BackgroundThe use of microorganisms in the synthesis of nanoparticles emerges as an eco-friendly and exciting approach, for production of nanoparticles due to its low energy requirement, environmental compatibility, reduced costs of manufacture, scalability, and nanoparticle stabilization compared with the chemical synthesis.ResultsThe production of gold nanoparticles by the thermophilic bacterium Geobacillus sp. strain ID17 is reported in this study. Cells exposed to Au3+ turned from colourless into an intense purple colour. This change of colour indicates the accumulation of intracellular gold nanoparticles. Elemental analysis of particles composition was verified using TEM and EDX analysis. The intracellular localization and particles size were verified by TEM showing two different types of particles of predominant quasi-hexagonal shape with size ranging from 5–50 nm. The mayority of them were between 10‒20 nm in size. FT-IR was utilized to characterize the chemical surface of gold nanoparticles. This assay supports the idea of a protein type of compound on the surface of biosynthesized gold nanoparticles. Reductase activity involved in the synthesis of gold nanoparticles has been previously reported to be present in others microorganisms. This reduction using NADH as substrate was tested in ID17. Crude extracts of the microorganism could catalyze the NADH-dependent Au3+ reduction.ConclusionsOur results strongly suggest that the biosynthesis of gold nanoparticles by ID17 is mediated by enzymes and NADH as a cofactor for this biological transformation.

Alzheimer’s disease is a common tauopathy where fibril formation and aggregates are the hallmark of the disease. Efforts targeting amyloid-β plaques have succeeded to remove plaques but failed in clinical trials to improve cognition; thus, the current therapeutic strategy is at preventing tau aggregation. Here, we demonstrated that four phenolic diterpenoids and rosmarinic acid inhibit fibrillization. Since, rosmarinic acid was the most active compound, we observe morphological changes in atomic force microscopy images after treatment. Hence, rosmarinic acid leads to a decrease in amide regions I and III, indicating that rosmarinic acid prevents β-sheet assembly. Molecular docking study inside the steric zipper model of the hexapeptide 306VQIVYK311 involved in fibrillization and β sheet formation, suggests that rosmarinic acid binds to the steric zipper with similar chemical interactions with respect to those observed for orange G, a known pharmacofore for amyloid.

Although turbulence has been conjectured to be important for magnetic reconnection, still very little is known about its role in collisionless plasmas. Previous attempts to quantify the effect of turbulence on reconnection usually prescribed Alfvénic or other low-frequency fluctuations or investigated collisionless kinetic effects in just two-dimensional configurations and antiparallel magnetic fields. In view of this, we analyzed the kinetic turbulence self-generated by three-dimensional guidefield reconnection through force-free current sheets in frequency and wavenumber spaces, utilizing 3D particle-in-cell code numerical simulations. Our investigations reveal reconnection rates and kinetic turbulence with features similar to those obtained by current in-situ spacecraft observations of MMS as well as in the laboratory reconnection experiments MRX, VTF and Vineta-II. In particular we found that the kinetic turbulence developing in the course of 3D guide-field reconnection exhibits a broadband power-law spectrum extending beyond the lower-hybrid frequency and up to the electron frequencies. In the frequency space the spectral index of the turbulence appeared to be close to -2.8 at the reconnection X-line. In the wavenumber space it also becomes -2.8 as soon as the normalized reconnection rate reaches 0.1. The broadband kinetic turbulence is mainly due to current-streaming and electron-flow-shear instabilities excited in the sufficiently thin current sheets of kinetic reconnection. The growth of the kinetic turbulence corresponds to high reconnection rates which exceed those of fast laminar, non-turbulent reconnection.

Deception Island, an active stratovolcano located in the South Shetland Islands, Antarctica, provides excellent conditions for the thermophilic bacteria growth because of high ground temperatures in specific areas, such as Fumarole Bay where the temperatures are above the mesophilic range. Denaturing Gradient Gel Electrophoresis (DGGE) was used with the 16S ribosomal gene to analyse cultures of thermophilic bacteria from a soil sample taken from Fumarole Bay. Nine bands were sequenced and analysed from DGGE and they indicated the presence of bacteria from the genera Geobacillus, Bacillus, Brevibacillus, Thermus and uncultured sulphate reducing bacteria. Some of which have been reported in other Antarctic geothermal sites. Geobacillus, Bacillus and Brevibacillus genera were successfully cultivated in an enriched medium. A pure culture of one thermophilic Geobacillus bacterium was obtained closely related to Geobacillus jurassicus.

Non-Maxwellian electron velocity space distribution functions (EVDF) are useful signatures of plasma conditions and non-local consequences of collisionless magnetic reconnection. In the past, EVDFs were obtained mainly for antiparallel reconnection and under the influence of weak guide-fields in the direction perpendicular to the reconnection plane. EVDFs are, however, not well known, yet, for oblique (or component-) reconnection in case and in dependence on stronger guide-magnetic fields and for the exhaust (outflow) region of reconnection away from the diffusion region. In view of the multi-spacecraft Magnetospheric Multiscale Mission (MMS), we derived the non-Maxwellian EVDFs of collisionless magnetic reconnection in dependence on the guide-field strength b g from small (b g ≈ 0) to very strong (b g = 8) guide-fields, taking into account the feedback of the self-generated turbulence. For this sake, we carried out 2.5D fully-kinetic Particle-in-Cell simulations using the ACRONYM code. We obtained anisotropic EVDFs and electron beams propagating along the separatrices as well as in the exhaust region of reconnection. The beams are anisotropic with a higher temperature in the direction perpendicular rather than parallel to the local magnetic field. The beams propagate in the direction opposite to the background electrons and cause instabilities. We also obtained the guide-field dependence of the relative electron-beam drift speed, threshold and properties of the resulting streaming instabilities including the strongly non-linear saturation of the self-generated plasma turbulence. This turbulence and its non-linear feedback cause non-adiabatic parallel electron acceleration. We further obtained the resulting EVDFs due to the non-linear feedback of the saturated self-generated turbulence near the separatrices and in the exhaust region of reconnection in dependence on the guide field strength. We found that the influence of the self-generated plasma turbulence leads well beyond the limits of the quasi-linear approximation, to the creation of phase space holes and an isotropizing pitch-angle scattering. EVDFs obtained by this way can be used for diagnosing collisionless reconnection by using the multi-spacecraft observations carried out by the MMS mission.

BackgroundAntifreeze proteins (AFPs) production is a survival strategy of psychrophiles in ice. These proteins have potential in frozen food industry avoiding the damage in the structure of animal or vegetal foods. Moreover, there is not much information regarding the interaction of Antarctic bacterial AFPs with ice, and new determinations are needed to understand the behaviour of these proteins at the water/ice interface.ResultsDifferent Antarctic places were screened for antifreeze activity and microorganisms were selected for the presence of thermal hysteresis in their crude extracts. Isolates GU1.7.1, GU3.1.1, and AFP5.1 showed higher thermal hysteresis and were characterized using a polyphasic approach. Studies using cucumber and zucchini samples showed cellular protection when samples were treated with partially purified AFPs or a commercial AFP as was determined using toluidine blue O and neutral red staining. Additionally, genome analysis of these isolates revealed the presence of genes that encode for putative AFPs. Deduced amino acids sequences from GU3.1.1 (gu3A and gu3B) and AFP5.1 (afp5A) showed high similarity to reported AFPs which crystal structures are solved, allowing then generating homology models. Modelled proteins showed a triangular prism form similar to β-helix AFPs with a linear distribution of threonine residues at one side of the prism that could correspond to the putative ice binding side. The statistically best models were used to build a protein-water system. Molecular dynamics simulations were then performed to compare the antifreezing behaviour of these AFPs at the ice/water interface. Docking and molecular dynamics simulations revealed that gu3B could have the most efficient antifreezing behavior, but gu3A could have a higher affinity for ice.ConclusionsAFPs from Antarctic microorganisms GU1.7.1, GU3.1.1 and AFP5.1 protect cellular structures of frozen food showing a potential for frozen food industry. Modeled proteins possess a β-helix structure, and molecular docking analysis revealed the AFP gu3B could be the most efficient AFPs in order to avoid the formation of ice crystals, even when gu3A has a higher affinity for ice. By determining the interaction of AFPs at the ice/water interface, it will be possible to understand the process of adaptation of psychrophilic bacteria to Antarctic ice.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0737-2) contains supplementary material, which is available to authorized users.

This study aimed to characterize the in vitro antioxidant and antibacterial properties of oregano (Origanum vulgare) essential oil, as well as its chemical composition. To our best knowledge, there are few studies on oregano grown in the arid Andes region, but none on the metabolites produced and their bioactivity. This work identified fifty metabolites by Gas Chromatography–Mass Spectrometry (GC-MS)—monoterpene hydrocarbons, oxygenated monoterpenes, phenolic monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenes—present in the essential oil of oregano collected in the Atacama Desert. The main components of essential oregano oil were thymol (15.9%), Z-sabinene hydrate (13.4%), γ-terpinene (10.6%), p-cymene (8.6%), linalyl acetate (7.2%), sabinene (6.5%), and carvacrol methyl ether (5.6%). The antibacterial tests showed that the pathogenic bacteria Staphylococcus aureus and Salmonella enterica and the phytopathogenic bacteria Erwinia rhapontici and Xanthomonas campestris were the most susceptible to oregano oil, with the lowest concentrations of oil necessary to inhibit their bacterial growth. Moreover, oregano oil showed antibacterial activity against bacteria associated with food poisoning. In conclusion, O. vulgare from the arid Andean region possesses an important antibacterial activity with a high potential in the food industry and agriculture.

We present a new hybrid algorithm implemented in the code CHIEF ( Code Hybrid with Inertial Electron Fluid) for simulations of electron–ion plasmas. The algorithm treats the ions kinetically, modeled by the Particle-in-Cell (PiC) method, and electrons as an inertial fluid, modeled by electron fluid equations without any of the approximations used in most of the other hybrid codes with an inertial electron fluid. This kind of code is appropriate to model a large variety of quasineutral plasma phenomena where the electron inertia and/or ion kinetic effects are relevant. We present here the governing equations of the model, how these are discretized and implemented numerically, as well as six test problems to validate our numerical approach. Our chosen test problems, where the electron inertia and ion kinetic effects play the essential role, are: 0) Excitation of parallel eigenmodes to check numerical convergence and stability, 1) parallel (to a background magnetic field) propagating electromagnetic waves, 2) perpendicular propagating electrostatic waves (ion Bernstein modes), 3) ion beam right-hand instability (resonant and non-resonant), 4) ion Landau damping, 5) ion firehose instability, and 6) 2D oblique ion firehose instability. Our results reproduce successfully the predictions of linear and non-linear theory for all these problems, validating our code. All properties of this hybrid code make it ideal to study multi-scale phenomena between electron and ion scales such as collisionless shocks, magnetic reconnection and kinetic plasma turbulence in the dissipation range above the electron scales