Characterization of the structural changes of human serum albumin upon interaction with single-walled and multi-walled carbon nanotubes: spectroscopic and molecular modeling approaches

Hosseinzadeh, Maral; Nikjoo, Shadi; Zare, Najme; Delavar, Delaram; Beigoli, Sima; Chamani, Jamshidkhan

doi:10.1007/s11164-018-3608-5

Cited by 98 publications

(29 citation statements)

References 63 publications

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“…The binding constant of WP‐ZnO NPs complex at 298 K is more than the binding constant at 310 K. It means that temperature reduces the binding affinity of ZnO NPs to WP. In the static quenching, K b decreases with the increase of temperature (Hosseinzadeh et al., 2019). Dynamic quenching is the opposite.…”

Section: Resultsmentioning

confidence: 99%

Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Yuan

Zhou

Nie

et al. 2022

Journal of Food Science

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The interaction between zinc oxide nanoparticles (ZnO NPs) and whey protein (WP) was studied. The gastric epithelial cell line (GES‐1) was used to evaluate the toxicity intensity of ZnO NPs. The interaction mechanism of ZnO NPs and WP was studied by spectroscopic techniques. The results showed that the inhibitory effect of ZnO NPs on cells activity could be reduced when added to ZnO NPs at a concentration of 50 µg/ml. The fluorescence quenching mechanism of ZnO NPs on WP is a combination of dynamic and static quenching. The interaction force between ZnO NPs and WP can be considered as H‐bond and VdW force, and they have two binding sites. The interaction between WP and ZnO NPs leads to the loosening of the structural skeleton of WP and the extension of peptide chain, which exposes the tyrosine (Tyr) and tryptophan (Trp) hydrophobic groups in the hydrophobic region of protein molecules and reduces the hydrophobicity of the microenvironment. The ZnO NPs might form a complex with WP through H‐bond, hydrophobic interactions, and so on, leading to peptide chain rearrangement, and finally causing changes in the secondary structure of α‐helix. Practical ApplicationThis study provides a theoretical basis for future research on the interaction between food ingredients and nanomaterials, the evaluation of toxicity of nanomaterials and the application scope of nanomaterials in food field.

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Section: Resultsmentioning

confidence: 99%

Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Yuan

Zhou

Nie

et al. 2022

Journal of Food Science

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“…Drug and DNA can bind in vitro , and they can also bind to each other successfully in vivo . [ 28 ] The K a values were both in the order of 10 3 for the tiamulin–DNA complex from the two calculation methods, showing a stable binding affinity. Clinical dosages can be assessed according to binding affinity.…”

Section: Resultsmentioning

confidence: 99%

Exploring the intercalation binding of tiamulin with DNA using multispectroscopy and a molecular modelling approach

Wang

et al. 2022

Luminescence

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The binding of tiamulin with calf thymus DNA was systematically investigated using multispectroscopy and molecular modelling techniques. For DNA, once tiamulin was added, viscosity (η) and melting temperature (Tm) both exhibited an uptrend. The fluorescence performance of the tiamulin–DNA complex did not change with the ionic strength changes. The binding constant (Ka) of tiamulin for single‐stranded DNA (ssDNA, 1.48 × 104 M−1) was obviously higher than that for double‐stranded DNA (dsDNA, 9.51 × 103 M−1) at 291 K. The helix structure became looser and the base stack force became stronger for DNA due to the presence of tiamulin as seen from circular dichroic (CD) spectra. The intercalation binding mode of tiamulin with DNA was disclosed. Molecular modelling also revealed tiamulin inserting into the base pairs with the lowest binding free energy of −18.73 kJ mol−1 using van der Waals forces as well as hydrogen bonds.

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“…CNTs were oxidized using H 2 SO 4 /HNO 3 under the ultrasonic condition to provide COOH-CNTs. In this process, the phosphate buffer was used to dissolve HSA, which was added to COOH-CNTs solution to obtain HSA-CNTs [51]. HSA can facilitate binding and carrying a lot of poorly soluble antibiotic drugs like bendroflumethiazide in water [52].…”

Section: Human Serum Albumin (Hsa)-cntsmentioning

confidence: 99%

The role of carbon nanotubes in antibiotics drug delivery

Ziarani¹,

Ashtiani²,

Mohajer³

et al. 2021

Frontiers Drug Chemistry Clinical Res

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Characterization of the structural changes of human serum albumin upon interaction with single-walled and multi-walled carbon nanotubes: spectroscopic and molecular modeling approaches

Cited by 98 publications

References 63 publications

Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Exploring the intercalation binding of tiamulin with DNA using multispectroscopy and a molecular modelling approach

The role of carbon nanotubes in antibiotics drug delivery

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