Characterization of the T-cell subpopulations in the granulation tissues of chronic suppurative otitis media

Abstract

The present study aimed to investigate the potential involvement of specific T-cell subpopulations in granulation tissue formation in chronic suppurative otitis media (CSOM). Fifteen patients with CSOM were enrolled in this study. Granulation tissues were obtained from the middle ear cavity. Hematoxylin and eosin staining was performed for histopathological observation, and different T-cell subpopulations were characterized by immunohistochemistry. No evident association was identified between granulation tissue formation and disease course. The number of cluster of differentiation 8+ (CD8+) T cells, forkhead box P3+ (FOXP3+) regulatory T (Treg) cells and OX40+ T cells were significantly higher in granulation tissues from patients with ear discharge within the last 6 months compared to those without (P<0.05). Fresh granulation tissues had more CD8+ T cells and FOXP3+ Treg cells compared to the mature granulation tissues (P<0.05). There was a differential abundance of specific T-cell subpopulations in the granulation tissues in CSOM with different disease courses or with ear discharge, suggesting that T cell-mediated cellular immunity is involved in lesion formation of CSOM.

Interaction Between Regulatory T Cells and Antibody-Producing B Cells for Immune Responses at the Upper Respiratory Mucosa Against NontypeableHaemophilus influenzae: In Vitro Assay Model

“…6 In fact, it has been reported that Tregs accumulate in the middle ear cavity and nasal mucosa in patients with otitis media and rhinosinusitis. 7,8 Even in a mouse model of otitis media induced by NTHi, Tregs accumulated in the middle ear mucosa. 9 On the basis of these reports, Tregs may affect mucosal immune responses against pathogens.…”

Acoustic and Speech Perceptual Evaluation of health related Quality of Life among adults with Chronic Suppurative Otitis Media

“…World Health Organization (WHO) label the individual as patient of CSOM just after 14 days of ear discharge however, otolaryngologists wait for more than three months for the same condition. Clinically, the chronic suppurative otitis media manifests as noteworthy practical restrictions of hearing.7.8.9 In unindustrialized republics, infections of ear and hearing loss are habitually mistreated complaint owing to work force, insufficient funds, knowledge and facilities.10 However, by advances in hygiene, housing, and use of antibiotic in industrialized republics, the frequency of chronic suppurative otitis media has considerably reduced but it still signifies a hazardous a i l m e n t w i t h s e v e r e d i f f i c u l t i e s l i k e 11,12,13 cholesteatoma.…”