Characterization of trehalose metabolic genes and corresponding enzymatic activities during diapause of Sitodiplosis mosellana

Huang, Qitong; Zhang, Guojun; Nan, Jianglei; Cheng, Weining; Zhu‐Salzman, Keyan

doi:10.1016/j.jinsphys.2021.104324

Cited by 17 publications

(10 citation statements)

References 53 publications

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“…The accumulation of cryoprotective solutes (polyols, sugars, and free amino acids) might contribute to the acquisition of cold tolerance in many insects, thereby improving the individual survival rates. Recent studies have reported that trehalose has high a concentration in the insect hemolymph, and it is the crucial ‘blood sugar’ that protects the cells against harsh environmental conditions [ 29 , 30 , 31 ]. Several studies have demonstrated the protective function of trehalose against potential harm that is induced by cold stress in overwintering insects.…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Ice-Nucleation-Active Bacteria on Metabolic Regulation in Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) Overwintering Larvae on the Qinghai-Tibet Plateau

Shao

Liu

et al. 2022

Insects

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Evergestis extimalis (Scopoli) is a significant pest of spring oilseed rape in the Qinghai-Tibet Plateau. It has developed resistance to many commonly used insecticides. Therefore, biopesticides should be used to replace the chemical pesticides in pest control. In this study, the effects of ice-nucleation-active (INA) microbes (Pseudomonas syringae 1.7277, P. syringae 1.3200, and Erwinia pyrifoliae 1.3333) on E. extimalis were evaluated. The supercooling points (SCP) were markedly increased due to the INA bacteria application when they were compared to those of the untreated samples. Specifically, the SCP of E. extimalis after its exposure to a high concentration of INA bacteria in February were -10.72℃, -13.73℃, and -14.04℃. Our findings have demonstrated that the trehalase (Tre) genes were up-regulated by the application of the INA bacteria, thereby resulting in an increased trehalase activity. Overall, the INA bacteria could act as effective heterogeneous ice nuclei which could lower the hardiness of E. extimalis to the cold and then freeze them to death in an extremely cold winter. Therefore, the control of insect pests with INA bacteria goes without doubt, in theory.

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Section: Discussionmentioning

confidence: 99%

“…Trehalose, a non-reducing disaccharide that consists of two glucose molecules, is present in several organisms, including insects, plants, bacteria, fungi, and other invertebrates [ 29 , 35 ]. The only known pathway of cellular trehalose utilization is its hydrolysis by trehalase in insects [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Ice-Nucleation-Active Bacteria on Metabolic Regulation in Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) Overwintering Larvae on the Qinghai-Tibet Plateau

Shao

Liu

et al. 2022

Insects

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“…[6,[9][10] TPP has been studied in a few insects like Delia antiqua, Anopheles gambie, Bactrocera minax, and Sitodiplosis mosellana. [9,6,[11][12] Three TPP genes from Bactrocera minax express highly in the last instar larvae or early pupal stages. [6] The expression and activity of TPP are elevated in the pre-diapause and diapause stages of Sitodiplosis mosellana.…”

Section: Introductionmentioning

confidence: 99%

“…[ 6 ] The expression and activity of TPP are elevated in the pre‐diapause and diapause stages of Sitodiplosis mosellana . [ 12 ] Thus, inhibition of TPP presents an excellent model to elucidate the effect of trehalose on insect development and physiology.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Trehalose Synthesis in Lepidoptera Reduces Larval Fitness

Tellis,

Mohite,

Nair

et al. 2023

Advanced Biology

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Trehalose is synthesized in insects through the trehalose 6‐phosphate synthase and phosphatase (TPS/TPP) pathway. TPP dephosphorylates trehalose 6‐phosphate to release trehalose. Trehalose is involved in metamorphosis, but its relation with body weight, size, and developmental timing is unexplored. The expression and activity of TPS/TPP fluctuate depending on trehalose demand. Thus, TPS/TPP inhibition can highlight the significance of trehalose in insect physiology. TPS/TPP transcript levels are elevated in the pre‐pupal and pupal stages in Helicoverpa armigera. The inhibition of recombinantly expressed TPP by N‐(phenylthio)phthalimide (NPP), is validated by in vitro assays. In vivo inhibition of trehalose synthesis reduces larval weight and size, hampers metamorphosis, and reduces its overall fitness. Insufficient trehalose leads to a shift in glucose flux, reduced energy, and dysregulated fatty acid oxidation. Metabolomics reaffirms the depletion of trehalose, glucose, glucose 6‐phosphate, and suppressed tricarboxylic acid cycle. Reduced trehalose hampers the energy level affecting larval vitality. Through trehalose synthesis inhibition, the importance of trehalose in insect physiology and development is investigated. Also, in two other lepidopterans, TPP inhibition impedes physiology and survival. NPP is also found to be effective as an insecticidal formulation. Overall, trehalose levels affect the larval size, weight, and metabolic homeostasis for larval‐pupal transition in lepidoptera.

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“…Tre (EC 3.2.1.28) is a glycosidase that is commonly found in bacteria, fungi, plants, and animals [ 15 , 16 ]. It catalyzes trehalose to produce two molecules of glucose, which are then used for various physiological and life activities [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed Planthopper Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

et al. 2022

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Trehalase (Tre) is a crucial enzyme involved in trehalose metabolism, and it plays pivotal roles in insect development and metamorphosis. However, the biological function of Tre genes in Sogatella furcifera remains unclear. In the present study, two Tre genes—SfTre1 and SfTre2—were cloned and identified based on the S. furcifera transcriptome data. Bioinformatic analysis revealed that the full-length complementary DNA of SfTre1 and SfTre2 genes were 3700 and 2757 bp long, with 1728- and 1902-bp open reading frame encoding 575 and 633 amino acid residues, respectively. Expression analysis indicated that SfTre1 and SfTre2 were expressed at all developmental stages, with the highest expression in day two adults. Furthermore, the highest expression levels of SfTre1 and SfTre2 were observed in the ovary; enriched expression was also noted in head tissues. The knockdown of SfTre1 and SfTre2 via injecting double-stranded RNAs decreased the transcription levels of the corresponding mRNAs and led to various malformed phenotypes and high lethality rates. The results of our present study indicate that SfTre1 and SfTre2 play crucial roles in S. furcifera growth and development, which can provide referable information for Tre genes as a potential target for planthopper control.

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Characterization of trehalose metabolic genes and corresponding enzymatic activities during diapause of Sitodiplosis mosellana

Cited by 17 publications

References 53 publications

The Effect of Ice-Nucleation-Active Bacteria on Metabolic Regulation in Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) Overwintering Larvae on the Qinghai-Tibet Plateau

The Effect of Ice-Nucleation-Active Bacteria on Metabolic Regulation in Evergestis extimalis (Scopoli) (Lepidoptera: Pyralidae) Overwintering Larvae on the Qinghai-Tibet Plateau

Inhibition of Trehalose Synthesis in Lepidoptera Reduces Larval Fitness

Knockdown of Two Trehalase Genes by RNA Interference Is Lethal to the White-Backed Planthopper Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

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