Characterization of Xenorhabdus and Photorhabdus bacteria by Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR)

The accurate reliable detection and identification of microorganisms in food is critical to public safety. Consequently, it is extremely important to develop rapid and inexpensive methods for the detection of food microorganisms in order to minimize or even replace the traditional analysis methods that are expensive and time-consuming. In this study, the potential of mid-infrared spectroscopy was evaluated, for the first time, to detect changes in colony forming units of microorganisms in freshly cut ham along the time. A partial least squares regression model was performed and a good linear relationship was obtained between spectra information and microbial load. It was concluded that infrared spectroscopy easily and quickly allows the separation of ham samples according to their microbial content and could be used to predict the microbial concentration from the spectra, using the fingerprint region (1,200-950 cm −1 ), without sample preparation or handling. PRACTICAL APPLICATIONSAs it is essential to avoid infections caused by foodborne bacteria, it is important to develop a rapid, low cost and easy to perform technique to face the increasing demands of the food industry. Mid-infrared spectroscopy, coupled to multivariate analysis, has potential to be used as a first-screening approach and to assess the microbial concentration in ham samples, avoiding the traditional plating methods that are time-consuming.

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“…), overnight drying of colonies obtained from plates (San‐Blas et al . ) or colonies grown in liquid medium (Alexandrakis et al . ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Potential of Mid‐Infrared Spectroscopy to Assess the Microbiological Quality of Ham

Moreirinha

Nunes

Barros

et al. 2015

Journal of Food Safety

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“…Previously to Boudau's work, an attempt to discriminate these two bacterial groups (sulphate and thiosulphate reducing bacteria) in the context of bio-corrosion processes, did not report better results. [17] More recently, San-Blas and coworkers [18] successfully discriminate three genera of the Enterobactereaceae family: Xenorhabdus, Photorhabdus, and Escherichia. These genera are associated with insect lethal septicemia.…”

Section: Bacterial Typing At the Genus Levelmentioning

confidence: 99%

“…Sulphate and thiosulphate reducing bacteria Genus FTIRS, FTIRS-ATR HCA, PCA [16,17] Alicylobacillus Genus FTIRS PCA, SIMCA [20] Strain FTIRS-HATR LDA, PCA [75] Photorhabdus Genus FTIRS-ATR HCA, PCA [18] Xenorhabdus Genus FTIRS-ATR HCA, PCA [18] Arcanobacterium Genus FTIRS ANN [19] Actinomyces Genus FTIRS ANN [19] Trueperella Genus FTIRS ANN [19] Species FTIRS ANN [19] Bacillus Genus FTIRS PCA, SIMCA [20] Species FTIRS, FTIRS-ATR, Diffuse Reflectance-FTIRS PCA, HCA, DFA, CVA, SIMCA [26][27][28][29][30]49,50,54,55] Vegetative/Sporulated FTIRS PCA, CART [83] Escherichia Genus FTIRS-ATR HCA, PCA [18] Species FTIRS, FTIRS-ATR HCA, ANN, PCA, SIMCA, CVA [49][50][51][52][53][54]86,92] Escherichia coli Sequence type FTIRS, FTIRS-ATR PC-DFA, PLSDA, HCA, SIMCA [67,68] Strain FTIRS PCA, SIMCA, CVA [49,50,52,73] MLVA profile FTIRS HCA, CVA [72] Listeria spp. Species FTIRS, FTIRS-ATR, FTIR microspectroscopy CVA, ANN, SCDA, PLSRDA, PCA [5,[21][22][23][24][25]…”

Section: Bacteria Discrimination Level Infrared Technique Chemometricmentioning

confidence: 99%

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

Quintelas

Ferreira

Lopes

et al. 2017

Biotechnology Journal

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The sustained emergence of new declared bacterial species makes typing a continuous challenge for microbiologists. Molecular biology techniques have a very significant role in the context of bacterial typing, but they are often very laborious, time consuming, and eventually fail when dealing with very closely related species. Spectroscopic-based techniques appear in some situations as a viable alternative to molecular methods with advantages in terms of analysis time and cost. Infrared and mass spectrometry are among the most exploited techniques in this context: particularly, infrared spectroscopy emerged as a very promising method with multiple reported successful applications. This article presents a systematic review on infrared spectroscopy applications for bacterial typing, highlighting fundamental aspects of infrared spectroscopy, a detailed literature review (covering different taxonomic levels and bacterial species), advantages, and limitations of the technique over molecular biology methods and a comparison with other competing spectroscopic techniques such as MALDI-TOF MS, Raman, and intrinsic fluorescence. Infrared spectroscopy possesses a high potential for bacterial typing at distinct taxonomic levels and worthy of further developments and systematization. The development of databases appears fundamental toward the establishment of infrared spectroscopy as a viable method for bacterial typing.

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“…The nematode spectra also showed a series of bands representative of lipids, proteins and sugars that were speciesdependent. Also, the symbiotic bacteria of the above mentioned nematodes were compared with Escherichia coli Migula (Enterobacteriales: Enterobacteriaceae) and again the separation of spectra was possible between them (San-Blas, Cubillán, Guerra, Portillo, & Esteves, 2012). Ongoing experiments are currently elucidating differences on the lipid composition of EPNs reared in different hosts and studies on the effect of the concentration of trehalose produced by the EPNs in stress conditions on their virulence and pathogenicity.…”

Section: Molecular and Biochemical Studiesmentioning

confidence: 99%

Nematode Pathogenesis of Insects and Other Pests

Campos‐Herrera¹

2015

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Characterization of Xenorhabdus and Photorhabdus bacteria by Fourier transform mid-infrared spectroscopy with attenuated total reflection (FT-IR/ATR)

Cited by 26 publications

References 24 publications

Evaluation of the Potential of Mid‐Infrared Spectroscopy to Assess the Microbiological Quality of Ham

Evaluation of the Potential of Mid‐Infrared Spectroscopy to Assess the Microbiological Quality of Ham

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

Nematode Pathogenesis of Insects and Other Pests

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