Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity

King, Audrey J.; Berbers, Guy A. M.; Oirschot, Hans F. L. M. van; Hoogerhout, P.; Knipping, Karen; Mooi, Frits R.

doi:10.1099/00221287-147-11-2885

Cited by 101 publications

(113 citation statements)

References 39 publications

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“…This T1 loop comprises a proline-rich repeat, GGXXP 5 , designated "region 1," which is polymorphic in different clinical isolates and vaccine strains (336). Antibodies to this region have been found in patients with pertussis and are associated (246). The passenger domain of pertactin is the largest ␤-helix described to date, having 16 rungs or coils.…”

Section: Pertactinmentioning

confidence: 99%

Type V Protein Secretion Pathway: the Autotransporter Story

Henderson

Navarro-Garcı́a

Desvaux

et al. 2004

Microbiol Mol Biol Rev

713

795

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Gram-negative bacteria possess an outer membrane layer which constrains uptake and secretion of solutes and polypeptides. To overcome this barrier, bacteria have developed several systems for protein secretion. The type V secretion pathway encompasses the autotransporter proteins, the two-partner secretion system, and the recently described type Vc or AT-2 family of proteins. Since its discovery in the late 1980s, this family of secreted proteins has expanded continuously, due largely to the advent of the genomic age, to become the largest group of secreted proteins in gram-negative bacteria. Several of these proteins play essential roles in the pathogenesis of bacterial infections and have been characterized in detail, demonstrating a diverse array of function including the ability to condense host cell actin and to modulate apoptosis. However, most of the autotransporter proteins remain to be characterized. In light of new discoveries and controversies in this research field, this review considers the autotransporter secretion process in the context of the more general field of bacterial protein translocation and exoprotein function

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Section: Pertactinmentioning

confidence: 99%

Type V Protein Secretion Pathway: the Autotransporter Story

Henderson

Navarro-Garcı́a

Desvaux

et al. 2004

Microbiol Mol Biol Rev

713

795

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“…An important question is whether the observed mismatches affect the efficacy of pertussis vaccines. Using a mouse model, we have found that variation in Prn affects the efficacy of a WCV (21). One may argue that, since ACVs induce higher titers against Ptx and Prn compared to WCV, they may be less affected by the mismatch.…”

mentioning

confidence: 99%

Multilocus Sequence Typing of Bordetella pertussis Based on Surface Protein Genes

Loo

Heuvelman²,

King³

et al. 2002

J Clin Microbiol

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150

137

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Despite more than 50 years of vaccination, Bordetella pertussis has remained endemic in The Netherlands, causing epidemic outbreaks every 3 to 5 years. Strain variation may play a role in the persistence of B. pertussis and was studied by sequencing 15 genes coding for surface proteins, including genes for all five components of acellular pertussis vaccines: pertussis toxin (Ptx), pertactin (Prn), filamentous hemagglutinin, and fimbriae (Fim2 and Fim3). A low level of allelic variation was observed, confirming a recent evolutionary origin of B. pertussis. In modern isolates, polymorphism was observed only in prn, ptxS1, ptxS3, and tcfA. Polymorphism in ptxS1, ptxS3, and tcfA was used to categorize isolates in multilocus sequence types (MLSTs). Analysis of Dutch isolates from 1949 to 1999 revealed five MLSTs, which showed a highly dynamic temporal behavior. We observed significant changes in the MLSTs after the introduction of pertussis vaccination in The Netherlands. Epidemic years were found to be associated with the expansion of MLST-4 or MLST-5. MLST-5 showed a remarkable expansion from 10% in 1997 to 80% in 1999. The MLST analysis was extended to a number of widely separated geographic regions: Finland, Italy, Japan, and the United States. MLST-4 and MLST-5 were found to dominate in Italy and the United States. In Finland and Japan, MLST-3 and MLST-2, respectively, were predominant. Thus, although each region showed distinctive MLST frequencies, in three of the five regions MLST-4 and MLST-5 were predominant. These types may represent newly emerged, successful clones. The identification of highly successful clones may shed light on the question of how B. pertussis is able to maintain itself in vaccinated populations.

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“…However, their efficacy against subclinical infection is doubtful, as the majority of vaccinated populations test positive for subsequent infection (10,17), suggesting that the bacterium successfully infects immune and/or vaccinated individuals. Using animal models, we and others have previously shown that although B cells are necessary for B. pertussis clearance from the respiratory tract (18)(19)(20), adoptively transferred serum antibodies have little or no effect on bacterial numbers for the first 7 days after inoculation (18)(19)(20)(21) but begin to control and clear the bacteria thereafter. The ability to resist rapid antibody-mediated clearance may increase the duration and intensity of infection, both of which facilitate the transmission of the bacteria and would therefore be critical to the endemism of B. pertussis in vaccinated populations.…”

Section: Introductionmentioning

confidence: 99%

Pertussis toxin inhibits neutrophil recruitment to delay antibody-mediated clearance of Bordetella pertussis

Kirimanjeswara¹

2005

Journal of Clinical Investigation

134

127

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Whooping cough is considered a childhood disease, although there is growing evidence that children are infected by adult carriers. Additionally, increasing numbers of vaccinated adults are being diagnosed with Bordetella pertussis disease. Thus it is critical to understand how B. pertussis remains endemic even in highly vaccinated or immune populations. Here we used the mouse model to examine the nature of sterilizing immunity to B. pertussis. Antibodies were necessary to control infection but did not rapidly clear B. pertussis from the lungs. However, antibodies affected B. pertussis after a delay of at least a week by a mechanism that involved neutrophils and Fc receptors, suggesting that neutrophils phagocytose and clear antibody-opsonized bacteria via Fc receptors. B. pertussis blocked migration of neutrophils and inhibited their recruitment to the lungs during the first week of infection by a pertussis toxin-dependent (PTx-dependent) mechanism; a PTx mutant of B. pertussis induced rapid neutrophil recruitment and was rapidly cleared from the lungs by adoptively transferred antibodies. Depletion of neutrophils abrogated the defects of the PTx mutant. Together these results indicate that PTx inhibits neutrophil recruitment, which consequently allows B. pertussis to avoid rapid antibody-mediated clearance and therefore successfully infect immune hosts.

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Role of the polymorphic region 1 of the Bordetella pertussis protein pertactin in immunity

Cited by 101 publications

References 39 publications

Type V Protein Secretion Pathway: the Autotransporter Story

Type V Protein Secretion Pathway: the Autotransporter Story

Multilocus Sequence Typing of Bordetella pertussis Based on Surface Protein Genes

Pertussis toxin inhibits neutrophil recruitment to delay antibody-mediated clearance of Bordetella pertussis

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