Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis.

Shahin, Roberta D.; Brennan, Michael J.; Li, Z M; Meade, Bruce D.; Manclark, C R

doi:10.1084/jem.171.1.63

Cited by 139 publications

(68 citation statements)

References 21 publications

(15 reference statements)

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“…Surprisingly, these vaccinated piglets had levels of specific IgA and IgG antibodies in their serum prior to challenge comparable to those of the passively transferred antibodies in piglets born to vaccinated sows. Although our results are in contrast to other studies demonstrating a protective role for passively transferred pertussis-specific antibodies (73,74), we cannot exclude the role of these antibodies and believe that the humoral immune response is involved in several aspects of protection against pertussis. However, our data demonstrate that protection against pertussis depends on a combination of antibody and cellular immune response, as has been reported elsewhere (75).…”

Section: Maternal Cytokines and Immunity To Pertussiscontrasting

confidence: 55%

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

et al. 2017

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Maternal vaccination represents a potential strategy to protect both the mother and the offspring against life-threatening infections. This protective role has mainly been associated with antibodies, but the role of cell-mediated immunity, in particular passively transferred cytokines, is not well understood. Here, using a pertussis model, we have demonstrated that immunization of pregnant sows with heat-inactivated bacteria leads to induction of a wide range of cytokines (e.g., tumor necrosis factor alpha [TNF-␣], gamma interferon [IFN-␥], interleukin-6 [IL-6], IL-8, and IL-12/IL-23p40) in addition to pertussis-specific antibodies. These cytokines can be detected in the sera and colostrum/milk of vaccinated sows and subsequently were detected at significant levels in the serum and bronchoalveolar lavage fluid of piglets born to vaccinated sows together with pertussis-specific antibodies. In contrast, active vaccination of newborn piglets with heat-inactivated bacteria induced high levels of specific IgG and IgA but no cytokines. Although the levels of antibodies in vaccinated piglets were comparable to those of passively transferred antibodies, no protection against Bordetella pertussis infection was observed. Thus, our results demonstrate that a combination of passively transferred cytokines and antibodies is crucial for disease protection. The presence of passively transferred cytokines/antibodies influences the cytokine secretion ability of splenocytes in the neonate, which provides novel evidence that maternal immunization can influence the newborn's cytokine milieu and may impact immune cell differentiation (e.g., Th1/Th2 phenotype). Therefore, these maternally derived cytokines may play an essential role both as mediators of early defense against infections and possibly as modulators of the immune repertoire of the offspring.

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Section: Maternal Cytokines and Immunity To Pertussiscontrasting

confidence: 55%

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

et al. 2017

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“…Studies in animals and humans have indicated that pertactin plays an important role in immunity to pertussis Cherry et al, 1998 ;Shahin et al, 1990 ;Storsaeter et al, 1998). Especially relevant are the results of two independent field trials, which revealed a correlation between pertactin antibodies and clinical protection (Cherry & Olin 1999 ;Cherry et al, 1998 ;Storsaeter et al, 1998).…”

Section: Discussionmentioning

confidence: 95%

“…A number of studies have shown that the B. pertussis fimbriae, filamentous haemagglutinin, pertussis toxin and pertactin can induce protection in both animals and humans (Sato & Sato, 1984 ;Zhang et al, 1985 ;Kimura et al, 1990 ;Robinson et al, 1985 ;Shahin et al, 1990). Therefore, these proteins have been included in acellular pertussis vaccines.…”

Section: Introductionmentioning

confidence: 99%

Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity

et al. 2001

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In several countries pertussis is re-emerging, despite a high vaccination coverage. It is suggested that antigenic divergence between Bordetella pertussis vaccine strains and circulating strains, in particular with respect to pertactin, has contributed to pertussis re-emergence. Polymorphism in pertactin is essentially limited to region 1, which is composed of repeats and is located adjacent to an Arg-Gly-Asp motif implicated in adherence. Evidence is provided for the immunological relevance of polymorphism in region 1. Region 1 was found to contain a B-cell epitope recognized in both humans and mice. Furthermore, variation in region 1 affected antibody binding and, in a mouse respiratory infection model, the efficacy of a whole-cell vaccine. Moreover, passive and active immunization indicated that region 1 confers protective immunity. An mAb directed against a linear conserved epitope conferred crossimmunity against isolates with distinct pertactin variants. The results indicate an important role of region 1 of pertactin in immunity.

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“…cough, produces many virulence factors (Leininger et al, 1990(Leininger et al, , 1991Relman et a[., 1989;Weiss & animal 1986). A'nurnber of these facto filamentous haemagglutinin, fimbriae, are proteins involved rs, p&tussis toxin (PT); models showed that these antigens are efficacious in pertactin (prn) and preventing B. pertussis infections, thus supporting their in intoxication and/or use in the new generation of acellular pertussis vaccines (Cahill et al, 1993;Edwards, 1993;Kimura & KunoSakai, 1990;Peppoloni et al, 1995;Shahin et al, 1990).…”

Section: Introduction Adhesion Processes Antigens Responsiblementioning

confidence: 99%

Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children

et al. 1999

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Bordetella pertussis shows polymorphism in two proteins, pertactin (Prn) and the pertussis toxin (PT) S 1 subunit, which are important for immunity. A previous study has shown antigenic shifts in these proteins in the Dutch B. pertussis population, and it was suggested that these shifts were driven by vaccination. The recent Italian clinical trial provided the opportunity to compare the frequencies of Prn and PT 51 subunit variants in strains isolated from unvaccinated children, and from children vaccinated with two acellular and one whole-cell pertussis vaccine. Four Prn variants (Prnl, Prn2, Prn3 and Prn5) were found in the 129 strains analysed. Prnl, Prn2 and Prn3 have been described previously, whereas Prn5 is a novel variant. Prnl, Prn2, Prn3 and Prn5 were found in, respectively, 6,41, 51 and 2 % of the strains. The B. pertussis strains used to produce the vaccines administered in the clinical trial were found to produce Prnl, or a type which differed from Prnl in one amino acid. The frequency of the Prnl variant was found to be lowest in the strains isolated from vaccinated groups, suggesting that Prnl strains are more affected by vaccine-induced immunity than Prn2 and Prn3 strains. Only one PT 51 type (SlA) was observed in the examined strains, which was distinct from the types produced by the vaccine strains (SlB and SlD). The S1A type also predominates in the Dutch B. pertussis population. The genetic relationship among B. pertussis strains analysed by ISl002-based DNA fingerprinting revealed that three fingerprint types predominate, representing more than 70% of the strains. Prn2 strains showed a greater variety of fingerprint types compared to Prn3, suggesting that Prn3 has emerged more recently. The results are discussed in the light of vaccine-driven evolution.

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Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis.

Cited by 139 publications

References 21 publications

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets

Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity

Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children

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