Characterization of two adenine nucleotide translocase paralogues in the stink bug, &lt;i&gt;Plautia stali&lt;/i&gt;

Sugahara, Ryohei; Minaba, Masaomi; Jouraku, Akiya; Kotaki, Toyomi; Yamamoto, Toshiharu; Shinohara, Yasuo; Miyoshi, Hideto; Shiotsuki, Takahiro

doi:10.1584/jpestics.d15-080

Cited by 7 publications

(13 citation statements)

References 18 publications

(14 reference statements)

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“…S2B), suggesting that a chronic shortage of ATP might have affected ecdysis, as discussed in a previous study (Sugahara et al, 2016a). The eclosion rates following silencing of PsPiC1 or PsPiC2 were higher than that obtained for PsANTI1 knockdown (0%; Sugahara et al, 2016a). Taken together, these results suggest that PsPiC paralogues are not similar to PsANT paralogues, which is consistent with the phylogenetic analyses: PsPiC paralogues formed a group (Fig.…”

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Musupporting

confidence: 87%

“…However, PsPiC1 and PsPiC2 were consistently expressed, even during ecdysis of third-instar nymphs (Fig. S2B), suggesting that a chronic shortage of ATP might have affected ecdysis, as discussed in a previous study (Sugahara et al, 2016a). The eclosion rates following silencing of PsPiC1 or PsPiC2 were higher than that obtained for PsANTI1 knockdown (0%; Sugahara et al, 2016a).…”

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 49%

“…We previously reported that Plau. stali harbours two ANT paralogues, PsANTI1 and PsANTI2 (Sugahara et al, 2016a). Although PsANTI1 was consistently expressed in all developmental stages and in all tissues, PsANTI2 transcripts were not detected in first-instar nymphs and adult antennae.…”

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 94%

“…Abbreviations: VE, dsVENUS; P-a, dsPiC1-a; P-U, dsPiC1-UTR; P-b, dsPiC1-b. (Sugahara et al, 2016a). To test if PsPiC1 and PsPiC2 have similar effects, their expression patterns were examined.…”

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 99%

“…The tissue-specific expression patterns of PiC and ANT isoforms imply that some components of these mitochondrial proteins vary according to tissue type. To date, several studies have identified insect ANT paralogues and examined their expression and silencing phenotypes (Zhang et al, 1999;Terhzaz et al, 2010;Sugahara et al, 2015aSugahara et al, , 2016a, but no such studies have been conducted for insect PiC. Determining the variations in mitochondrial protein components might help in understanding mitochondria-associated regulation in insects, such as ATP synthesis, apoptosis signalling and the membrane potential.…”

Section: Introductionmentioning

confidence: 99%

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Tissue‐specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species

Sugahara

Jouraku

Nakakura

et al. 2017

Insect Molecular Biology

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The mitochondrial phosphate carrier gene (PiC) encodes a membrane protein that mediates the supply of inorganic phosphate from the cytosol into the mitochondrial matrix. This substrate-specific transport system plays an important role in efficient ATP synthesis. Mammals appear to have only one PiC with two alternative splicing variants whose functional differences remain unclear. The present study is the first to characterize the multiple genes that encode PiC in insects. Bombyx mori was found to have two PiC paralogues, one ubiquitous and one testis-specific, the latter seeming to be present only in Lepidoptera. Drosophila melanogaster was found to harbour two PiC paralogues, whereas Liriomyza chinensis, another dipteran, has three PiC paralogues. Two PiCs were found to be present in Plautia stali, and silencing either of these genes affected the normal development of P. stali nymphs, although their expression patterns differed amongst tissues. Schistocerca gregaria and Locusta migratoria have two PiC each, with different expression patterns. Tribolium castaneum was found to have only one PiC, which appears to play an essential role in larval development. Thus, although the inorganic phosphate transport system appears to be conserved across eukaryotes, PiC has become specialized in the different tissues of different insect species.

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Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Musupporting

confidence: 87%

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 49%

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 94%

Section: Expression Levels Of Pspic1 and Pspic2 Differ Markedly In Mumentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tissue‐specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species

Sugahara

Jouraku

Nakakura

et al. 2017

Insect Molecular Biology

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E93 expression and links to the juvenile hormone in hemipteran mealybugs with insights on female neoteny

Vea

Tanaka

Tsuji

et al. 2019

Insect Biochemistry and Molecular Biology

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Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

et al. 2020

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Insect molting hormone (ecdysteroids) and juvenile hormone regulate molting and metamorphic events in a variety of insect species. Mealybugs undergo sexually dimorphic metamorphosis: males develop into winged adults through non-feeding, pupa-like stages called prepupa and pupa, while females emerge as neotenic wingless adults. We previously demonstrated, in the Japanese mealybug Planococcus kraunhiae (Kuwana), that the juvenile hormone titer is higher in males than in females at the end of the juvenile stage, which suggests that juvenile hormone may regulate male-specific adult morphogenesis. Here, we examined the involvement of ecdysteroids in sexually dimorphic metamorphosis. To estimate ecdysteroid titers, quantitative RT-PCR analyses of four Halloween genes encoding for cytochrome P450 monooxygenases in ecdysteroid biosynthesis, i.e., spook, disembodied, shadow and shade, were performed. Overall, their expression levels peaked before each nymphal molt. Transcript levels of spook, disembodied and shadow, genes that catalyze the steps in ecdysteroid biosynthesis in the prothoracic gland, were higher in males from the middle of the second nymphal instar to adult emergence. In contrast, the expression of shade, which was reported to be involved in the conversion of ecdysone into 20-hydroxyecdysone in peripheral tissues, was similar between males and females. These results suggest that ecdysteroid biosynthesis in the prothoracic gland is more active in males than in females, although the final conversion into 20-hydroxyecdysone occurs at similar levels in both sexes. Moreover, expression profiles of ecdysone response genes, ecdysone receptor and ecdysone-induced protein 75B, were also analyzed. Based on these expression profiles, we propose that the changes in ecdysteroid titer differ between males and females, and that high ecdysteroid titer is essential for directing male adult development.

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Characterization of two adenine nucleotide translocase paralogues in the stink bug, <i>Plautia stali</i>

Cited by 7 publications

References 18 publications

Tissue‐specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species

Tissue‐specific expression and silencing phenotypes of mitochondrial phosphate carrier paralogues in several insect species

E93 expression and links to the juvenile hormone in hemipteran mealybugs with insights on female neoteny

Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)

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