The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Chen, Keqiang; McCulloch, John; Das Neves, Rodrigo; Rodrigues, Gisele; Hsieh, Wang-Ting; Gong, Wanghua; Yoshimura, Teizo; Huang, Jiaqiang; O’hUigin, Colm; Difilippantonio, Simone; McCollum, Matthew; Jones, Georgette; Durum, Scott K.; Trinchieri, Giorgio; Wang, Ji Ming

doi:10.1186/s13099-023-00557-w

Cited by 3 publications

(3 citation statements)

References 79 publications

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“…Fpr2 deficiency increased susceptibility to chemically induced colitis, delayed the repair of damaged colon epithelial cells, and heightened inflammatory responses. Additionally, the population of Escherichia coli was observed to increase in the colon of Fpr2 −/− mice with colitis (Chen et al 2023). These results indicate that FPR2 is critical in mediating homeostasis, inflammation, and epithelial repair processes in the colon.…”

Section: Discussionmentioning

confidence: 99%

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Probiotic Lactobacilli activate Formyl-Peptide Receptor 2

Dorothee,

Ralf,

Ahmed

et al. 2024

Preprint

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Changes in the composition of the human microbiota can negatively impact human health. Probiotic bacteria like many lactobacilli help prevent or repair dysbiosis but it is largely unclear which molecules of these bacteria mediate the probiotic effects. Given the extensive crosstalk between the immune system and microbiome members, we investigated whether lactobacilli activate the formyl-peptide receptor 2 (FPR2), a pattern recognition receptor that is expressed on the surface of intestinal epithelial cells and known to promote wound healing and immune homeostasis. Probiotic strains of Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, and Lacticaseibacillus rhamnosus were isolated from probiotic compounds and sequenced. Calcium influx experiments in FPR1 or FPR2 overexpressing HL60 cells, and primary human neutrophils, along with pharmacological inhibition of FPR2, revealed that culture filtrates of the isolated lactobacilli strongly activate FPR2, promote killing of the methicillin resistant S. aureus USA300 and induce neutrophil chemotaxis. Pretreatment of culture filtrates with proteinase K reduced FPR2 activity, indicating that the FPR2 ligands are peptides. In silico analysis of the amphipathic properties of the signal peptides of lactic acid bacteria identified selected signal peptides of L. plantarum with the ability to predominantly activate FPR2 in vitro. Thereby, via targeted activation of FPR2, peptides released by some lactobacilli are likely to positively influence the outcome of inflammatory gut diseases and could be used to treat inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

“…Gut epithelia cells possess a variety of receptors to recognize bacterial molecules such as peptidoglycan, lipopeptides, or formylated peptides (Burgueno and Abreu 2020; Chen et al 2023; Alam et al 2014). Among those receptors, FPR2 has a special role.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Lactobacilli activate Formyl-Peptide Receptor 2

Dorothee,

Ralf,

Ahmed

et al. 2024

Preprint

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“…In this article [ 1 ] the author name Gisele Rodrigues was incorrectly written as Gisele Roderigues.…”

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confidence: 99%

Correction: The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Chen,

McCulloch,

Das Neves

et al. 2023

Gut Pathog

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Effects of Tumor-Associated E. coli Metabolites on Migration of Colorectal Cancer Cells

Ignatova,

Pryazhnikova,

Seliverstov

et al. 2024

Preprint

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Colorectal tumors have a close connection with the gut microbiome. Correlation between rearrangement in microbiome composition and disease progression has already been shown. However, the questions about the mechanisms underlying microorganisms and cancer cells interaction and the immediate effects of tumor-associated microbiomes on cancer cells remain open. In this work, we investigated the effects of metabolites of tumor-associated E.coli strains on the growth and migration of human colorectal cancer cell lines (HCT116, SW480 and HT29). Differences in the spectrum of synthesized organic acids from tumor-associated and probiotic M-17 strains were revealed. Specifically, tumor-associated E.coli produced more fumaric, malic and maleic acids, whereas the M-17 - more propionic, 2-oxobutyric and α-ketoglutaric acids. Upon exposure to metabolites from tumor-associated E.coli strains, HCT116 and SW480 cells showed an increased migration activity and HT29 cells - decreased migration activity in 2D and 3D culture models. Immunocytochemistry assay revealed decrease of E-cadherin in HCT116 and SW480 cells and FAK- in HT29, which explain different effects of E.coli metabolites on migratory capacity of colorectal cancer cells. Therefore, these results suggest that the effect of tumor-associated E.coli strains on cancer cells migration depends on their innate type of migration - single-cell or collective migration.

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The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Cited by 3 publications

References 79 publications

Probiotic Lactobacilli activate Formyl-Peptide Receptor 2

Probiotic Lactobacilli activate Formyl-Peptide Receptor 2

Correction: The beneficial effects of commensal E. coli for colon epithelial cell recovery are related with Formyl peptide receptor 2 (Fpr2) in epithelial cells

Effects of Tumor-Associated E. coli Metabolites on Migration of Colorectal Cancer Cells

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