PAX9 in Cancer Development

Chen, Xiaoxin; Li, Yahui; Paiboonrungruang, Chorlada; Li, Yong; Peters, Heiko; Kist, Ralf; Xiong, Zhaohui

doi:10.3390/ijms23105589

Cited by 11 publications

(7 citation statements)

References 75 publications

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“…We focused on the transcription factor PAX9 on the basis of results from CAGE and GSEA technologies. PAX9 belongs to the paired box family that encodes a group of growth-and development-regulation-related transcription factors [39]. GSEA results also indicated the involvement of PAX9 in head morphogenesis, body morphogenesis, face development, tissue development, and regulation of animal organ morphogenesis.…”

Section: Discussionmentioning

confidence: 91%

PAX9 regulates periodontal ligament stem cell-like differentiation of human induced pluripotent stem cells

Sugiura

Tomokiyo

Hasegawa

et al. 2022

Preprint

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Periodontal ligament stem cells (PDLSCs) play central roles in periodontal ligament (PDL) tissue homeostasis, repair, and regeneration. Previously, we established a protocol to differentiate human induced pluripotent stem cell-derived neural crest-like cells (iNCs) into PDLSC-like cells (iPDLSCs) using human PDL cell-derived extracellular matrix (ECM). However it remained unclear what factors principally regulate the differentiation of iNCs into iPDLSCs. In this study, we aimed to identify the transcription factor regulating production of human PDL cell-derived ECM, which is responsible for the generation of iPDLSCs. We cultured iNCs on two human PDL cell lines (HPDLC-3S and HPDLC-3U) and human dermal fibroblasts (HDF). iNCs cultured on HPDLC-3U showed higher iPDLSC-associated gene expression and mesenchymal differentiation capacity than cells cultured on HDF or HPDLC-3S. The transcription factor PAX9 was highly expressed in HPDLC-3U compared with HDF and HPDLC-3S. iNCs cultured on siPAX9-transfected HPDLC-3U displayed downregulation of iPDLSC-associated marker expression and adipocytic differentiation capacity relative to controls. Our findings suggest that PAX9 is one of the transcription factors regulating ECM production in human PDL cells, which is responsible for the differentiation of iNCs into iPDLSCs.

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Section: Discussionmentioning

confidence: 91%

PAX9 regulates periodontal ligament stem cell-like differentiation of human induced pluripotent stem cells

Sugiura

Tomokiyo

Hasegawa

et al. 2022

Preprint

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“…For example, recent studies have shown that PAX8 can regulate tumor-stroma interaction, cell adhesion, proliferation, and migration, and mutations in PAX8 are thought to be associated with the development and progression of 90% of ovarian cancers [28,29]. PAX9 gene mutation, ampli cation and deletion can promote the development of lung adenocarcinoma, squamous cell carcinoma, cervical cancer, ovarian cancer and breast cancer [30]. PAX8 is highly expressed in cervical cancer, and PAX8 transcripts are upregulated in cervical cancer samples, suggesting a correlation with cervical cancer development [31], and PAX8 expression is also upregulated in uterine adenocarcinoma, squamous cell carcinoma, endometrioid adenocarcinoma, and adenosquamous carcinoma [32][33][34][35][36][37][38][39][40][41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance and immunologic features of the paired-box (PAXs) family: a pan-cancer multi- omics analysis

Zhou,

Qi,

Xia

et al. 2024

Preprint

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Objective The PAX genes, comprising a family of nine clearly defined paired-box transcription factors, are associated with the onset and progression of certain tumors. Even so, no extensive systematic investigation toward the contribution of PAX genes in pan-cancer has been implemented. Methods The development and modulation of the PAX gene family in pan tumor and its correlations with prognosis, tumor mutation burden (TMB), microsatellite instability (MSI), immunological subtypes, immune checkpoint genes, tumour stemness, tumor microenvironment, chemotherapeutics sensitivity, and effectiveness of immunotherapy were explored by bioinformatics analysis, based on multi-omics data from TCGA, GEO, cBioPortal, and TIMER database. Results We observed the significant correlations between the regulation of particular PAX family members in pan tumor and the survival prognosis and tumor stage of patients, TMB, MSI, stemness scores, immune cells infiltration, etc. The PAX gene family exhibited some degree of heterogeneity in different cancers in terms of the above mentioned findings. It has also been revealed in the present multiple omics study that the expression for most of the PAX family members, including PAX1/3/5/8/9, is significantly correlated with copy number variation. Moreover, we also found that several PAX family members were clearly associated with expression of immune checkpoint genes, the sensitivity to chemotherapy agents, and anti-PD-L1/PD-1 immunotherapy. Furthermore, the invading immune evaluation in bladder tumors displayed substantial correlations between PAX gene variations in copy number or substitution levels and the extent of multiple immune cell infiltration. In addition, the mRNA and amino acid manifestations of PAX8 in BLCA were validated using real-time PCR (RT-PCR) and the Human Protein Atlas (HPA). Conclusion In summary, our findings highlight the importance of PAX family genes in predictions of various tumor types, as evidenced by multiple datasets and identified PAX-associated genes that could be used as targets for therapies. These results suggest that PAX family related genes can be used as potential prognostic markers for cancer. It represents a systematic analysis of the further function of PAX family genes, which can provide new ideas for the prognosis and treatment of various cancers.

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“…We focused on the transcription factor PAX9 based on results from CAGE and GSEA technologies. PAX9 belongs to the paired box family that encodes a group of growth- and development-regulation-related transcription factors [ 49 ]. GSEA results also indicated the involvement of PAX9 in head morphogenesis, body morphogenesis, face development, tissue development, and regulation of animal organ morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix

et al. 2022

View full text Add to dashboard Cite

Periodontal ligament stem cells (PDLSCs) play central roles in periodontal ligament (PDL) tissue homeostasis, repair, and regeneration. Previously, we established a protocol to differentiate human-induced pluripotent stem cell-derived neural crest-like cells (iNCs) into PDLSC-like cells (iPDLSCs) using human PDL cell-derived extracellular matrix (ECM). However, it remained unclear what factors principally regulate the differentiation of iNCs into iPDLSCs. In this study, we aimed to identify the transcription factor regulating production of human PDL cell-derived ECM, which is responsible for the generation of iPDLSCs. We cultured iNCs on ECMs of two human PDL cell lines (HPDLC-3S and HPDLC-3U) and of human dermal fibroblasts (HDF). iNCs cultured on HPDLC-3U demonstrated higher iPDLSC-associated gene expression and mesenchymal differentiation capacity than cells cultured on HDF or HPDLC-3S. The transcription factor PAX9 was highly expressed in HPDLC-3U compared with HDF and HPDLC-3S. iNCs cultured on siPAX9-transfected HPDLC-3U displayed downregulation of iPDLSC-associated marker expression and adipocytic differentiation capacity relative to controls. Our findings suggest that PAX9 is one of the transcription factors regulating ECM production in human PDL cells, which is responsible for the differentiation of iNCs into iPDLSCs.

show abstract

PAX9 in Cancer Development

Cited by 11 publications

References 75 publications

PAX9 regulates periodontal ligament stem cell-like differentiation of human induced pluripotent stem cells

PAX9 regulates periodontal ligament stem cell-like differentiation of human induced pluripotent stem cells

Prognostic significance and immunologic features of the paired-box (PAXs) family: a pan-cancer multi- omics analysis

PAX9 Is Involved in Periodontal Ligament Stem Cell-like Differentiation of Human-Induced Pluripotent Stem Cells by Regulating Extracellular Matrix

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