Characterization of the second long polar (LP) fimbriae of<i>Escherichia coli</i>O157:H7 and distribution of LP fimbriae in other pathogenic<i>E. coli</i>strains

Torres, Alfredo G.; Kanack, Kristen J.; Tutt, Christopher B.; Popov, Vsevolod L.; Kaper, James B.

doi:10.1111/j.1574-6968.2004.tb09774.x

Cited by 11 publications

(1 citation statement)

References 30 publications

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“…Four genetic variants of lpfA, namely lpfA (O157/OI-141), lpfA (O157/OI-154), lpfA (O26), and lpfA (O113), have been identified in Shiga toxin-producing E. coli (STEC) [ 74 ]. LpfA is associated with the adherence and invasion capacity of bacteria to epithelial cells [ 75 ], and its expression in E. coli strains is important for pathogenicity [ 35 , 76 ]. In our study, the gene for Long Polar Fimbriae Type I (lpfA), a recognized marker for virulent isolates of pathogenic E. coli , is more readily amplified by PCR in E. coli O91:H21 compared to the O22:H8 strain, although whole genome sequencing results revealed that both E. coli O91:H21 and E. coli O22:H8 have the same number of base pairs (in PPM) for the lpfA gene.…”

Section: Discussionmentioning

confidence: 99%

The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Chen,

McCulloch,

Das Neves

et al. 2023

Gut Pathog

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Background Formyl peptide receptor 2 (Fpr2) plays a crucial role in colon homeostasis and microbiota balance. Commensal E. coli is known to promote the regeneration of damaged colon epithelial cells. The aim of the study was to investigate the connection between E. coli and Fpr2 in the recovery of colon epithelial cells. Results The deficiency of Fpr2 was associated with impaired integrity of the colon mucosa and an imbalance of microbiota, characterized by the enrichment of Proteobacteria in the colon. Two serotypes of E. coli, O22:H8 and O91:H21, were identified in the mouse colon through complete genome sequencing. E. coli O22:H8 was found to be prevalent in the gut of mice and exhibited lower virulence compared to O91:H21. Germ-free (GF) mice that were pre-orally inoculated with E. coli O22:H8 showed reduced susceptibility to chemically induced colitis, increased proliferation of epithelial cells, and improved mouse survival. Following infection with E. coli O22:H8, the expression of Fpr2 in colon epithelial cells was upregulated, and the products derived from E. coli O22:H8 induced migration and proliferation of colon epithelial cells through Fpr2. Fpr2 deficiency increased susceptibility to chemically induced colitis, delayed the repair of damaged colon epithelial cells, and heightened inflammatory responses. Additionally, the population of E. coli was observed to increase in the colons of Fpr2−/− mice with colitis. Conclusion Commensal E. coli O22:H8 stimulated the upregulation of Fpr2 expression in colon epithelial cells, and the products from E. coli induced migration and proliferation of colon epithelial cells through Fpr2. Fpr2 deficiency led to an increased E. coli population in the colon and delayed recovery of damaged colon epithelial cells in mice with colitis. Therefore, Fpr2 is essential for the effects of commensal E. coli on colon epithelial cell recovery.

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Section: Discussionmentioning

confidence: 99%

The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Chen,

McCulloch,

Das Neves

et al. 2023

Gut Pathog

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Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174

Zhang

Nadirk

Kossow

et al. 2013

Environmental Microbiology

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Shiga toxin (Stx)-producing Escherichia coli (STEC) of serogroup O174 are human pathogenic intimin gene (eae)-negative STEC. To facilitate diagnosis and subtyping, we genotypically and phenotypically characterized 25 STEC O174 isolates from humans with different clinical outcomes and from animals and the environment. fliC genotyping resulted in four different genotypes (fliCH2 : n = 5; fliCH8 : n = 8; fliCH21 : n = 11; fliCH46 : n = 1). Twenty-three strains were motile expressing the corresponding H antigen; two non-motile isolates possessed fliCH8 . The stx genotypes and non-stx virulence loci, including toxins, serine-proteases and adhesins correlated well with serotypes but showed no differences with respect to the isolates' origins. Multilocus sequence typing identified seven sequence types that correlated with serotypes. Core gene typing further specified the four serotypes, including a previously unknown O174:H46 combination, and revealed distant relationships of the different serotypes within serogroup O174 and in relation to other haemolytic uremic syndrome (HUS)-associated STEC. Only serotype O174:H21 was associated with HUS. Differences in virulence factors and in the adherence capacity of STEC O174 corroborated this separation into four distinct groups. Our study provides a basis for O174 subtyping, unravels considerable genotypic and phenotypic heterogeneity and sheds light to potential environmental and animal reservoirs.

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Expression fusion immunogen by live attenuated Escherichia coli against enterotoxins infection in mice

Feng

Guan

2019

Microbial Biotechnology

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Summary Previous epidemiological studies have shown that enterotoxins from enterotoxigenic Escherichia coli (ETEC) appear to be the most important causes of neonatal piglet and porcine post‐weaning diarrhoea (PWD). Thus, it is necessary to develop an effective vaccine against ETEC infection. In the present study, the Kil cassette was inserted into the pseudogene yaiT by homologous recombination to create an attenuated E. coli double selection platform O142(yaiT‐Kil). After that, PRPL‐Kil was replaced with a fusion gene (LTA1‐STa13‐STb‐LTA2‐LTB‐STa13‐STb) to establish oral vaccines O142(yaiT::LTA1‐STa13‐STb‐LTA2‐LTB‐STa13‐STb) (ER‐T). Subsequently, BALB/c mice were orally immunized with ER‐T. Results showed that serum IgG and faecal sIgA responded against all ETEC enterotoxins and induced F41 antibody in BALB/c mice by orogastrically inoculation with recombinant E. coli ER‐T. Moreover, the determination of cellular immune response demonstrated that the stimulation index (SI) was significantly higher in immunized mice than in control mice, and a clear trend in the helper T‐cell (Th) response was Th2‐cell (IL‐4) exceed Th1‐cell (IFN‐γ).Our results indicated that recombinant E. coli ER‐T provides effective protection against ETEC infection.

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Characterization of the second long polar (LP) fimbriae ofEscherichia coliO157:H7 and distribution of LP fimbriae in other pathogenicE. colistrains

Cited by 11 publications

References 30 publications

The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174

Expression fusion immunogen by live attenuated Escherichia coli against enterotoxins infection in mice

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