Characterization of three novel β-galactosidases from Akkermansia muciniphila involved in mucin degradation

Kosciow, Konrad; Deppenmeier, Uwe

doi:10.1016/j.ijbiomac.2020.01.246

Cited by 39 publications

(27 citation statements)

References 49 publications

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“…However, the rate of hydrolysis of the reaction was slow and we were unable to determine the kinetics of this enzyme on lactose. This finding is consistent with that of Kosciow et al who observed that Amuc_0771 exhibited low relative hydrolytic activity against β1-4 linked galactose to glucose (lactose) and β1-4 linked galactose to N-acetyl-d-glucosamine (LacNAc) 51 . Guo et al, recently characterised the enzymatic activity of an A. muciniphila GH35 β-galactosidase, Am0874.…”

Section: Discussionsupporting

confidence: 92%

Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro

Kostopoulos

Elzinga

Ottman

et al. 2020

Sci Rep

110

106

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Akkermansia muciniphila is a well-studied anaerobic bacterium specialized in mucus degradation and associated with human health. Because of the structural resemblance of mucus glycans and free human milk oligosaccharides (HMos), we studied the ability of A. muciniphila to utilize human milk oligosaccharides. We found that A. muciniphila was able to grow on human milk and degrade HMos. Analyses of the proteome of A. muciniphila indicated that key-glycan degrading enzymes were expressed when the bacterium was grown on human milk. our results display the functionality of the key-glycan degrading enzymes (α-l-fucosidases, β-galactosidases, exo-α-sialidases and β-acetylhexosaminidases) to degrade the HMO-structures 2′-FL, LNT, lactose, and LNT2. The hydrolysation of the host-derived glycan structures allows A. muciniphila to promote syntrophy with other beneficial bacteria, contributing in that way to a microbial ecological network in the gut. Thus, the capacity of A. muciniphila to utilize human milk will enable its survival in the early life intestine and colonization of the mucosal layer in early life, warranting later life mucosal and metabolic health.

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Section: Discussionsupporting

confidence: 92%

Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro

Kostopoulos

Elzinga

Ottman

et al. 2020

Sci Rep

110

106

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“…An inflamed area in the gut will have a reduced mucus layer (van der Post et al, 2019) and elevated host defense responses (Zhang et al, 2018a). The host mucus layer is a nutritional source of cross-feeding in the gut microbiome (Bunesova et al, 2018;Kosciow and Deppenmeier, 2020;Schroeder, 2019). Loss of this layer may firstly affect the network hub functions of carbohydrate and amino acid metabolism, and subsequently affect the functional interactions in the whole community.…”

Section: Discussionmentioning

confidence: 99%

Revealing Protein-Level Functional Redundancy in the Human Gut Microbiome using Ultra-deep Metaproteomics

Ning

Zhang

et al. 2021

Preprint

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Functional redundancy is a key property of ecosystems and represents the fact that phylogenetically unrelated taxa can play similar functional roles within an ecosystem. The redundancy of potential functions of human microbiome has been recently quantified using metagenomics data. Yet, the redundancy of functions which are actually expressed within the human microbiome remains largely unexplored. Here, we quantify the protein-level functional redundancy in the human gut microbiome using metaproteomics and network approaches. In particular, our ultra-deep metaproteomics approach revealed high protein-level functional redundancy and high nestedness in proteomic content networks - bipartite graphs that connect taxa with their expressed functions. We further examined multiple metaproteomics datasets and showed that various environmental factors, including individuality, biogeography, xenobiotics, and disease, significantly altered the protein-level functional redundancy. Finally, by projecting the bipartite proteomic content networks into unipartite weighted genus networks, functional hub genera across individual microbiomes were discovered, suggesting that there may be a universal principle of functional organization in microbiome assembly.

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“…In addition, next generation probiotic candidates, such as Akkermansia muciniphila, some Bacillus spp. and Propionibacterium freudenreichii-which belong to GRAS (Generally Recognized As Safe) microorganisms-and yeasts of the genus Saccharomyces exhibit probiotic characteristics [39][40][41]. For a strain to be considered probiotic, it needs to be resistant to host-induced stressors, where it should show an ability to adhere and/or proliferate at the site of action.…”

Section: Modulation By Probioticsmentioning

confidence: 99%

Co-Encapsulated Synbiotics and Immobilized Probiotics in Human Health and Gut Microbiota Modulation

Kvaková

Bertková

Štofilová

et al. 2021

Foods

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Growing interest in the development of innovative functional products as ideal carriers for synbiotics, e.g., nutrient bars, yogurt, chocolate, juice, ice cream, and cheese, to ensure the daily intake of probiotics and prebiotics, which are needed to maintain a healthy gut microbiota and overall well-being, is undeniable and inevitable. This review focuses on the modern approaches that are currently being developed to modulate the gut microbiota, with an emphasis on the health benefits mediated by co-encapsulated synbiotics and immobilized probiotics. The impact of processing, storage, and simulated gastrointestinal conditions on the viability and bioactivity of probiotics together with prebiotics such as omega-3 polyunsaturated fatty acids, phytochemicals, and dietary fibers using various delivery systems are considered. Despite the proven biological properties of synbiotics, research in this area needs to be focused on the proper selection of probiotic strains, their prebiotic counterparts, and delivery systems to avoid suppression of their synergistic or complementary effect on human health. Future directions should lead to the development of functional food products containing stable synbiotics tailored for different age groups or specifically designed to fulfill the needs of adjuvant therapy.

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Characterization of three novel β-galactosidases from Akkermansia muciniphila involved in mucin degradation

Cited by 39 publications

References 49 publications

Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro

Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro

Revealing Protein-Level Functional Redundancy in the Human Gut Microbiome using Ultra-deep Metaproteomics

Co-Encapsulated Synbiotics and Immobilized Probiotics in Human Health and Gut Microbiota Modulation

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