Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven

Correa-Llantén, Daniela N.; Larrain-Linton, Juanita; Muñoz, Patricio A.; Castro, Miguel E.; Boehmwald, Freddy; Blamey, Jenny M.

doi:10.4014/kjmb.1301.01004

Cited by 6 publications

(6 citation statements)

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“…Size distribution ranged from 40 to 160 nm, where 70 % of nanoparticles have less than 100 nm in size. The size of all nanoparticles was less than 100 nm at pH 4.0; over 50 % of nanoparticles have less than 100 nm at pH 5.0; at pH 6.0 and 8.0 over 90 % of nanoparticles have less than 100 nm in size (Correa-Llantén et al 2014). At neutral pH (7.0) nanoparticles reach a size around 120 nm and only 20 % of them were less than 100 nm.…”

Section: Geobacillus Wiegelii Strain Gwe1mentioning

confidence: 88%

“…At neutral pH (7.0) nanoparticles reach a size around 120 nm and only 20 % of them were less than 100 nm. Nanoparticles did not show a significant difference in size when they were incubated between 0 and 3 h at 60 °C (Correa-Llantén et al 2014). Meanwhile at 80 °C the nanoparticles suspension lost its homogeneity.…”

Section: Geobacillus Wiegelii Strain Gwe1mentioning

confidence: 96%

“…Geobacillus wiegelii, strain GWE1, an aerobic thermophile belonging to genus Geobacillus, isolated from a drying oven (Correa-Llantén et al 2014). This thermophile has the ability to reduce selenite evidenced by the change of color from colorless to red in the culture (Fig.…”

Section: Geobacillus Wiegelii Strain Gwe1mentioning

confidence: 99%

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Thermophiles and Psychrophiles in Nanotechnology

Tiquia-Arashiro

Rodrigues

2016

Extremophiles: Applications in Nanotechnology

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Some thermophiles and psychrophiles have developed the ability to resort to specific defense mechanisms to quell stresses like toxicity of heavy metal ions or metals. Some of them could survive and grow even at high metal ion concentrations and are capable of binding large quantities of metallic cations. Moreover, some of these microorganisms are able to synthesize nanoparticles. The remarkable ability of these group of microbes to reduce heavy metal ions make them one of the best candidates for nanoparticle synthesis. In this chapter, thermophilic and psychrophilic microorganisms used in nanoparticle biosynthesis are presented. The aim of chapter is to make a reflection on the current state and future prospects and especially the possibilities and limitations of the use of extremophiles in bio-based technique for industries.

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Section: Geobacillus Wiegelii Strain Gwe1mentioning

confidence: 88%

Section: Geobacillus Wiegelii Strain Gwe1mentioning

confidence: 96%

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Thermophiles and Psychrophiles in Nanotechnology

Tiquia-Arashiro

Rodrigues

2016

Extremophiles: Applications in Nanotechnology

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“…For instance, Deinococcus radiodurans , a radioresistant, thermotolerant, psychrotolerant and acid‐tolerant bacterium, has been shown to produce protein‐capped spherical silver NPs (Kulkarni et al, 2015 ). Another example is the thermophilic bacterium Geobacillus wiegelii strain GWE1, which produces SeNPs and TeNPs both intracellularly and extracellularly, and whose size and shape have been shown to be modulated by pH and temperature (Correa‐Llantén et al, 2014 ).…”

Section: Searching For the Bacterial Chassis For Menps ...mentioning

confidence: 99%

Opportunities and obstacles in microbial synthesis of metal nanoparticles

Carmona

Poblete‐Castro

Rai

et al. 2023

Microbial Biotechnology

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Metallic nanoparticles (MeNPs) are widely used in many areas such as biomedicine, packaging, cosmetics, colourants, agriculture, antimicrobial agents, cleaning products, as components of electronic devices and nutritional supplements. In addition, some MeNPs exhibit quantum properties, making them suitable materials in the photonics, electronic and energy industries. Through the lens of technology, microbes can be considered nanofactories capable of producing enzymes, metabolites and capping materials involved in the synthesis, assembly and stabilization of MeNPs. This bioprocess is considered more ecofriendly and less energy intensive than the current chemical synthesis routes. However, microbial synthesis of MeNPs as an alternative method to the chemical synthesis of nanomaterials still faces some challenges that need to be solved. Some of these challenges are described in this Editorial.

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“…Escherichia coli strain BL21 (Promega, Madison, WI, USA) and Geobacillus sp. strain GWE1 (personal culture collection) [33] were used as control microorganisms. E. coli strain BL21, strain GWE1, and strain I1P were grown in Luria-Bertani (LB) medium (at 37 • C), LB/3 medium (at 70 • C), and LB 6% NaCl (at 22 • C), respectively.…”

Section: Effect Of Uv Radiation On Cell Viabilitymentioning

confidence: 99%

Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase

Monsalves¹,

Ollivet-Besson²,

Amenábar³

et al. 2020

Microorganisms

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Microorganisms present in Antarctica have to deal not only with cold temperatures but also with other environmental conditions, such as high UV radiation, that trigger the generation of reactive oxygen species. Therefore, Antarctic microorganisms must have an important antioxidant defense system to prevent oxidative damage. One of these defenses are antioxidant enzymes, such as catalase, which is involved in the detoxification of hydrogen peroxide produced under oxidative conditions. Here, we reported the isolation and partial characterization of an Antarctic bacterium belonging to the Serratia genus that was resistant to UV-C radiation and well-adapted to cold temperatures. This microorganism, denominated strain I1P, was efficient at decreasing reactive oxygen species levels produced after UV-C irradiation. Genomic and activity assays suggested that the enzymatic antioxidant defense mechanisms of strain I1P, especially its catalase enzyme, may confer UV resistance. This catalase was active in a wide range of temperatures (20–70 °C), showing optimal activity at 50 °C (at pH 7.0), a remarkable finding considering its psychrotolerant origin. In addition, this enzyme was thermostable, retaining around 60% of its activity after 6 h of incubation at 50 °C. The antioxidant defense systems of strain I1P, including its surprisingly thermoactive and thermostable catalase enzyme, make this microorganism a good source of biocompounds with potential biotechnological applications.

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Characterization of the Thermophilic Bacterium Geobacillus sp. Strain GWE1 Isolated from a Sterilization Oven

Cited by 6 publications

References 0 publications

Thermophiles and Psychrophiles in Nanotechnology

Thermophiles and Psychrophiles in Nanotechnology

Opportunities and obstacles in microbial synthesis of metal nanoparticles

Isolation of a Psychrotolerant and UV-C-Resistant Bacterium from Elephant Island, Antarctica with a Highly Thermoactive and Thermostable Catalase

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