Characterization of thermal, mechanical and hydration properties of novel films based on Saccharomyces cerevisiae biomass

Delgado, Juan Francisco; Peltzer, Mercedes Ana; Salvay, Andrés G.; Osa, O. de la; Wagner, Jorge R.

doi:10.1016/j.ifset.2018.06.017

Cited by 19 publications

(28 citation statements)

References 56 publications

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“…region has an important contribution of the curvature of the N-H bond and a small contribution of the C-N bond (Delgado et al, 2018). In this case, these modifications were most probably a consequence of protein degradation and transition of the protein to an unfolded state (Kavosi et al, 2018).…”

Section: Evolution Of Protein Profiles Of Yeast L Thermotolerans Durmentioning

confidence: 89%

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

et al. 2020

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During industrial yeast production, cells are often subjected to deleterious hydric variations during dehydration, which reduces their viability and cellular activity. This study is focused on the yeast Lachancea thermotolerans, particularly sensitive to dehydration. The aim was to understand the modifications of single-cells biophysical profiles during different dehydration conditions. Infrared spectra of individual cells were acquired before and after dehydration kinetics using synchrotron radiation-based Fourier-transform infrared (S-FTIR) microspectroscopy. The cells were previously stained with fluorescent probes in order to measure only viable and active cells prior to dehydration. In parallel, cell viability was determined using flow cytometry under identical conditions. The S-FTIR analysis indicated that cells with the lowest viability showed signs of membrane rigidification and modifications in the amide I (α-helix and β-sheet) and amide II, which are indicators of secondary protein structure conformation and degradation or disorder. Shift of symmetric C-H stretching vibration of the CH 2 group upon a higher wavenumber correlated with better cell viability, suggesting a role of plasma membrane fluidity. This was the first time that the biophysical responses of L. thermotolerans single-cells to dehydration were explored with S-FTIR. These findings are important for clarifying the mechanisms of microbial resistance to stress in order to improve the viability of sensitive yeasts during dehydration.

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Section: Evolution Of Protein Profiles Of Yeast L Thermotolerans Durmentioning

confidence: 89%

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

et al. 2020

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“…Regarding environmental issues, yeasts can be used for bioremediation and as heavy metals removers from waste waters; in agriculture, they are used as biocontrol agents; in pharmaceutical applications, for the production of several chemicals, and for biofuel production, among others [40,42,43]. A very recent and innovative application is the formation of films and coatings in the materials industry, mainly for food packaging [30].…”

Section: Yeast Biomass As Source Of Biobased Materialsmentioning

confidence: 99%

“…There are few investigations related to the use of the integral biomass to prepare biodegradable materials. Delgado et al prepared casted films based on integral yeast biomass [30,48]. In their work, the authors demonstrated that it was necessary to make a dispersion of yeast biomass (10% wt dry mass) from which the film will be formed.…”

Section: Use Of Saccharomyces Cerevisiae Cells As Film Forming Materialsmentioning

confidence: 99%

“…Then the solvent (water in this case) must be removed, favoring the interaction between the polymeric chains and the formation of the film matrix [26]. In the investigations about yeast films, glycerol was added as a plasticizer to the already treated yeast dispersion to produce casted films [30]. Yeast films were fully characterized in their visual, thermal, infrared spectroscopy (FTIR), mechanical, and hydration properties.…”

Section: Use Of Saccharomyces Cerevisiae Cells As Film Forming Materialsmentioning

confidence: 99%

“…Indeed, the cells of microorganisms (microbial biomass), for example of yeast and filamentous fungi biomass, are composed mainly of biopolymers such as proteins and polysaccharides (β-glucan) [26][27][28]. Yeast biomass, in addition to its conventional applications in fermentation processes, could be used in alternative applications such as encapsulation of compounds [29], films and coatings [30,31], among other applications. Filamentous fungi biomass is mainly used for human consumption, but fungal enzymes and bioactive compounds are widely used in the food industry and in veterinary and human medicine [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Biobased Materials from Microbial Biomass and Its Derivatives

et al. 2020

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There is a strong public concern about plastic waste, which promotes the development of new biobased materials. The benefit of using microbial biomass for new developments is that it is a completely renewable source of polymers, which is not limited to climate conditions or may cause deforestation, as biopolymers come from vegetal biomass. The present review is focused on the use of microbial biomass and its derivatives as sources of biopolymers to form new materials. Yeast and fungal biomass are low-cost and abundant sources of biopolymers with high promising properties for the development of biodegradable materials, while milk and water kefir grains, composed by kefiran and dextran, respectively, produce films with very good optical and mechanical properties. The reasons for considering microbial cellulose as an attractive biobased material are the conformational structure and enhanced properties compared to plant cellulose. Kombucha tea, a probiotic fermented sparkling beverage, produces a floating membrane that has been identified as bacterial cellulose as a side stream during this fermentation. The results shown in this review demonstrated the good performance of microbial biomass to form new materials, with enhanced functional properties for different applications.

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Impact of the film‐forming dispersion pH on the properties of yeast biomass films

et al. 2021

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BACKGROUND: Yeast biomass, mainly composed of proteins and polysaccharides (mannans and ⊎-glucans), has been proposed to develop films. pH can affect the solubility of polysaccharides, the structure of the cell wall, and the interactions between proteins. Considering the potential impact of these effects, the pH of yeast film-forming dispersions was studied from 4 to 11. RESULTS: In tensile tests, samples increased their elongation by increasing pH, from 7 ± 2% (pH 4) to 29 ± 5% (pH 11), but Young's modulus was not significantly modified. Regarding thermal degradation, the maximum degradation rate temperature was shifted 46°C from pH 4 to 11. Differences in water vapour permeability, colour, opacity, and roughness of films were also found. According to the results of differential protein solubility assay, hydrophobic interactions and hydrogen bonding were promoted at pH 4, but disulfide bonds were benefited at pH 11, in addition to partial ⊎-glucan dissolution and break-up of the alkali-sensitive linkage in molecules from the cell wall. CONCLUSION:The results lead to the conclusion that film-functional characteristics were greatly benefited at pH 11 in comparison with the regular pH of dispersion (pH 6). These results could help in understanding and selecting the pH conditions to enhance the desired properties of yeast biomass films.

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Characterization of thermal, mechanical and hydration properties of novel films based on Saccharomyces cerevisiae biomass

Cited by 19 publications

References 56 publications

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

Biophysical Stress Responses of the Yeast Lachancea thermotolerans During Dehydration Using Synchrotron-FTIR Microspectroscopy

Biobased Materials from Microbial Biomass and Its Derivatives

Impact of the film‐forming dispersion pH on the properties of yeast biomass films

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