Characterization of the T cell response to the major hazelnut allergen, Cor a 1.04: evidence for a relevant T cell epitope not cross‐reactive with homologous pollen allergens

Bohle, Barbara; Radakovics, Astrid; Lüttkopf, D.; Jahn‐Schmid, Beatrice; Vieths, Stefan; Ebner, Christof

doi:10.1111/j.1365-2222.2005.02332.x

Cited by 47 publications

(43 citation statements)

References 45 publications

(80 reference statements)

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“…1). The same region had already previously attracted our attention as the majority of Cor a 1‐specific TCC reactive to epitopes located within aa 142–153 cross‐reacted with Dau c 1 but not with Bet v 1 22. In the present study, all seven Cor a 1‐reactive Dau c 1‐specific TCL harbored T cells specific for epitopes within aa 142–153 and four of them did not react to Bet v 1.…”

Section: Discussionsupporting

confidence: 73%

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Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

Zulehner

Nagl

Briza

et al. 2016

Allergy

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BackgroundIn contrast to other Bet v 1‐related food allergens, the major carrot allergen, Dau c 1, has been suggested to induce food allergy independently from Bet v 1. As T cells are crucial in the sensitization process, we sought to characterize the T‐cell response to Dau c 1 and its cross‐reactivity with Bet v 1.MethodsDau c 1‐specific T‐cell lines (TCL) and clones (TCC) established from PBMC of birch pollen‐allergic patients with carrot allergy were analyzed for reactivity to Bet v 1, epitope specificity, allergen‐induced cytokine secretion, and expression of integrins α4β7 and α4β1, critical for gut and lung homing, respectively. mRNA expression of GATA3 and Tbet was analyzed in sorted CD3+ CD4+ CFSE low cells proliferating upon stimulation of PBMC with Dau c 1 or Bet v 1. Dau c 1 was incubated with endolysosomal proteases, and the resulting fragments were identified by mass spectrometry.ResultsAmong 14 distinct T‐cell‐activating regions, Dau c 1139–153 was recognized by 55% of the patients. Only 6 of 15 (40%) Dau c 1‐specific TCL and 9 of 21 (43%) TCC reacted with Bet v 1. Bet v 1‐nonreactive TCC were mainly Th1‐like and showed a higher expression of the integrin β7 and a significantly lower expression of the integrin β1 than Bet v 1‐positive TCC. A Th1‐like response was also detected in Dau c 1‐reactive CD3+ CD4+ CFSE low cells. Full‐length Dau c 1 was still detectable after 48 h of endolysosomal degradation. Proteolytic fragments of Dau c 1 matched its T‐cell‐activating regions.ConclusionDau c 1 displays several characteristics of sensitizing allergens, namely a major T‐cell‐activating region, low susceptibility to endolysosomal degradation, and induction of a Bet v 1‐independent T‐cell response. These cellular insights confirm that the major carrot allergen has a special status among Bet v 1‐related food allergens.

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Section: Discussionsupporting

confidence: 73%

“…In the past, we and others have characterized the T‐cell response to Api g 1, Cor a 1, and Mal d 1, and their cellular cross‐reactivity with the major birch pollen allergen 4, 11, 21, 22, 23, 24. These studies have shown that T lymphocytes reactive with these food allergens are mainly cross‐reactive Bet v 1‐specific T cells.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

Zulehner

Nagl

Briza

et al. 2016

Allergy

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“…3, Tables II, III). This finding was unexpected, because, for example, the food allergens homologous to the major birch pollen allergen, Bet v 1, all possess multiple epitope regions similar to Bet v 1 (24,25), and T cell cross stimulation has been observed for epitopes with 60-80% amino acid similarity and 40-67% amino acid identity (26). For Amb a 1, we have previously found .20 distinct T cell epitope regions, including 3 dominant epitopes (Amb a 1 178-189 , Amb a 1 199-216 , and Amb a 1 343-357 ) recognized by .45%, and 7 epitopes recognized by .25% of patients (22).…”

Section: Discussionmentioning

confidence: 80%

Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Jahn‐Schmid

Hauser

Wopfner

et al. 2012

The Journal of Immunology

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Ragweed and mugwort are closely related weeds that represent the major cause of pollen allergy in late summer. Concomitant sensitization and clinical cross-reactivity frequently occur in subjects who are coexposed to both pollen species, and have implications for diagnosis and specific immunotherapy. Molecules involved in this cross-reactivity might be Amb a 1, the major ragweed pollen allergen, and Art v 6, a highly homologous allergen from mugwort. Therefore, we investigated the IgE and T cell response to Art v 6 of 60 weed pollen-allergic patients and assessed its immunological cross-reactivity with Amb a 1. Results of ELISA inhibition experiments suggested that both allergens are largely cross-reactive, but Amb a 1 possesses more IgE epitopes than Art v 6. In patients with IgE to both allergens, Amb a 1-induced T cell lines and clones responded weakly to Art v 6. Moreover, Art v 6-induced T cell lines responded stronger to Amb a 1. T cell epitope mapping of Art v 6 revealed that it contains only a few cross-reactive epitopes, which is opposed to the multiple T cell-activating regions present in Amb a 1. In summary, Amb a 1 can elicit more diverse allergen-specific IgE and T cell responses than Art v 6 and dominates the cross-reactivity with its homolog. Nevertheless, Art v 6 can act as a primary sensitizing allergen in areas with high mugwort pollen exposure, and consequently may facilitate sensitization to Amb a 1 by epitope cross-recognition of T and B cells.

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“…Pru-p-3-specific TCLs with an IL-4/IFN-γ ratio of >5 were characterized as having a Th2 profile. In contrast, an IFN-γ/IL-4 ratio of >5 indicated a Th1 profile; similar or undetectable levels of both of these cytokines indicated a Th0 profile [28]. To confirm their Th profile, we analyzed those TCLs that responded to a single peptide.…”

Section: Resultsmentioning

confidence: 99%

“…TCLs releasing at least 5 times more IL-4 than IFN-γ were classified as Th2 TCLs, whereas those releasing at least 5 times more IFN-γ than IL-4 were classified as Th1 TCLs. TCLs producing similar levels of IL-4 and IFN-γ were classified as Th0 [28]. …”

Section: Methodsmentioning

confidence: 99%

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

Pastorello

Monza

Pravettoni

et al. 2010

Int Arch Allergy Immunol

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Background: Pru p 3 is the major peach allergen recognized by more than 90% of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≧5). These TCLs proliferated mainly in response to Pru p 3_12–27 and Pru p 3_57–72. Pru-p-3-specific TCLs were mainly CD4+ (81%) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 3_12–27 and Pru p 3_57–72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.

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Characterization of the T cell response to the major hazelnut allergen, Cor a 1.04: evidence for a relevant T cell epitope not cross‐reactive with homologous pollen allergens

Cited by 47 publications

References 45 publications

Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

Characterization of the T-cell response to Dau c 1, the Bet v 1-homolog in carrot

Humoral and Cellular Cross-Reactivity between Amb a 1, the Major Ragweed Pollen Allergen, and Its Mugwort Homolog Art v 6

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

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