Characterization of Two Spontaneously Generated Human Müller Cell Lines from Donors with Type 1 and Type 2 Diabetes

Abstract

HMCLs can be used as a model to improve the understanding of Müller cells in the context of chronic diabetes.

Comparison between the Gene Expression Profile of Human Müller Cells and Two Spontaneous Müller Cell Lines

“…Moreover, only one tag corresponding to the glial fibrillary acidic protein (GFAP) was found in the NHMC library, which is in good agreement with studies indicating that GFAP is normally expressed at low levels in Müller cells. [32][33][34] Compared to NHMCs, a lower expression of VEGF was found in HMCLs, which is consistent with previous RT-PCR analyses with these cells 22 as well as with Müller cells from diabetic rats. 35 Additional proteins known to be expressed by Müller cells such as CD 44 36 56 ; and decorin 57 were found in the NHMC library, as can be seen in Table 4.…”

“…The characterization of the two novel human Müller cell lines (HMCL-I and -II) 22 has shown only a few differences between HMCLs and NHMCs by RT-PCR analyses, but large differences in growth rate and proliferation. 22 SAGE libraries were thus prepared with mRNA from NHMC, HMCL-I, and HMCL-II, to determine the gene expression profile of each of these cell types and genes differentially expressed between NHMCs and HMCLs.…”

“…22 SAGE libraries were thus prepared with mRNA from NHMC, HMCL-I, and HMCL-II, to determine the gene expression profile of each of these cell types and genes differentially expressed between NHMCs and HMCLs.…”

“…To our knowledge, no one has prepared a SAGE library of human Müller cells. In the present study, we used the SAGE method to determine the gene expression profile of normal human Müller cells (NHMCs) and two previously characterized human Müller cell lines (HMCLs) 22 generated from donors with type 1 or type…”

Characterization of a Spontaneously Immortalized Murine Müller Glial Cell Line QMMuC-1

“…Müller glial cell lines, derived from rodents [14][15][16][17] or humans 18 have been reported. Although they all express Müller glial markers, each cell line has its own characteristics and is used to study different functions of Müller glia.…”