Estimation of the efficiency of developing regenerative winter bread wheat lines in anther culture <i>in vitro</i>

Abstract

The efficiency of the anther culture method in vitro largely depends on the responsiveness of varieties and hybrids to the induction of androgenesis. The purpose of the current study was to estimate the efficiency of haploproduction of regenerated lines (R0) of winter bread wheat developed by anther culture in vitro and grown in a greenhouse. To achieve this goal, there was set a task to conduct a morphometric estimation of the developed heads (R0) to identify their fertility level. The study was carried out in the laboratory of cell breeding of the FSBSI Agricultural Research Center “Donskoy” in 2021–2022. The objects of the study were 7 hybrid combinations of winter wheat, which were responsive to androgenesis in vitro in the culture of isolated anthers. As a result of induced androgenesis on an artificial nutrient medium, there were identified 333 neoplasms, represented by calluses, embryoids and embryogenic complexes. Green regenerated plants were vernalized in test tubes, cloned, diloidized, then planted into soil. There were obtained 13 haploid lines, after diploidization only 34 plants survived. The highest haploproductive capacity was established in the hybrid combination F3 ‘Rostovchanka 7xBezostaya 100’. In this combination, there were formed 8 green regenerants, 21 plants of which were developed together with clones, 18 of which survived. The cytometric estimation has shown, that 25 dihaploid (2n = 42) and 16 haploid (n = 21) wheat plants have been identified by hybrid combinations, including 13 dihaploids from the combination ‘Rostovchanka 7xBezostaya 100’. As a result of the structural analysis and morphometric estimation of the head there has been found a different fertility degree of androgenic regenerated plants. There have been established prospects of using the dihaploid line ‘79.2.1.2’ in further breeding work based on the hybrid combination F3 ‘Rostovchanka 7xBezostaya 100’ with the highest values of such yield elements as ‘number of heads’, ‘number and weight of grains per head’.