Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis

Zhang, Ziyang; Cheng, Ling; Zhang, Weihua; Hu, Jianlin; Liu, Yan; Lin, Yongwen

doi:10.1007/s00468-020-01994-9

Cited by 6 publications

(7 citation statements)

References 25 publications

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“…In order to determine whether PbrbZIP15 exhibited a transcription activation capability in yeast, its CDS was amplified and subsequently inserted into the pGBKT7 vector (BD) to generate the BD‐PbrbZIP15 recombinant plasmid (Zhang et al., 2020). This recombinant vector was then introduced into the Y2H Gold strain (Weidi, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

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PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

Jia,

Zhang,

Zhang

et al. 2023

The Plant Journal

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SUMMARYThe composition and abundance of soluble sugars in mature pear (Pyrus) fruit are important for its acceptance by consumers. However, our understanding of the genes responsible for soluble sugar accumulation remains limited. In this study, a S1‐group member of bZIP gene family, PbrbZIP15, was characterized from pear genome through the combined analyses of metabolite and transcriptome data followed by experimental validation. PbrbZIP15, located in nucleus, was found to function in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli. After analyzing the expression profiles of sugar‐metabolism‐related genes and the distribution of cis‐acting elements in their promoters, the glucose isomerase 1 gene (PbrXylA1), whose corresponding protein catalyzed the isomerization of glucose and fructose in vitro, was identified as a downstream target gene of PbrbZIP15. PbrbZIP15 could directly bind to the G‐box element in PbrXylA1 promoter and activate its transcription, as evidenced by chromatin immunoprecipitation‐quantitative PCR, yeast one‐hybrid, electrophoretic mobility shift assay, and dual‐luciferase assay. PbrXylA1, featuring a leucine‐rich signal peptide in its N‐terminal, was localized to the endoplasmic reticulum. It was validated to play a significant role in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli, which was associated with the upregulated fructose/glucose ratio. Further studies revealed a positive correlation between the sucrose content and the expression levels of several sucrose‐biosynthesis‐related genes (PbrFRK3/8, PbrSPS1/3/4/8, and PbrSPP1) in PbrbZIP15‐/PbrXylA1‐transgenic fruit/calli. In conclusion, our results suggest that PbrbZIP15‐induced soluble sugar accumulation during pear development is at least partly attributed to the activation of PbrXylA1 transcription.

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Section: Methodsmentioning

confidence: 99%

“…This recombinant vector was then introduced into the Y2H Gold strain (Weidi, Shanghai, China). After transformation for 3 days, the yeast harboring BD‐PbrbZIP15 was dripped on to SD/−Trp medium with or without AbA (Zhang et al., 2020). The yeast transformed with the empty BD was employed as the negative control.…”

Section: Methodsmentioning

confidence: 99%

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

Jia,

Zhang,

Zhang

et al. 2023

The Plant Journal

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“…Co-localization of SIZ1 with substrates was also shown to occur in the nucleus. Indeed, bimolecular fluorescence complementation assays indicate that the interaction between eucalyptus SIZ1 and ICE1 (Zhang et al, 2020b) and between Arabidopsis SIZ1 and COP1 (Lin et al, 2016) both occur in the nucleus. The exact sub-cellular localization of PIALs is unknown, although their physical and functional interaction with the nuclear protein MOM1 is consistent with a nuclear localization (Han et al, 2016;Zhao and He, 2018).…”

Section: Localization and Connection With Dnamentioning

confidence: 99%

Plant SUMO E3 Ligases: Function, Structural Organization, and Connection With DNA

Jmii

Cappadocia

2021

Front. Plant Sci.

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Protein modification by the small ubiquitin-like modifier (SUMO) plays an important role in multiple plant processes, including growth, development, and the response to abiotic stresses. Mechanistically, SUMOylation is a sequential multi-enzymatic process where SUMO E3 ligases accelerate SUMO conjugation while also influencing target identity and interactions. This review explores the biological functions of plant SUMO E3 ligases [SAP AND MIZ1 DOMAIN-CONTAINING LIGASE (SIZs), METHYL METHANESULFONATE-SENSITIVITY PROTEIN 21 (MMS21s), and PROTEIN INHIBITOR OF ACTIVATED STAT-LIKE (PIALs)] in relation to their molecular activities and domains. We also explore the sub-cellular localization of SUMO E3 ligases and review evidence suggesting a connection between certain SUMO E3 ligases and DNA that contributes to gene expression regulation.

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“…Our previous study revealed that the overexpression of the EcaICE1 gene of E. camaldulensis improved the cold tolerance of transgenic tobacco plants (Lin et al 2014). Recently, we demonstrated that the EcaSIZ1 (Zhang et al 2020) and EcaHOS1 (Cheng et al 2021) proteins interact with the EcaICE1 protein in the nucleus, and that Ser 158 of EcaICE1 was the probable key putative site that mediated its interaction with EcaHOS1 (Cheng et al 2021). In this study, we used a high-throughput sequencing approach for predicting and identifying the miR-NAs that were likely to target the ICE-CBF-COR pathway.…”

Section: Introductionmentioning

confidence: 98%

“…Recently, we demonstrated that the EcaSIZ1 (Zhang et al . 2020) and EcaHOS1 (Cheng et al . 2021) proteins interact with the EcaICE1 protein in the nucleus, and that Ser 158 of EcaICE1 was the probable key putative site that mediated its interaction with EcaHOS1 (Cheng et al .…”

Section: Introductionmentioning

confidence: 99%

High‐throughput miRNA sequencing and identification of a novel ICE1‐targeting miRNA in response to low temperature stress in Eucalyptus camaldulensis

Liu

et al. 2023

Plant Biol J

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MicroRNAs (miRNAs) play a crucial role in the growth, development, morphogenesis, signal transduction, and stress response in plants. The ICE (Inducer of CBF expression)‐CBF (C‐repeat binding factor)‐COR (Cold‐regulated gene) regulatory cascade is an important signalling pathway in plant response to low temperature stress, and it remains unknown whether this pathway is regulated by miRNAs. In this study, high‐throughput sequencing was employed for predicting and identifying the miRNAs that were likely to target the ICE‐CBF‐COR pathway in Eucalyptus camaldulensis. A novel ICE1‐targeting miRNA, eca‐novel‐miR‐259‐5p (nov‐miR259), was further analysed. A total of 392 conserved miRNAs and 97 novel miRNAs were predicted, including 80 differentially expressed miRNAs. Of these, 30 miRNAs were predicted to be associated with the ICE‐CBF‐COR pathway. The full‐length of mature nov‐miR259 was 22 bp and its precursor gene was 60 bp in length, with a typical hairpin structure. The RNA ligase‐mediated 5′ amplification of cDNA ends (5′‐RLM‐RACE) and Agrobacterium‐mediated tobacco transient expression assays demonstrated that nov‐miR259 could cleave EcaICE1 in vivo. Moreover, qRT‐PCR and Pearson's correlation analysis further revealed that the expression levels of nov‐miR259 were almost significantly negatively correlated with those of its target gene, EcaICE1, and the other genes in the ICE‐CBF‐COR pathway. We first identified the nov‐miR259 as a novel ICE1‐targeting miRNA, and the nov‐miR259‐ICE1 module may be involved in regulating the cold stress response in E. camaldulensis.

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Characterization of transcription activation domain of EcaICE1 and its interaction with EcaSIZ1 in Eucalyptus camaldulensis

Cited by 6 publications

References 25 publications

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

PbrbZIP15 promotes sugar accumulation in pear via activating the transcription of the glucose isomerase gene PbrXylA1

Plant SUMO E3 Ligases: Function, Structural Organization, and Connection With DNA

High‐throughput miRNA sequencing and identification of a novel ICE1‐targeting miRNA in response to low temperature stress in Eucalyptus camaldulensis

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