Characterization of vegetative vigor of two doubled-haploid wheat populations

Khumalo, Thobeka Philile; Dube, Ernest; Tsilo, Toi J.

doi:10.1080/15427528.2021.1970675

Cited by 5 publications

(4 citation statements)

References 65 publications

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“…The generation of homozygous individuals by haploid doubling has become a useful and efficient tool in the breeding of plants such as maize [27,28], wheat [29,30] and rice [31,32]. The main reasons for using DHs in breeding programs are: (1) obtaining pure lines in a single generation, (2) fixing desirable genotypic combinations, (3) increasing selection efficiency, and (4) reducing sample size for selection.…”

Section: Importance Of Doubled Haploidsmentioning

confidence: 99%

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Marín-Montes

Rodríguez-Pérez

Robledo-Paz

et al. 2022

Plants

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The generation of new hybrid varieties of tomato (Solanum lycopersicum L.) is the most widely used breeding method for this species and requires at least seven self-fertilization cycles to generate stable parent lines. The development of doubled haploids aims at obtaining completely homozygous lines in a single generation, although, to date, routine commercial application has not been possible in this species. In contrast, obtaining doubled haploid lines via gynogenesis has been successfully implemented in recalcitrant crops such as melon, cucumber, pumpkin, loquat and walnut. This review provides an overview of the requirements and advantages of gynogenesis as an inducer of haploidy in different agricultural crops, with the purpose of assessing the potential for its application in tomato breeding. Successful cases of gynogenesis variants involving in vitro culture of unfertilized ovules, use of 60Co-irradiated pollen, in vivo haploid inducers and wide hybridization are presented, suggesting that these methodologies could be implemented in tomato breeding programs to obtain doubled haploids.

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Section: Importance Of Doubled Haploidsmentioning

confidence: 99%

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Marín-Montes

Rodríguez-Pérez

Robledo-Paz

et al. 2022

Plants

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“…Two DH wheat populations (n=210) derived from two crosses of Tugela-Dn × Elands (Lephuthing et al, 2021) and Elands × Flamink (Khumalo et al, 2021) 1.…”

Section: Plant Material Study Area and Experimental Designmentioning

confidence: 99%

“…Elands is a facultative cultivar that was released for dry-land wheat production in 1998. The cultivar has high yield potential, excellent tolerance to PHS and exceptional bread-making quality, which makes it a quality standard in the South African wheat industry (Khumalo et al, 2021). Flamink is a winter wheat cultivar with high yield potential, however, exhibits a reduced level of tolerance to PHS.…”

Section: Plant Material Study Area and Experimental Designmentioning

confidence: 99%

“…Flamink is a winter wheat cultivar with high yield potential, however, exhibits a reduced level of tolerance to PHS. An augmented design was used in all environments as described in Khumalo et al (2021). Commercial production and agronomic practices were followed as recommended for the specific production region.…”

Section: Plant Material Study Area and Experimental Designmentioning

confidence: 99%

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Genomic Regions Influencing Preharvest Sprouting Tolerance in Two Doubled-Haploid Wheat Populations (Triticum aestivum L.)

Khumalo¹,

Hlongoane²,

Tsilo³

2021

Preprint

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The current and projected climate change that is represented by increasing temperatures, humidity levels and irregular rainfall patterns, promotes the occurrence of preharvest sprouting (PHS) in wheat. PHS results in significant economic losses, globally, which necessitates the need for high-yielding cultivars with increased PHS tolerance, hence this study was conducted. The current study evaluated two doubled-haploid (DH) wheat populations of Tugela-Dn × Elands and Elands × Flamink across six environments in the Free State Province of South Africa to select genotypes with increased PHS tolerance and further map the underlying loci. Significant effects of DH lines (194) and environments (6) were observed for PHS tolerance. The results of this study validate previous findings that PHS is only expressed when environmental conditions are conducive. Quantitative trait loci (QTL) mapping using single nucleotide polymorphism (SNP) and silicoDArT markers revealed three additive QTL with major effects on chromosomes 5B and 7B, and these QTL were detected more than once, when conditions were favourable. These QTL explained a phenotypic variation (PVE) varying between 10.08% and 20.30% (LOD = 2.73 – 3.11). About 16.50% of DH lines performed to the level of Elands (the PHS-tolerant parent) and are recommended for further selection in a pre-breeding or breeding programme. The findings of the study are expected to facilitate the on-going breeding efforts for PHS tolerance in winter wheat.

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Estimation of the efficiency of developing regenerative winter bread wheat lines in anther culture <i>in vitro</i>

Chertkova

Kalinina

Dontsova

et al. 2023

Zernovoe hozâjstvo Rossii

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The efficiency of the anther culture method in vitro largely depends on the responsiveness of varieties and hybrids to the induction of androgenesis. The purpose of the current study was to estimate the efficiency of haploproduction of regenerated lines (R0) of winter bread wheat developed by anther culture in vitro and grown in a greenhouse. To achieve this goal, there was set a task to conduct a morphometric estimation of the developed heads (R0) to identify their fertility level. The study was carried out in the laboratory of cell breeding of the FSBSI Agricultural Research Center “Donskoy” in 2021–2022. The objects of the study were 7 hybrid combinations of winter wheat, which were responsive to androgenesis in vitro in the culture of isolated anthers. As a result of induced androgenesis on an artificial nutrient medium, there were identified 333 neoplasms, represented by calluses, embryoids and embryogenic complexes. Green regenerated plants were vernalized in test tubes, cloned, diloidized, then planted into soil. There were obtained 13 haploid lines, after diploidization only 34 plants survived. The highest haploproductive capacity was established in the hybrid combination F3 ‘Rostovchanka 7xBezostaya 100’. In this combination, there were formed 8 green regenerants, 21 plants of which were developed together with clones, 18 of which survived. The cytometric estimation has shown, that 25 dihaploid (2n = 42) and 16 haploid (n = 21) wheat plants have been identified by hybrid combinations, including 13 dihaploids from the combination ‘Rostovchanka 7xBezostaya 100’. As a result of the structural analysis and morphometric estimation of the head there has been found a different fertility degree of androgenic regenerated plants. There have been established prospects of using the dihaploid line ‘79.2.1.2’ in further breeding work based on the hybrid combination F3 ‘Rostovchanka 7xBezostaya 100’ with the highest values of such yield elements as ‘number of heads’, ‘number and weight of grains per head’.

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Characterization of vegetative vigor of two doubled-haploid wheat populations

Cited by 5 publications

References 65 publications

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Genomic Regions Influencing Preharvest Sprouting Tolerance in Two Doubled-Haploid Wheat Populations (Triticum aestivum L.)

Estimation of the efficiency of developing regenerative winter bread wheat lines in anther culture <i>in vitro</i>

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