Characterization of the seed proteins of African locust bean (Parkia biglobosa), African breadfruit (Treculia africana) and soybean (Glycine max)

Akin-Idowu, Pamela E.; Egbekunle, K.O.; Akinyemi, S. O. S.; Orkpeh, Uterdzua

doi:10.17660/actahortic.2018.1225.59

Cited by 1 publication

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mealy pulp from the species' fruits is a major source of energy and nutrients including carbohydrates, proteins, lipids, carotenoids, vitamins A, B, C, and oligoelements (Nyadanu et al, 2017). The seeds are an important source of plant protein and essential amino acids among rural communities who often have limited access to animal proteins due to high cost (Akin-Idowu et al, 2018;Alabi et al, 2005) and are also rich in energy value, saccharose, vitamin C, lipids, carbohydrates (Orwa et al, 2010) and bioactive components such as phenolic compounds which may contribute in health promoting properties (Dedehou et al, 2016;Okoye et al, 2014). P. biglobosa seeds are fermented to make a food condiment called Dawadawa (Iru) which is rich in dietary protein and serves as an important substitute for animal protein (Lamien et al, 2011;Nyadanu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Biodiversity assessment of African locust bean (Parkia biglobosa) accessions from Savanna and Forest zones of Nigeria as revealed by seed storage proteins and RAPD markers

Akin-Idowu¹,

Aduloju²,

Akinyoola³

et al. 2021

GenResJ

View full text Add to dashboard Cite

Understanding the level and distribution of genetic diversity in African locust bean (Parkia biglobosa) would strengthen breeding and conservation programmes towards domestication and sustainable use of this species. Sixteen accessions of P. globosa were assessed for variability based on seed morphology, seed protein and DNA profiling. Significant variation in seed characteristics were observed across locations. Seed protein profiling by SDS-PAGE revealed homogeneity as most bands were found common in all accessions, indicating that the protein profiles are highly conserved. Protein profiling separated the 16 accessions into four major clusters at 0.93 similarity coefficient. Most accessions grouping into Cluster 1 had a similarity coefficient of close to 100% and were from the Derived Savanna suggesting the presence of duplicates. Accessions NH/2016/P14, NH/2016/P03 and NH/2016/P04 grouped into clusters II, III and IV; respectively. Sixteen RAPD markers generated a total of 256 bands of which 63.67% were polymorphic. Gene diversity ranged from 0.41 to 0.93 and Polymorphic Information Content (PIC) from 0.39 to 0.93. The RAPD-based dendrogram separated accessions into six groups at 0.68 similarity coefficient. Based on a polymorphic seed storage protein marker a genetically distinct accession NH/2016/P04 could be exploited for breeding purposes. The homogeneity of alleles and narrow genetic base as revealed by RAPD and SDS-PAGE analyses suggests possible loss of intraspecific genetic diversity. Thus, intensification of germplasm collections across the different agroecological zones and characterization using specific markers will give a better understanding of diversity of P. biglobosa in order to enhance selection towards conservation, breeding and sustainable utilization.

show abstract

Section: Introductionmentioning

confidence: 99%