Characterization of two TERMINAL FLOWER1 homologs PgTFL1 and PgCENa from pomegranate (Punica granatum L.)

Patil, H. B.; Chaurasia, Akhilesh K.; Azeez, Abdul; Krishna, Bal; Subramaniam, V. R.; Sane, Aniruddha P.; Sane, P. V.

doi:10.1093/treephys/tpx154

Cited by 14 publications

(18 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been found that TFL1 homologues in different plants are relatively conserved in function, which promotes vegetative growth and delays flowering, such as in Arabidopsis (Hanano and Goto, 2011), rice (Nakagawa et al, 2002), chrysanthemum (Higuchi and Hisamatsu, 2015), dogwood (Liu et al, 2016), apple (Flachowsky et al, 2012), black cherry (Wang and Pijut, 2013), and pomegranate (Patil et al, 2018). Furthermore, ectopic expression of EjTFL1s in Arabidopsis has multiple effects, including modified plant height, branching, and inflorescence, silique and cauline leaf morphology ( Figures 5G,H and Supplementary Figure S3).…”

Section: Discussionmentioning

confidence: 99%

EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

et al. 2020

View full text Add to dashboard Cite

TERMINAL FLOWER1 (TFL1), a key factor belonging to the phosphatidyl ethanolaminebinding protein (PEBP) family, controls flowering time and inflorescence architecture in some plants. However, the role of TFL1 in loquat remains unknown. In this study, we cloned two TFL1-like genes (EjTFL1-1 and EjTFL1-2) with conserved deduced amino acid sequences from cultivated loquat (Eriobotrya japonica Lindl.). First, we determined that flower bud differentiation occurs at the end of June and early July, and then comprehensively analyzed the temporal and spatial expression patterns of these EjTFL1s during loquat growth and development. We observed the contrasting expression trends for EjTFL1s and EjAP1s (APETALA 1) in shoot apices, and EjTFL1s were mainly expressed in young tissues. In addition, short-day and exogenous GA 3 treatments promoted the expression of EjTFL1s, and no flower bud differentiation was observed after these treatments in loquat. Moreover, EjTFL1s were localized to the cytoplasm and nucleus, and both interacted with another flowering transcription factor, EjFD, in the nucleus, and EjTFL1s-EjFD complex significantly repressed the promoter activity of EjAP1-1. The two EjTFL1s were overexpressed in wild-type Arabidopsis thaliana Col-0, which delayed flowering time, promoted stem elongation, increased the number of branches, and also affected flower and silique phenotypes. In conclusion, our results suggested that EjTFL1-1 and EjTFL1-2 do not show the same pattern of expression whereas both are able of inhibiting flower bud differentiation and promoting vegetative growth in loquat by integrating GA 3 and photoperiod signals. These findings provide useful clues for analyzing the flowering regulatory network of loquat and provide meaningful references for flowering regulation research of other woody fruit trees.

show abstract

Section: Discussionmentioning

confidence: 99%

EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Considered as the florigen agent, FT activates the flowering pathway, whereas TFL1 represses flowering and is responsible for the maintenance of the inflorescence meristem. The FT/TFL1 balance modulates the plant architecture because both proteins are involved in the control of the indeterminate versus determinate plant growth habit, which is essentially based on the production pattern of vegetative versus reproductive organs by the apical meristem (Matsoukas et al., 2012; Xu et al., 2012; Jaeger et al., 2013; Nakano et al., 2015; Patil et al., 2017).…”

Section: Effects Of Ft/tfl1 Balance In Annual Plants: Arabidopsismentioning

confidence: 99%

FT/TFL1: Calibrating Plant Architecture

2019

View full text Add to dashboard Cite

There is a very large diversity in plant architecture in nature. Over the past few years, novel theoretical concepts and analytical methods have emerged as powerful tools to understand important aspects of plant architecture. Plant architecture depends on the relative arrangement of three types of organs: leaves, shoots, and flowers. During plant development, the architecture is modulated by the balance of two homologous proteins: FLOWERING LOCUS T (FT) and TERMINAL FLOWER 1 (TFL1). The FT/TFL1 balance defines the plant growth habit as indeterminate or determinate by modulating the pattern of formation of vegetative and reproductive structures in the apical and axillary meristems. Here, we present a summarized review of plant architecture and primarily focus on the FT/TFL1 balance and its effect on plant form and development. We also propose passion fruit as a suitable model plant to study the effect of FT/TFL1 genes on plant architecture.

show abstract

“…Thus, different plant species have developed their optimal approaches for the regulation of flowering time with environmental cues [ 15 ]. Two significant phosphatidylethanolamine-binding protein (PEBP) genes controlling these pathways are FT ( FLOWERING LOCUS T ) and TFL1 ( TERMINAL FLOWER 1 ) [ 17 , 18 , 19 ]. TFL1 plays a key role in suppressing flowering by antagonizing the function of the FT protein, which is responsible for the promotion of flowering [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Haider

Gao

2020

Genes

View full text Add to dashboard Cite

Chrysanthemum (Chrysanthemum x morifolium Ramat.) cultivar Jinba is a distinctive short-day chrysanthemum that can be exploited as a model organism for studying the molecular mechanism of flowering. The commercial value of Jinba can be increased in global flower markets by developing its proper regeneration and genetic transformation system. By addressing typical problems associated with Agrobacterium-mediated transformation in chrysanthemum, that is, low transformation efficiency and high cultivar specificity, we designed an efficient, stable transformation system. Here, we identify the features that significantly affect the genetic transformation of Jinba and standardize its transformation protocol by using CmTFL1a as a transgene. The appropriate concentrations of various antibiotics (kanamycin, meropenem and carbenicillin) and growth regulators (6-BA, 2,4-D and NAA) for the genetic transformation were determined to check their effects on in vitro plant regeneration from leaf segments of Jinba; thus, the transformation protocol was standardized through Agrobacterium tumefaciens (EHA105). In addition, the presence of the transgene and its stable expression in CmTFL1a transgenic plants were confirmed by polymerase chain reaction (PCR) analysis. The CmTFL1a transgene constitutively expressed in the transgenic plants was highly expressed in shoot apices as compared to stem and leaves. Overexpression of CmTFL1a led to a delay in transition to the reproductive phase and significantly affected plant morphology. This study will help to understand the biological phenomenon of TFL1 homolog in chrysanthemum. Moreover, our findings can explore innovative possibilities for genetic engineering and breeding of other chrysanthemum cultivars.

show abstract

Characterization of two TERMINAL FLOWER1 homologs PgTFL1 and PgCENa from pomegranate (Punica granatum L.)

Cited by 14 publications

References 66 publications

EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

FT/TFL1: Calibrating Plant Architecture

Standardized Genetic Transformation Protocol for Chrysanthemum cv. ‘Jinba’ with TERMINAL FLOWER 1 Homolog CmTFL1a

Contact Info

Product

Resources

About