Characterization of the selectivity of a phenytoin imprinted polymer

Pap, Tímea; Horvai, George

doi:10.1016/j.chroma.2004.01.064

Cited by 33 publications

(21 citation statements)

References 20 publications

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“…This is achieved by arranging functional monomers around a template compound and then fixing the monomers in this spatial arrangement with a crosslinker [1]. An ideal molecularly imprinted polymer (MIP) has homogeneous binding sites which show no co-operative properties and no matrix effects [2]. Such an MIP could find uses as the sorbent in SPE [3][4][5][6], as the stationary phase in HPLC [7,8], and as the receptor layer in biosensors [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Mohajeri¹,

Ebrahimi²

2008

J of Separation Science

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A molecularly imprinted polymer (MIP) against lamotrigine (LTG) was prepared, characterized, and its recognition properties were compared with a blank nonimprinted polymer (NIP). Two classes of binding sites were found in the MIP--high affinity (K(D) = 16.2 microM) and low affinity (K(D) = 161.3 microM). Selectivity of the synthesized MIP was examined using compounds with similar structures or therapeutic uses to LTG. In compounds which had structural similarity to LTG, the presence of amine groups appeared to affect binding to the MIP, however overall shape of the molecule was also important. Under the optimal conditions developed, other anticonvulsant drugs tested did not bind the MIP. A molecularly imprinted SPE (MISPE) procedure was developed which had a recovery of 84-89%, interday variation of less than 3.4% and intraday variation of less than 2.8%. The MISPE procedure was compared with a routine liquid-liquid extraction (LLE) procedure used for the HPLC determination of LTG in serum from patients. The data indicated that the MIP synthesized showed both good selectivity and high affinity for LTG and could be used for the extraction of the drug from serum samples or as the receptor layer for an LTG selective biosensor.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Mohajeri¹,

Ebrahimi²

2008

J of Separation Science

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“…Isotherms may be studied and compared at different temperatures or with different adsorptives. If more than one compound is simultaneously adsorbed the isotherm is often called competitive isotherm, although simultaneous adsorption may also be synergistic [4].…”

Section: Introductionmentioning

confidence: 99%

Relationship between Commonly Used Adsorption Isotherm Equations Impedes Isotherm Selection

Dorkó

Szakolczai

Tóth

et al. 2017

Period. Polytech. Chem. Eng.

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“…More recently, they reported the characterization of the selectivity of a phenytoin-imprinted polymer [40]. This paper shows the theoretical and practical difficulties, which have to be considered and solved when real samples need to be analyzed in a wide range of analyte and interferant concentrations.…”

Section: Chromatographic and Batchwise Guest-binding Methodsmentioning

confidence: 98%

An insight into molecularly imprinted polymers for capillary electrochromatography

Liu

Lin

2004

Electrophoresis

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Molecularly imprinted polymers (MIPs) are actively being developed as a practical tool for affinity chromatographic supports. From the viewpoint of separation science, capillary electrochromatography (CEC) might be one of the more promising chromatographic techniques to be used in combination with the MIPs. However, up to the present, very little MIP work has involved CEC. This review gives a full overview of MIP including current trends in MIP, methods for the characterization of MIP, and methods for the preparation of MIP with particular emphasis on application of the resulting materials in CEC. To prepare MIPs with selectivity predetermined for a particular substance or group of structural analogues is an important factor for the development of a new format of CEC. From the fundamental research with the batch method, a better knowledge of imprint formation and imprint recognition will be helpful for expanding the application area of the combination of MIPs with CEC.

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Characterization of the selectivity of a phenytoin imprinted polymer

Cited by 33 publications

References 20 publications

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Preparation and characterization of a lamotrigine imprinted polymer and its application for drug assay in human serum

Relationship between Commonly Used Adsorption Isotherm Equations Impedes Isotherm Selection

An insight into molecularly imprinted polymers for capillary electrochromatography

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