Characterization of the Wheat Heat Shock Factor TaHsfA2e-5D Conferring Heat and Drought Tolerance in Arabidopsis

Bi, Huihui; Miao, Jingnan; He, Jinqiu; Chen, Qifan; Qian, Jiajun; Li, Huanhuan; Xu, Yan; Ma, Dan; Zhao, Yue; Tian, Xiaohong; Liu, Wenxuan

doi:10.3390/ijms23052784

Cited by 21 publications

(21 citation statements)

References 43 publications

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“…Indeed, TaHsfA2-13 was found to exhibit transcriptional activation activity in this study (Figure 3A). Similarly, in a recent study, the AHA motif was found to be important for the transcriptional activity of TaHsfA2e-5D (Bi et al, 2022). Although NLS and NES coexist, TaHsfA2-13 is normally located in the nucleus (Figures 1, 3B), which differs its orthologue from Arabidopsis (Evrard et al, 2013).…”

Section: Discussionsupporting

confidence: 64%

“…After surface disinfection with 0.1% HgCl 2 , wheat seeds were cleaned with sterile water and soaked at room temperature for 12 h. When the germ grew to 1–2 cm, seedlings were transplanted and cultivated in Hoagland nutrient solution under day/night conditions of 16 h/8 h (70–100 μmol m −2 S −1 ), 25°C, and 50–60% relative humidity. The stress treatments were performed as described by previous studies ( Duan et al, 2019 ; Liu et al, 2020 ; Bi et al, 2022 ). Seedlings at the two-leaf and one-heart stage were treated with high temperature (37°C), low temperature (4°C), H 2 O 2 (10 mmol L −1 ), mannitol (15% W/V), NaCl (0.2 mol L −1 ), MeJA (0.1 mmol L −1 ), ABA (0.2 mmol L −1 ) and SA (0.8 mmol L −1 ) for different time, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, transgenic Arabidopsis plants overexpressing TaHsfA2d showed considerable tolerance to salinity and drought stresses ( Chauhan et al, 2013 ). More recently, TaHsfA2e-5D has been shown to positively regulate heat and drought stress, at least in part by promoting the expression of stress-responsive genes ( Bi et al, 2022 ). It seems that plant Hsfs may act as the intersection of multiple signaling pathways, on the one hand participating in the regulation of abiotic signaling pathways and on the other hand mediating phytohormone signaling ( Guo et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses

Meng

Zhao

et al. 2022

Front. Plant Sci.

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Heat shock transcription factor (Hsf) exists widely in eukaryotes and responds to various abiotic stresses by regulating the expression of downstream transcription factors, functional enzymes, and molecular chaperones. In this study, TaHsfA2-13, a heat shock transcription factor belonging to A2 subclass, was cloned from wheat (Triticum aestivum) and its function was analyzed. TaHsfA2-13 encodes a protein containing 368 amino acids and has the basic characteristics of Hsfs. Multiple sequence alignment analysis showed that TaHsfA2-13 protein had the highest similarity with TdHsfA2c-like protein from Triticum dicoccoides, which reached 100%. The analysis of tissue expression characteristics revealed that TaHsfA2-13 was highly expressed in root, shoot, and leaf during the seedling stage of wheat. The expression of TaHsfA2-13 could be upregulated by heat stress, low temperature, H2O2, mannitol, salinity and multiple phytohormones. The TaHsfA2-13 protein was located in the nucleus under the normal growth conditions and showed a transcriptional activation activity in yeast. Further studies found that overexpression of TaHsfA2-13 in Arabidopsis thaliana Col-0 or athsfa2 mutant results in improved tolerance to heat stress, H2O2, SA and mannitol by regulating the expression of multiple heat shock protein (Hsp) genes. In summary, our study identified TaHsfA2-13 from wheat, revealed its regulatory function in varieties of abiotic stresses, and will provide a new target gene to improve stress tolerance for wheat breeding.

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Section: Discussionsupporting

confidence: 64%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses

Meng

Zhao

et al. 2022

Front. Plant Sci.

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“…These HSPs are extremely important for maintaining the structure of proteins. In reaction to abiotic stress, the HSPs are principally in charge of unraveling some folded proteins and preventing protein denaturation [8]. Transcription factors that regulate and provide an adaptive response under drought stress include myeloblastosis, dehydration-responsive element binding, C-repeat binding factor, abscisic acid responsive elements binding factor, ABRE binding, NAM, ATAF1/2, and CUC2 protein-containing proteins (NAC), WRKY, and SNF1-related kinase 2.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of seven different wheat cultivars for their resistance to drought in terms of growth indicators and yield

AL-Fatlawi¹,

Farhood²,

Mahdi³

et al. 2022

J App Biol Biotech

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The study used a randomized complete block design with three replicates to investigate drought tolerance genes and wheat growth and yield. The experiment included seven wheat cultivars: Buhooth 158, Rasheed, Buhooth 22, Tahade, Fatih, N70, and Cimmyt 72 in main plots and three drought treatments: Irrigation after draining 50% of available water (D1), irrigation after draining 65% of available water (D2), and irrigation after draining 75% of available water (D3) in subplots. Drought treatments resulted in an increase in the expression of genes that promote drought resistance. It is also noted that the Buhooth 158 cultivar was superior by giving the highest relative expression of LOC100382183 and LOC103630223 gene, with averages of 2.41 and 2.25 times higher than the rest of the cultivars, and this leads us to think that the Buhooth 158 cultivar has a higher drought tolerance than the rest of the cultivars. It was found that the drought treatment (D3) had a significant impact on plant height and flag leaf area, as well as on the number of tillers, spikes, and grains in a spike. The decreases in these indicators were 12.76% for plant height, 25.89% for flag leaf area; 44.00% for tillers number; 49.31% for spike number; 40.00% for grain number; and 32.21% for the weight of 1000 grains, 43.55% for grain yield, 4.91% for biological yield, and 40.44% for harvest index. Cultivars Buhooth158, Rasheed, and Buhooth 22 surpassed the rest, with the highest average yield components, grain yield, biological yield, and harvest index all indicating their superior performance.

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“…ZmHSF08 has been shown to negatively regulate several stress/ABA-responsive genes under salt and drought stress conditions in maize ( Wang et al., 2021 ). The expression of TaHSFA2e-5D is highly upregulated in wheat seedlings by heat, cold, drought, high salinity, and multiple phytohormones ( Bi et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

The heat shock factor GhHSFA4a positively regulates cotton resistance to Verticillium dahliae

et al. 2022

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Heat shock factors (HSFs) play a crucial role in the environmental stress responses of numerous plant species, including defense responses to pathogens; however, their role in cotton resistance to Verticillium dahliae remains unclear. We have previously identified several differentially expressed genes (DEGs) in Arabidopsis thaliana after inoculation with V. dahliae. Here, we discovered that GhHSFA4a in Gossypium hirsutum (cotton) after inoculation with V. dahliae shares a high identity with a DEG in A. thaliana in response to V. dahliae infection. Quantitative real-time PCR (qRT-PCR) analysis indicated that GhHSFA4a expression was rapidly induced by V. dahliae and ubiquitous in cotton roots, stems, and leaves. In a localization analysis using transient expression, GhHSFA4a was shown to be localized to the nucleus. Virus-induced gene silencing (VIGS) revealed that downregulation of GhHSFA4a significantly increased cotton susceptibility to V. dahliae. To investigate GhHSFA4a-mediated defense, 814 DEGs were identified between GhHSFA4a-silenced plants and controls using comparative RNA-seq analysis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that DEGs were enriched in “flavonoid biosynthesis”, “sesquiterpenoid and triterpenoid biosynthesis”, “linoleic acid metabolism” and “alpha-linolenic acid metabolism”. The expression levels of marker genes for these four pathways were triggered after inoculation with V. dahliae. Moreover, GhHSFA4a-overexpressing lines of A. thaliana displayed enhanced resistance against V. dahliae compared to that of the wild type. These results indicate that GhHSFA4a is involved in the synthesis of secondary metabolites and signal transduction, which are indispensable for innate immunity against V. dahliae in cotton.

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Characterization of the Wheat Heat Shock Factor TaHsfA2e-5D Conferring Heat and Drought Tolerance in Arabidopsis

Cited by 21 publications

References 43 publications

Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses

Characteristics and Regulating Roles of Wheat TaHsfA2-13 in Abiotic Stresses

Evaluation of seven different wheat cultivars for their resistance to drought in terms of growth indicators and yield

The heat shock factor GhHSFA4a positively regulates cotton resistance to Verticillium dahliae

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