Characterization of Yarrowia lipolytica and related species for citric acid production from glycerol

Levinson, William E.; Kurtzman, Cletus P.; Kuo, Tsung Min

doi:10.1016/j.enzmictec.2007.02.005

Cited by 107 publications

(68 citation statements)

References 12 publications

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“…6, iv). In these latter conditions, Y. lipolytica produces citric acid (Levinson et al, 2007) but other oleaginous yeasts have never been reported to behave this way.…”

Section: Cultivation Condition Of Oleaginous Yeastsmentioning

confidence: 99%

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Rossi¹,

Amaretti²,

Raimondi³

et al. 2011

Biodiesel - Feedstocks and Processing Technologies

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“…6, iv). In these latter conditions, Y. lipolytica produces citric acid (Levinson et al, 2007) but other oleaginous yeasts have never been reported to behave this way.…”

Section: Cultivation Condition Of Oleaginous Yeastsmentioning

confidence: 99%

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Rossi¹,

Amaretti²,

Raimondi³

et al. 2011

Biodiesel - Feedstocks and Processing Technologies

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“…In the literature, it has been reported that in addition to studies in which pure glycerol is used as a substrate, there are other studies in which crude glycerol is used as a byproduct in the production of biodiesel and soap [2,3,19,22,28].…”

Section: Discussionmentioning

confidence: 99%

“…The citric acid and isocitric acid produting medium consisted of the following (g/L): (NH 4 3 • 6H 2 O 0.6; thiamine 0.0003. Also the medium including 50 and 100 (g/L) concentrations of glucose, glycerol and sunflower oil as a carbon sources was investigated.…”

Section: Organic Acid Producing Media and Analytical Methodsmentioning

confidence: 99%

“…With a global annual production exceeding 1.7 million tons, citric acid (CA) ranks first among the organic acid synthesized by microorganisms [2] and its production volume is second only to industrial ethanol as a fermentation product [3]. Citric acid (2-hydroxy-1,2,3-propanetricarboxylic acid), is a natural component and common metabolite of plants and animals and is the most versatile and widely used organic acid in foods, beverages, and pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

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Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Uçar¹,

Çelik²,

Akpınar³

et al. 2014

Turk J Bioch

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Objective: The goal of this study was to determine the influence of various carbon sources on the citric and isocitric acid production by various Yarrowia lipolytica strains. Methods:The yeasts used in our study were first investigated for organic acid production using screening media. Then, the effect of several complex carbon sources on the citric and isocitric acid production of selected yeast strains was investigated. The amount of citric and isocitric acid production was determined via enzymatic reactions. Results: In this study, 22 Y. lipolytica strains were investigated for the organic acid production. Among these strains, 2 strains (TEM YL 3 and TEM YL 20) were found to be highest organicacid producer. Taken all results together, the highest amounts of citric acid (66.2 g/L for TEM YL 3, 50.0 g/L for TEM YL 20) were observed in the production medium containing sunflower oil. Conclusion: Citric acid consumption, and thus, the need for it are constantly rising in our country, which imports citric acid. Therefore, in order to meet this need, further studies which will yield to the maximum citric acid production should be performed by utilizing waste carbon sources and by using new low-cost but high citric acid-producing strains. Key Words: Organic acid production, citric acid, isocitric acid, Yarrowia lipolytica. Conflict of Interest: Authors do not have any conflict of interest. ÖZETAmaç: Bu çalışmada, Yarrowia lipolytica suşları tarafından sitrik ve izositrik asit üretimi üzerine çeşitli karbon kaynaklarının etkisinin saptanması amaçlanmıştır. Metod: İlk olarak çalışmamızda kullandığımız mayalar, organik asit üretimleri açısından tarama besiyerlerinde test edilmişlerdir. Daha sonra, bu maya strainleri tarafından üretilen sitrik ve izositrik asit üretimi üzerine farklı karbon kaynaklarının etkisi araştırılmıştır. Sitrik ve izositrik asit üretim miktarları enzimatik test kitleri kullanılarak saptanmıştır. Bulgular: Bu çalışmada, organik asit üretimi açısından 22 Y. lipolytica suşu incelenmiştir. Bu 22 maya suşu arasından iki suşun (TEM YL 3 ve TEM YL 20), en yüksek organik asit üretici maya suşları olduğu bulunmuştur. Bütün sonuçlar değerlendirildiğinde, en yüksek sitrik asit üretimi (TEM YL 3 için 66.2 g/L, TEM YL 20 için 50.0 g/L) ayçiçek yağı içeren üretim ortamında saptanmıştır. Sonuç: Özellikle sitrik asit ithalatı yapılan ülkemizde, sitrik asit tüketimi ve ona olan ihtiyaç sürekli olarak artmaktadır. Atık karbon kaynaklarının değerlendirilmesi ve maliyeti düşük ancak verimi yüksek sitrik asit üreten yeni suşların kullanılarak, maksimum sitrik asit verimini sağlayacak daha ileri çalışmalara ihtiyaç duyulmaktadır. Material and Methods Microorganisms and growth conditionsDomestic Y. lipolytica strains TEM YL 3 and TEM YL 20 had been isolated from cheese [14] and identified with phenotypic and genotypic approach [14,15]. Initially yeast cells were grown aerobically at 27°C in a liquid YPD medium including (1.0 % yeast extract, 2.0 % peptone, and 2.0 % glucose). Screening of total organic acid pro...

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“…One of the most promising applications for the use of glycerol is its bioconversion to value-added compounds through microbial fermentation. Besides high-value surfactants and enzymes, biopolymers can also be produced by microorganisms from glycerol [8].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

Castro¹

2014

J Microb Biochem Technol

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The industrial conversion of renewable resources into bio-based materials has been increasingly receiving attention from an economic and, in particular, an environmental point of view. Among these bioresources, glycerol represents one of the most important base materials for the manufacturing of bulk and higher-valued products. This is mainly based on the increasing amounts of waste glycerol due to the rising production of biodiesel and other oleochemicals, as well as on the applicability of glycerol as a water-soluble carbon source for a wide range of microbial productions. Polyhydroxyalkanoates (PHAs) are biodegradable and environmentally friendly biopolymers, and the major contributor to PHA production is the substrate cost, therefore it is desirable to produce PHA from waste residues like biodiesel byproducts. Since soil ecosystems have a rich, but still insufficiently studied microbial flora, the ability of bacteria isolated from Finnish soils and sediments were investigated for the production of valueadded materials such as Polyhydroxyalkanoates based on pure and waste glycerol as carbon source. 1 out of 40 isolated strains was selected for further studies based on ability to produce PHA on mineral medium and identified as Halomonas sp SA8 with up to 56% of Polyhydroxyalkanoate accumulation. The produced biopolymer was recovered and identified as PHB homopolymer.

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Characterization of Yarrowia lipolytica and related species for citric acid production from glycerol

Cited by 107 publications

References 12 publications

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Getting Lipids for Biodiesel Production from Oleaginous Fungi

Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

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