological defects in the shoulder girdle when Pax1 expression is reduced by ectopic application of signaling molecules (BMP2 and BMP4) ( Hofmann et al, 1998 ), which parallel the defects seen in Pax1 mutant mice ( Timmons et al, 1994 ; Dietrich and Gruss, 1995 ). In zebrafish, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects which could not be phenocopied by pax9 MO and could not be rescued by either pax1a or pax9 overexpression, whereas could be partially rescued by mouse Pax1 mRNA injection ( Liu et al, 2013 ; Chen et al, 2014 ). Loss-of-function of pax1b in zebrafish affects the expression of col2a1 , uncx4.1 , noggin3 and aggrecan , suggesting its roles in chondrocytes differentiation ( Chen et al, 2014 ).…”

Section: Biological Functions Of Pax1 In Embryonic Developmentmentioning

confidence: 99%

“…In zebrafish, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects which could not be phenocopied by pax9 MO and could not be rescued by either pax1a or pax9 overexpression, whereas could be partially rescued by mouse Pax1 mRNA injection ( Liu et al, 2013 ; Chen et al, 2014 ). Loss-of-function of pax1b in zebrafish affects the expression of col2a1 , uncx4.1 , noggin3 and aggrecan , suggesting its roles in chondrocytes differentiation ( Chen et al, 2014 ). While in chick embryos, Pax1 acts synergistically with Pax9, mediating Shh signaling from the notochord and the floor plate, and directly induces Bapx1 (also known as Nkx3.2 ) expression in chondrogenic differentiation of sclerotomal cells ( Rodrigo et al, 2003 ) ( Figure 2 ).…”

Section: Biological Functions Of Pax1 In Embryonic Developmentmentioning

confidence: 99%

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An overview of PAX1: Expression, function and regulation in development and diseases

Kong²,

Jia³

et al. 2022

Front. Cell Dev. Biol.

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Transcription factors play multifaceted roles in embryonic development and diseases. PAX1, a paired-box transcription factor, has been elucidated to play key roles in multiple tissues during embryonic development by extensive studies. Recently, an emerging role of PAX1 in cancers was clarified. Herein, we summarize the expression and functions of PAX1 in skeletal system and thymus development, as well as cancer biology and outline its cellular and molecular modes of action and the association of PAX1 mutation or dysregulation with human diseases, thus providing insights for the molecular basis of congenital diseases and cancers.

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“…Among these are twist , pax1 , pax9 , nkx3.1 and nkx3.2 (Akazawa et al 2000 ; Balling et al 1996 ; Barnes and Firulli 2009 ; Gitelman 1997 ; Herbrand et al 2002 ; Peters et al 1999 ; Provot et al 2006 ; Rainbow et al 2014 ; Tribioli and Lufkin 1999 ). Expression of these markers has been shown to be conserved in the zebrafish sclerotome (Germanguz et al 2007 ; Nornes et al 1996 ; Liu et al 2013 ; Ma et al 2018 ), but only very rarely has their involvement in specification and development of this somitic compartment been investigated using this model organism (Yang et al 2011 ; Ma et al 2018 ; Chen et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

atoh8 expression pattern in early zebrafish embryonic development

Fragale

Divvela

Brand‐Saberi

2021

Histochem Cell Biol

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Atonal homologue 8 (atoh8) is a basic helix-loop-helix transcription factor expressed in a variety of embryonic tissues. While several studies have implicated atoh8 in various developmental pathways in other species, its role in zebrafish development remains uncertain. So far, no studies have dealt with an in-depth in situ analysis of the tissue distribution of atoh8 in embryonic zebrafish. We set out to pinpoint the exact location of atoh8 expression in a detailed spatio-temporal analysis in zebrafish during the first 24 h of development (hpf). To our surprise, we observed transcription from pre-segmentation stages in the paraxial mesoderm and during the segmentation stages in the somitic sclerotome and not—as previously reported—in the myotome. With progressing maturation of the somites, the restriction of atoh8 to the sclerotomal compartment became evident. Double in situ hybridisation with atoh8 and myoD revealed that both genes are expressed in the somites at coinciding developmental stages; however, their domains do not spatially overlap. A second domain of atoh8 expression emerged in the embryonic brain in the developing cerebellum and hindbrain. Here, we observed a specific expression pattern which was again in contrast to the previously published suggestion of atoh8 transcription in neural crest cells. Our findings point towards a possible role of atoh8 in sclerotome, cerebellum and hindbrain development. More importantly, the results of this expression analysis provide new insights into early sclerotome development in zebrafish—a field of research in developmental biology which has not received much attention so far.

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Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development

Cited by 6 publications

References 32 publications

PAX9 in Cancer Development

PAX9 in Cancer Development

An overview of PAX1: Expression, function and regulation in development and diseases

atoh8 expression pattern in early zebrafish embryonic development

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