Characterization of two Bacillus thuringiensis isolates from South Brazil and their toxicity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Bobrowski, Vera Lúcia; Pasquali, Giancarlo; Bodanese‐Zanettini, Maria Helena; Pinto, Laura Massochin Nunes; Fiúza, Lidia Mariana

doi:10.1016/s1049-9644(02)00055-5

Cited by 26 publications

(18 citation statements)

References 27 publications

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“…The occurrence of the latter type of crystals in more toxic strains was confirmed by several previous studies (Ammouneh et al, 2011). Even though, the correlation between the two facts is not clear as it was noted by Bobrowski et al (2002).…”

Section: Characterization Of Blb 250supporting

confidence: 64%

A promising HD133-like strain of Bacillus thuringiensis with dual efficiency to the two Lepidopteran pests: Spodoptera littoralis (Noctuidae) and Ephestia kuehniella (Pyralidae)

BenFarhat-Touzri

Driss

Tounsi

2016

Toxicon

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Section: Characterization Of Blb 250supporting

confidence: 64%

A promising HD133-like strain of Bacillus thuringiensis with dual efficiency to the two Lepidopteran pests: Spodoptera littoralis (Noctuidae) and Ephestia kuehniella (Pyralidae)

BenFarhat-Touzri

Driss

Tounsi

2016

Toxicon

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“…Some research (Bobrowski et al, 2001;Silva et al, 2004;Monnerat et al, 2007;Silva-Werneck and Ellar, 2008;Gobatto et al, 2010) has shown the possibility of finding B. thuringiensis native isolates with toxicity to A. gemmatalis. The bioassays screening results with new B. thuringiensis isolates are useful for local and regional insect pest management.…”

Section: Discussionmentioning

confidence: 99%

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Fiúza¹,

Schünemann

Pinto

et al. 2012

Braz. J. Biol.

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Bacillus thuringiensis is a bacterium used for biopesticides production and pest-resistant plants due to the synthesis of protein crystals by cry genes, which are effective in controlling several insect orders such as Lepidoptera. This work aimed at the evaluation and characterisation of two new B. thuringiensis isolates active against A. gemmatalis (Hübner 1818) larvae, which is the soybean major pest. The results showed that Bt117-4 isolate amplified fragments corresponding to cry2 and cry9 genes, and synthesised protein fragments equivalent to 130, 90 and 45 kDa. The Bt3146-4 isolate amplified DNA fragments corresponding to cry9 gene and synthesised protein fragments of 70, 58 and 38 kDa. Transmission electron microscopy revealed the presence of protein crystals in both isolates. CL50 with Cry purified proteins from Bt117-4 and Bt3146-4, corresponded to 0.195 and 0.191 µg larvae-1, respectively. The two B. thuringiensis isolates selected in this study were effective to control velvetbean caterpillar at laboratory conditions. Field tests should be carried on to develop new biopesticides formulation as well for cry genes resource for Anticarsia gemmatalis resistant transgenic plants.

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“…B. thuringiensis is a naturally occurring spore-forming, soil bacteria and diVerent preparations containing Bt are widely used as a microbial pesticide in agriculture. Bt has been eVectively used in control programs against lepidopteran larvae in Europe and North America (Bobrowski et al 2002). Although there are reports on the development of resistance against Bt, but the combination of Bt with the botanicals can be helpful to minimize the problem (Nathan et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Biological impact of harmaline, ricinine and their combined effects with Bacillus thuringiensis on Spodoptera exigua (Lepidoptera: Noctuidae)

Rizwan-ul-Haq

Mei

et al. 2009

J Pest Sci

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The history of botanical pesticides reveals that their study did mainly focus on the determination of acute median lethal dose or concentration. In the current situation, it is the dire need to understand the sublethal eVects of the botanical extracts along with the traditional studies of lethal concentrations in order to comprehensively investigate the future role of the botanical extracts as pesticides. This study reveals the eVects of traditionally used medicinal plant extracts harmaline (H) and ricinine (R) either individually or in combination with Bacillus thuringiensis (Bt) on the acute toxicity and sublethal eVects on the nutrition and enzyme system of Spodoptera exigua. Harmaline and ricinine caused reduction in the growth of neonate larvae up to 93.12 and 84.31%. The EC 50 values of harmaline against fourth and Wfth instars were 0.24 and 0.27 mg/ml, but these values remained 0.49 and 0.54 mg/ml against fourth and Wfth instars after being treated with ricinine. The combination of harmaline and ricinine with Bt resulted in the increased eYciency of these chemicals as the mortality percentages signiWcantly increased up to 96 and 87.82% in signiWcantly less exposure time in case of H + Bt and R + Bt respectively, as compared to individual treatments. The nutritional analysis revealed the increased toxicity of harmaline and ricinine in combination with Bt, but H + Bt2 showed the higher eYciency with minimal relative consumption rate 2.50 mg/mg/day, relative growth rate 1.16 mg/mg/day and eYciency of conversion of ingested food 29.66% of control, respectively. Changes in antioxidant enzymes such as superoxide dismutase (SOD) and catalases (CAT) were noticed to some extent over diVerent exposure times at all the treatments. The highest SOD (+37.29%) and CAT (+29.27%) activity was observed at the 6th day of treatment with H + R + Bt2. The study clearly shows the signiWcantly increased eYciency of harmaline and ricinine in combination with Bt against S. exigua. This phenomenon can be helpful in order to develop better control strategies against diVerent notorious pests.

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Characterization of two Bacillus thuringiensis isolates from South Brazil and their toxicity against Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Cited by 26 publications

References 27 publications

A promising HD133-like strain of Bacillus thuringiensis with dual efficiency to the two Lepidopteran pests: Spodoptera littoralis (Noctuidae) and Ephestia kuehniella (Pyralidae)

A promising HD133-like strain of Bacillus thuringiensis with dual efficiency to the two Lepidopteran pests: Spodoptera littoralis (Noctuidae) and Ephestia kuehniella (Pyralidae)

Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)

Biological impact of harmaline, ricinine and their combined effects with Bacillus thuringiensis on Spodoptera exigua (Lepidoptera: Noctuidae)

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