Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Yuan, Erdong; Zhou, Miao; Nie, Shiying; Ren, Jiaoyan

doi:10.1111/1750-3841.16193

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…In our study, in some cases, alum was found to be more effective than SFNPs, which could be due to the formation of antigen depots that gradually release antigen [25] or due to the impact of nanoparticles encapsulation (SFNPs) on protein conformation. [26,27] In addition, the previous studies have reported enhanced antibody responses with alum, [13,28] whereas our study showed a significant Th1 response that can be due to the presence of immunodominant T-cell epitopes in the designed multi-epitope protein.…”

Section: Discussioncontrasting

confidence: 59%

Immunogenic Potential and Therapeutic Efficacy of Multi‐Epitope Encapsulated Silk Fibroin Nanoparticles against Pseudomonas aeruginosa‐Mediated Urinary Tract Infections

Rezvanirad

Habibi

Farokhi

et al. 2023

Macromolecular Bioscience

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Pseudomonas aeruginosa (P. aeruginosa) causing urinary tract infections (UTIs) are a major concern among hospital‐acquired infections. The need for an effective vaccine that reduces the infections is imperative. This study aims to evaluate the efficacy of a multi‐epitope vaccine encapsulated in silk fibroin nanoparticles (SFNPs) against P. aeruginosa‐mediated UTIs. A multi‐epitope is constructed from nine proteins of P. aeruginosa using immunoinformatic analysis, expressed, and purified in BL21 (DE3) cells. The encapsulation efficiency of the multi‐epitope in SFNPs is 85% with a mean particle size of 130 nm and 24% of the encapsulated antigen is released after 35 days. The vaccine formulations adjuvanted with SFNPs or alum significantly improve systemic and mucosal humoral responses and the cytokine profile (IFN‐γ, IL‐4, and IL‐17) in mice. Additionally, the longevity of the IgG response is maintained for at least 110 days in a steady state. In a bladder challenge, mice treated with the multi‐epitope admixed with alum or encapsulated in SFNPs demonstrate significant protection of the bladder and kidneys against P. aeruginosa. This study highlights the promising therapeutic potential of a multi‐epitope vaccine encapsulated in SFNPs or adjuvanted with alum against P. aeruginosa infections.

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Section: Discussioncontrasting

confidence: 59%

Immunogenic Potential and Therapeutic Efficacy of Multi‐Epitope Encapsulated Silk Fibroin Nanoparticles against Pseudomonas aeruginosa‐Mediated Urinary Tract Infections

Rezvanirad

Habibi

Farokhi

et al. 2023

Macromolecular Bioscience

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“…The hydrophobic Trp residues and hydrophilic Tyr residues were the main sources of endogenous fluorescence when the excitation wavelength was 280 nm. 48 Fluorescence emission spectrum of LuxS is shown in Figure 6A , with a strong emission peak at 286 nm. With the increase of BAI concentration, the endogenous fluorescence of LuxS gradually weakened, and it shows that BAI interacts with LuxS and quenches the endogenous fluorescence of LuxS.…”

Section: Resultsmentioning

confidence: 99%

Baicalein Inhibits the Staphylococcus aureus Biofilm and the LuxS/AI-2 System in vitro

Mao¹,

Li²,

Chen³

et al. 2023

IDR

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Introduction Staphylococcus aureus ( S. aureus ) is a common cause of mastitis in dairy cows, a condition that has a significant economic impact. S. aureus displays quorum sensing (QS) system-controlled virulence characteristics, like biofilm formation, that make therapy challenging. In order to effectively combat S. aureus , one potential technique is to interfere with quorum sensing. Methods This study evaluated the effects of different Baicalin (BAI) concentrations on the growth and the biofilm of S. aureus isolates, including the biofilm formation and mature biofilm clearance. The binding activity of BAI to LuxS was verified by molecular docking and kinetic simulations. The secondary structure of LuxS in the formulations was characterized using fluorescence quenching and Fourier transform infrared (FTIR) spectroscopy. Additionally, using fluorescence quantitative PCR, the impact of BAI on the transcript levels of the luxS and biofilm-related genes was investigated. The impact of BAI on LuxS at the level of protein expression was also confirmed by a Western blotting investigation. Results According to the docking experiments, they were able to engage with the amino acid residues in LuxS and BAI through hydrogen bonding. The results of molecular dynamics simulations and the binding free energy also confirmed the stability of the complex and supported the experimental results. BAI showed weak inhibitory activity against S. aureus but significantly reduced biofilm formation and disrupted mature biofilms. BAI also downregulated luxS and biofilm-associated genes’ mRNA expression. Successful binding was confirmed using fluorescence quenching and FTIR. Discussion We thus report that BAI inhibits the S. aureus LuxS/AI-2 system for the first time, which raises the possibility that BAI could be employed as a possible antimicrobial drug to treat S. aureus strain-caused biofilms.

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“…[36,37] Furthermore, there are some evidences, indicating a beneficial interaction between proteins and ZnO. [38][39][40][41] However, the protein-assisted in situ synthesis of ZnO in NR latex remains unreported.…”

Section: Introductionmentioning

confidence: 99%

Protein‐Mediated ZnO Synthesis for Self‐Healing in Natural Rubber

Fueangphakdee,

Nimpaiboon,

Junkong

2024

Macro Materials & Eng

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Natural rubber (NR) consists of mainly cis‐1,4‐polyisoprene with proteins as notable nonrubber components that significantly influence its properties. A relatively unexplored aspect is the role of these proteins in reducing and stabilizing metal ions to form metallic particles. Here, the NR/ZnO composite is successfully prepared by in situ generation of ZnO in the NR latex for the first time. With the aid of the proteins, the amount of ZnO is slightly higher than that formed in the saponified NR latex (SPNR), where some proteins are removed. Importantly, the noncovalent bonds between the proteins and/or amino acids with the ZnO formed in the NR latex can provide the self‐healing behavior of the NR/ZnO composite. However, heating at ≈40 °C is still necessary for reducing the healing time and increasing the self‐healing efficiency of the composite. Nevertheless, greater noncovalent bonds within NR/ZnO hinder rubber molecular chain interdiffusion and rearrangement essential for self‐healing behavior in uncross‐linked rubber, resulting in lower healing efficiency compared to SPNR/ZnO. This study underscores the significance of proteins and/or amino acids on NR's properties, despite their minor presence. Without sophisticated NR modification, this is a very simple and low‐cost technique to achieve the NR/ZnO composite with self‐healing behavior.

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Interaction mechanism between ZnO nanoparticles‐whey protein and its effect on toxicity in GES‐1 cells

Cited by 7 publications

References 29 publications

Immunogenic Potential and Therapeutic Efficacy of Multi‐Epitope Encapsulated Silk Fibroin Nanoparticles against Pseudomonas aeruginosa‐Mediated Urinary Tract Infections

Immunogenic Potential and Therapeutic Efficacy of Multi‐Epitope Encapsulated Silk Fibroin Nanoparticles against Pseudomonas aeruginosa‐Mediated Urinary Tract Infections

Baicalein Inhibits the Staphylococcus aureus Biofilm and the LuxS/AI-2 System in vitro

Protein‐Mediated ZnO Synthesis for Self‐Healing in Natural Rubber

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