Characterization of Trichoderma spp. antagonistic to Phytophthora colocasiae associated with leaf blight of taro

Nath, Vishnu Sukumari; John, Neetha; Anjanadevi, Indira Parameswaran; Hegde, Vinayaka; Jeeva, M. L.; Misra, R. S.; Veena, S

doi:10.1007/s13213-013-0794-7

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…Numerous studies have been conducted on Trichoderma spp., with particular emphasis on mycoparasitic activities and their potential as biocontrol agents of plant diseases, including Rhizoctonia solani Kühn ( Almeida et al, 2007 ), Moniliophthora perniciosa (Stahel) Aime & Phillips-Mora ( De Souza et al, 2008 ), Pyricularia oryzae Cavara ( Dey et al, 2013 ), Phytophthora colocasiae Racib. ( Nath et al, 2014 ), Phytophthora megakarya Brasier & Griffin ( Mbarga et al, 2014 ), Phymatotrichopsis omnivora (Shear) Hennebert ( Guigón-López et al, 2015 ), Sclerotium cepivorum Berk. ( Rivera-Méndez et al, 2020 ), and Hemileia vastatrix Berk.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species

et al. 2023

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Fungi belonging to the genus Trichoderma have been widely recognized as efficient controllers of plant diseases. Although the majority of isolates currently deployed, thus far, have been isolated from soil, endophytic Trichoderma spp. is considered to be a promising option for application in biocontrol. In this study, 30 endophytic Trichoderma isolates—obtained from the leaves, stems, and roots of wild Hevea spp. in the Brazilian Amazon—were analyzed using specific DNA barcodes: sequences of internal transcribed spacers 1 and 2 of rDNA (ITS region), genes encoding translation elongation factor 1-α (TEF1-α), and the second largest subunit of RNA polymerase II (RPB2). The genealogical concordance phylogenetic species recognition (GCPSR) concept was used for species delimitation. A phylogenetic analysis showed the occurrence of Trichoderma species, such as T. erinaceum, T. ovalisporum, T. koningiopsis, T. sparsum, T. lentiforme, T. virens, and T. spirale. Molecular and morphological features resulted in the discovery of four new species, such as T. acreanum sp. nov., T. ararianum sp. nov., T. heveae sp. nov., and T. brasiliensis sp. nov. The BI and ML analyses shared a similar topology, providing high support to the final trees. The phylograms show three distinct subclades, namely, T. acreanum and T. ararianum being paraphyletic with T. koningiopsis; T. heveae with T. subviride; and T. brasiliensis with T. brevicompactum. This study adds to our knowledge of the diversity of endophytic Trichoderma species in Neotropical forests and reveals new potential biocontrol agents for the management of plant diseases.

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Section: Introductionmentioning

confidence: 99%

Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species

et al. 2023

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“…Trichoderma spp. have been reported to exhibit antagonistic activity against a broad spectrum of plant pathogenic fungi and oomycetes, such as Fusarium oxysporum [ 6 , 7 ], Phytophthora colocasiae [ 8 ], and Pythium graminicola [ 9 ], as well as plant pathogenic bacteria [ 10 ] and nematodes [ 11 ]. The many secondary metabolites produced by Trichoderma species are responsible for their highly antagonistic effects, contributing to the success of their application [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings

Rao¹,

Zeng²,

Jiang³

et al. 2022

BMC Microbiol

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Background The promotion of plant growth and suppression of plant disease using beneficial microorganisms is considered an alternative to the application of chemical fertilizers or pesticides in the field. Results A coconut-scented antagonistic Trichoderma strain LZ42, previously isolated from Ganoderma lucidum-cultivated soil, was investigated for biostimulatory and biocontrol functions in tomato seedlings. Morphological and phylogenetic analyses suggested that strain LZ42 is closely related to T. atroviride. Tomato seedlings showed increased aerial and root dry weights in greenhouse trials after treatment with T. atroviride LZ42 formulated in talc, indicating the biostimulatory function of this fungus. T. atroviride LZ42 effectively suppressed Fusarium wilt disease in tomato seedlings, with an 82.69% control efficiency, which is similar to that of the carbendazim treatment. The volatile organic compounds (VOCs) emitted by T. atroviride LZ42 were found to affect the primary root growth direction and promote the root growth of tomato seedlings in root Y-tube olfactometer assays. The fungal VOCs from T. atroviride LZ42 were observed to significantly inhibit F. oxysporum in a sandwiched Petri dish assay. SPME–GC–MS analysis revealed several VOCs emitted by T. atroviride LZ42; the dominant compound was tentatively identified as 6-pentyl-2H-pyran-2-one (6-PP). The VOC 6-PP exhibited a stronger ability to influence the direction of the primary roots of tomato seedlings but not the length of the primary roots. The inhibitory effect of 6-PP on F. oxysporum was the highest among the tested pure VOCs, showing a 50% effective concentration (EC50) of 5.76 μL mL−1 headspace. Conclusions Trichoderma atroviride LZ42, which emits VOCs with multiple functions, is a promising agent for the biostimulation of vegetable plants and integrated management of Fusarium wilt disease.

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“…Trichoderma spp. have been reported to exhibit antagonistic activity against a broad spectrum of plant pathogenic fungi and oomycetes, such as Fusarium oxysporum [6,7] and Phytophthora colocasiae [8], as well as plant pathogenic bacteria [9] and nematodes [10]. The many secondary metabolites produced by Trichoderma species are responsible for their highly antagonistic effects, contributing to the success of their application [11].…”

Section: Introductionmentioning

confidence: 99%

Trichoderma atroviride LZ42 Releases Volatile Organic Compounds Promoting Plant Growth and Resisting Fusarium wilt Disease in Tomatoes

Jiang¹,

Rao²,

Zeng³

et al. 2021

Preprint

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The promotion of plant growth and suppression of plant disease using beneficial microorganisms is considered an alternative to the application of chemical fertilizers or pesticides in the field. In this study, a coconut-scented antagonistic Trichoderma strain LZ42, previously isolated from Genoderma lucidum-cultivated soil, was investigated for biostimulatory and biocontrol functions in tomato seedlings. Morphological and phylogenetic analyses suggested that strain LZ42 is closely related to T. atroviride. Tomato plants showed increased aerial and root dry weights in greenhouse trials after treatment with T. atroviride LZ42 formulated in talc, indicating the biostimulatory function of this fungus. T. atroviride LZ42 effectively suppressed Fusarium wilt disease in tomato seedlings, with an 82.69% control efficiency, which is similar to that of fungicide treatment. The volatile organic compounds (VOCs) emitted by T. atroviride LZ42 were found to affect the primary root growth direction and promote the root growth of tomato seedlings in root Y-tube olfactometer assays. The fungal VOCs from T. atroviride LZ42 were observed to significantly inhibit F. oxysporum in a sandwiched Petri dish assay. SPME-GC-MS analysis revealed several VOCs emitted by T. atroviride LZ42; the dominant compound was tentatively identified as 6-pentyl-2H-pyran-2-one (6-PP). 6-PP exhibited a stronger ability to influence the direction of the primary roots of tomato seedlings but not the length of the primary roots. The inhibitory effect of 6-PP on F. oxysporum was the highest among the tested pure VOCs, showing a 50% effective concentration (EC50) of 5.76 μL mL-1 headspace. In conclusion, T. atroviride LZ42, which emits VOCs with multiple functions, is a promising agent for the biostimulation of vegetable plants and integrated management of Fusarium wilt disease.

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Characterization of Trichoderma spp. antagonistic to Phytophthora colocasiae associated with leaf blight of taro

Cited by 6 publications

References 41 publications

Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species

Endophytic Trichoderma species from rubber trees native to the Brazilian Amazon, including four new species

Trichoderma atroviride LZ42 releases volatile organic compounds promoting plant growth and suppressing Fusarium wilt disease in tomato seedlings

Trichoderma atroviride LZ42 Releases Volatile Organic Compounds Promoting Plant Growth and Resisting Fusarium wilt Disease in Tomatoes

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