Characterization of three male-sterile mutants of Arabidopsis thaliana exhibiting alterations in meiosis

Peirson, Brenda N.; Owen, Heather A.; Feldmann, Kenneth A.; Makaroff, Christopher A.

doi:10.1007/bf00230361

Cited by 64 publications

(64 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TUNEL assay detects in situ DNA cleavage, a hallmark feature of apoptosis-like PCD, by enzymatically incorporating fluorescein-12-dUTP into the 39-OH ends of fragmented DNA. Anther staging was based primarily on floral bud size and stages; however, key anther morphology traits that are unaffected in the myb80 mutant, such as tetrad formation corresponding to anther stage 7 and tetrad release at stage 8, were also used as an indicator of anther developmental stages (Smyth et al, 1990;Bowman et al, 1991;Peirson et al, 1996;Sanders et al, 1999). In the myb80 mutant, tapetal and microspore degeneration commences at stage 9, resulting in distorted morphology.…”

Section: Apoptosis-like Pcd Occurs Prematurely In Myb80 and Undead Tamentioning

confidence: 99%

“…Flower bud length was measured and staged according to Peirson et al (1996). The anthers at stages 5 to 8 (0.4 to 0.9 mm) were dissected in RNAlater solution (Ambion).…”

Section: Rt-pcr and Qrt-pcr Analysismentioning

confidence: 99%

“…GUS staining was examined under a dissecting microscope. Determination of anther stage was based on bud measurements and stages of anther development described by Peirson et al (1996) and reconciled with anther stages described by Sanders et al (1999). Anthers were stained with Alexander's stain (Alexander, 1969) and examined microscopically.…”

Section: Histochemical Assay Of Transformed Arabidopsis Plantsmentioning

confidence: 99%

See 2 more Smart Citations

The MYB80 Transcription Factor Is Required for Pollen Development and the Regulation of Tapetal Programmed Cell Death inArabidopsis thaliana

et al. 2011

View full text Add to dashboard Cite

Arabidopsis thaliana MYB80 (formerly MYB103) is expressed in the tapetum and microspores between anther developmental stages 6 and 10. MYB80 encodes a MYB transcription factor that is essential for tapetal and pollen development. Using microarray analysis of anther mRNA, we identified 404 genes differentially expressed in the myb80 mutant. Employing the glucocorticoid receptor system, the expression of 79 genes was changed when MYB80 function was restored in the myb80 mutant following induction by dexamethasone. Thirty-two genes were analyzed using chromatin immunoprecipitation, and three were identified as direct targets of MYB80. The genes encode a glyoxal oxidase (GLOX1), a pectin methylesterase (VANGUARD1), and an A1 aspartic protease (UNDEAD). All three genes are expressed in the tapetum and microspores. Electrophoretic mobility shift assays confirmed that MYB80 binds to all three target promoters, with the preferential binding site containing the CCAACC motif. TUNEL assays showed that when UNDEAD expression was silenced using small interfering RNA, premature tapetal and pollen programmed cell death occurred, resembling the myb80 mutant phenotype. UNDEAD possesses a mitochondrial targeting signal and may hydrolyze an apoptosis-inducing protein(s) in mitochondria. The timing of tapetal programmed cell death is critical for pollen development, and the MYB80/UNDEAD system may regulate that timing.

show abstract

Section: Apoptosis-like Pcd Occurs Prematurely In Myb80 and Undead Tamentioning

confidence: 99%

“…Flower bud length was measured and staged according to Peirson et al (1996). The anthers at stages 5 to 8 (0.4 to 0.9 mm) were dissected in RNAlater solution (Ambion).…”

Section: Rt-pcr and Qrt-pcr Analysismentioning

confidence: 99%

Section: Histochemical Assay Of Transformed Arabidopsis Plantsmentioning

confidence: 99%

See 1 more Smart Citation

The MYB80 Transcription Factor Is Required for Pollen Development and the Regulation of Tapetal Programmed Cell Death inArabidopsis thaliana

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Some of the mutants have been extensively characterized in major crop and model species, including wheat [109], maize [see 110 for a review, 111], rice [112][113][114][115], tomato (L. esculentum) [116], soybean [117], and Arabidopsis thaliana [118][119][120][121][122]. Asynapsis and desynapsis are usually conditioned by single recessive genes, such as st2, st3, and st8 for asynapsis and st4 and st5 for desynapsis in soybean [117,123,124], dy and dsy1 for desynapsis and phs1 for poor homologous synapsis in maize [111,125], and spo11-1-3, dsy1, mpa1, and asy1 in Arabidopsis thaliana [118][119][120][121][122]. In addition to single genes, duplicated recessive factors (st6 and st7) for desynapsis were identified in soybean [126].…”

Section: Meiotic Chromosome Segregation and Aneuploidymentioning

confidence: 99%

Meiosis-Driven Genome Variation in Plants

Cai¹,

Xu²

2007

View full text Add to dashboard Cite

Meiosis includes two successive divisions of the nucleus with one round of DNA replication and leads to the formation of gametes with half of the chromosomes of the mother cell during sexual reproduction. It provides a cytological basis for gametogenesis and inheritance in eukaryotes. Meiotic cell division is a complex and dynamic process that involves a number of molecular and cellular events, such as DNA and chromosome replication, chromosome pairing, synapsis and recombination, chromosome segregation, and cytokinesis. Meiosis maintains genome stability and integrity over sexual life cycles. On the other hand, meiosis generates genome variations in several ways. Variant meiotic recombination resulting from specific genome structures induces deletions, duplications, and other rearrangements within the genic and non-genic genomic regions and has been considered a major driving force for gene and genome evolution in nature. Meiotic abnormalities in chromosome segregation lead to chromosomally imbalanced gametes and aneuploidy. Meiotic restitution due to failure of the first or second meiotic division gives rise to unreduced gametes, which triggers polyploidization and genome expansion. This paper reviews research regarding meiosis-driven genome variation, including deletion and duplication of genomic regions, aneuploidy, and polyploidization, and discusses the effect of related meiotic events on genome variation and evolution in plants. Knowledge of various meiosis-driven genome variations provides insight into genome evolution and genetic variability in plants and facilitates plant genome research.

show abstract

“…In addition, meiotic mutants have been identified in numerous plants species, including Arabidopsis (Dawson et al, 1993;Chaudhury et al, 1994;He et al, 1996;Peirson et al, 1996;Ross et al, 1997;Spielman et al, 1997;Glover et al, 1998), maize (Golubovskaya et al, 1993(Golubovskaya et al, , 1997Maguire et al, 1993;Staiger and Cande, 1993), rice Omura, 1983, 1984), and tomato (Moens, 1969). The characterization of meiotic mutants and their use in the isolation of the corresponding genes are beginning to provide further insight into the molecular events that accompany meiosis in plants.As part of studies to better understand meiosis in plants, we have characterized a number of T-DNA-tagged malesterile mutants of Arabidopsis (Peirson et al, 1996). Cytological studies with one mutant line, syn1 , indicated that it produced polyads containing up to eight microspores with variable amounts of DNA.…”

mentioning

confidence: 99%

Isolation and Characterization of SYN1, a RAD21-like Gene Essential for Meiosis in Arabidopsis

et al. 1999

View full text Add to dashboard Cite

The proper pairing, recombination, and segregation of chromosomes are central to meiosis and sexual reproduction. The syn1 mutation was previously identified as a synaptic mutant in a T-DNA-tagged population of plants. SYN1 has been isolated and found to exhibit similarity to Schizosaccharomyces pombe RAD21 and RAD21-like proteins, which are required for chromosome condensation and sister chromatid cohesion during mitosis. Plants homozygous for syn1 are male and female sterile and show defects in chromosome condensation and pairing beginning at leptonema of meiosis I. Fragmentation of the chromosomes was observed at metaphase I. Alternative promoters produced two SYN1 transcripts. One transcript was expressed at low levels in most tissues, whereas the other was expressed only in prebolting buds. DNA blot analyses suggest that Arabidopsis contains a small RAD21 gene family. Consistent with the DNA blot data, a second Arabidopsis RAD21 -like gene has been identified. These results suggest that different RAD21-like proteins play essential roles in chromosome condensation and pairing during both meiosis and mitosis. INTRODUCTIONThe normal segregation of chromosomes during meiosis in most eukaryotic organisms is dependent on the successful pairing of homologous chromosomes during the zygotene stage of prophase I. After DNA replication, each chromosome enters meiosis as two chromatids. During leptonema of meiotic prophase I, the dispersed chromosomes condense to form long thin threads. During zygonema, homologous chromosomes align, and the synaptonemal complex, an elaborate proteinaceous structure that holds homologs closely apposed along their lengths, is formed. At pachynema, nonsister chromatids of the paired chromosomes recombine, forming chiasmata, which become visible as the chromosomes start to desynapse during diplonema. During meiosis I, sister chromosomes must be attached so that they can orient properly and achieve reductive division. During anaphase I, sister chromosome cohesion is released in preparation for the second meiotic division. Therefore, the formation and proper maintenance of sister chromatid cohesion are essential for the proper alignment and segregation of chromosomes during meiosis (Miyazaki and Orr-Weaver, 1994).A considerable amount of information is available on the events associated with meiosis in a number of eukaryotic systems, including plants (Dawe, 1998). However, much less is known about the genes required during meiosis in plants than in several other systems, including yeast (Roeder, 1995), Drosophila (Orr-Weaver, 1995), and Caenorhabditis elegans (Zetka and Rose, 1995). Based on similarities to meiotic genes identified in other organisms, several genes expressed during meiosis have been identified in Arabidopsis (Sato et al., 1995;Klimyuk and Jones, 1997), lily (Kobayashi et al., 1994), and wheat (Ji and Langridge, 1994). In addition, meiotic mutants have been identified in numerous plants species, including Arabidopsis (Dawson et al., 1993;Chaudhury et al., 1994;He et al., 1996...

show abstract

Characterization of three male-sterile mutants of Arabidopsis thaliana exhibiting alterations in meiosis

Cited by 64 publications

References 42 publications

The MYB80 Transcription Factor Is Required for Pollen Development and the Regulation of Tapetal Programmed Cell Death inArabidopsis thaliana

The MYB80 Transcription Factor Is Required for Pollen Development and the Regulation of Tapetal Programmed Cell Death inArabidopsis thaliana

Meiosis-Driven Genome Variation in Plants

Isolation and Characterization of SYN1, a RAD21-like Gene Essential for Meiosis in Arabidopsis

Contact Info

Product

Resources

About