Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

Miao, Jun; Sun, Jinsheng; Liu, Dongcheng; Li, Bin; Zhang, Aimin; Li, Zhensheng; Tong, Yiping

doi:10.1016/s1673-8527(08)60135-6

Cited by 50 publications

(36 citation statements)

References 40 publications

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“…Several genes that code for Pi-responsive transporters have been described in rice , barley (Hordeum vulgare; Schü nmann et al, 2004a), wheat (Triticum aestivum; Tittarelli et al, 2007;Miao et al, 2009), and maize (Nagy et al, 2006), and it is known that in maize and rice roots, the up-regulation of P transport-and P recycling-related genes was the most extensive adaptation under Pi starvation, as reflected by the up-regulation of several putative Pi transporters, phosphatase, and RNase genes (Wasaki et al, 2003;Calderon-Vazquez et al, 2008).…”

Section: Molecular Responses To P Starvation In Maizementioning

confidence: 99%

“…P1BS, an imperfect palindromic DNA sequence, was previously identified as the PHR1 target in promoters of P-regulated genes in Arabidopsis (Rubio et al, 2001). Furthermore, P1BS mediates P starvation expression in barley (Schü nmann et al, 2004b), whereas in wheat, the P1BS motif was found in the promoter of the gene TaPHT1.2_D1 (Tittarelli et al, 2007;Miao et al, 2009), a Pi transporter regulated by P starvation in roots, suggesting that PHR1-like elements could be regulating P starvation responses in cereals.…”

Section: Putative Regulators Of P Starvation Responses In Maizementioning

confidence: 99%

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Phosphate Deprivation in Maize: Genetics and Genomics

2011

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Section: Molecular Responses To P Starvation In Maizementioning

confidence: 99%

Section: Putative Regulators Of P Starvation Responses In Maizementioning

confidence: 99%

Phosphate Deprivation in Maize: Genetics and Genomics

2011

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“…However, the functions of HvPHT1;3 and HvPHT1;4 in Pi homeostasis are not clear. Only one wheat PHT1 gene, TaPHT1;2D1, has been characterized so far (Miao et al, 2009), although several TaPHT1 genes are reported (Davies et al, 2002;Miao et al, 2009). Whole genomic sequences of barley and wheat are not yet available, which has hindered progress toward the functional characterization of PHT1 genes and their regulation in barley and wheat.…”

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confidence: 99%

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

et al. 2011

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Genetic variation in phosphorus (P) efficiency exists among wheat (Triticum aestivum) and barley (Hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. High-and low-affinity phosphate (Pi) PHT1 transporters play an indispensable role in P acquisition and remobilization. However, little is known about genetic variation in PHT1 gene expression and association with P acquisition efficiency (PAE) and P utilization efficiency (PUE). Here, we present quantitative analyses of transcript levels of high-and low-affinity PHT1 Pi transporters in four barley genotypes differing in PAE. The results showed that there was no clear pattern in the expression of four paralogs of the high-affinity Pi transporter HvPHT1;1 among the four barley genotypes, but the expression of a low-affinity Pi transporter, HvPHT1;6, and its close homolog HvHPT1;3 was correlated with the genotypes differing in PUE. Interestingly, the expression of HvPHT1;6 and HvPHT1;3 was correlated with the expression of HvIPS1 (for P starvation inducible; noncoding RNA) but not with HvIPS2, suggesting that HvIPS1 plays a distinct role in the regulation of the low-affinity Pi transporters. In addition, high PUE was found to be associated with high root-shoot ratios in low-P conditions, indicating that high carbohydrate partitioning into roots occurs simultaneously with high PUE. However, high PUE accompanying high carbon partitioning into roots could result in low PAE. Therefore, the optimization of PUE through the modification of low-affinity Pi transporter expression may assist further improvement of PAE for low-input agriculture systems.

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“…Phosphate transporters, for instance TaPht1;2 (Miao et al 2009), seem to belong to the group of sugar-modulated genes under P-starvation Karthikeyan et al 2007;Hammond and White 2008).…”

Section: P Starvation Signalingmentioning

confidence: 99%

“…The Pht1 promoters contain the target elements for transcription factors of the P signalling network, for instance the P1BS cis-element as a target for PHR1 (Schünmann et al 2004;Ren et al 2012a) or the WRKY-binding W-box as a target for WRKY75 (Devaiah et al 2007a, b;Miao et al 2009), suggesting their embedding in the cross-talk of P signalling during P starvation to maintain P homeostasis.…”

mentioning

confidence: 99%

Efficient Mineral Nutrition: Genetic Improvement of Phosphate Uptake and Use Efficiency in Crops

Gruen

Broadley

Büchner

et al. 2014

Plant Ecophysiology

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Phosphorus (P) is an essential macronutrient for plants, the lack of which can be a major constraint for agricultural productivity. Economic, political and environmental factors have prioritized the need for research on P acquisition efficiency (PAE), P utilization efficiency (PUE) and P fertiliser uptake efficiency in crops. P has critical functions in plants and complex interactions in soils. Appropriate screening approaches and implications of improvement in crop production are discussed. P acquisition is mediated by members of phosphate transporter families and the roles of these phosphate transporters as well as enzymes involved in P partitioning and re-translocation are complex. There is also a critical importance of regulatory genes including transcription factors, signalling pathways and apparently other P-responsive genes with unknown function. Furthermore, morphological and biochemical responses enhance P solubility in the soil and facilitate uptake and include root plasticity, secretion processes and symbioses. Exploitation of genetic variation, classical breeding and biotechnological gene modification of target genes are future routes for crop improvement. There is a need for selection not just for uptake but also focussing on P storage pools within cells and tissues, and additionally a consideration of crop P requirements during the different growth stages of crops. The review concludes with a summary giving an outlook to future questions related to crop PAE/PUE improvement.

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Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

Cited by 50 publications

References 40 publications

Phosphate Deprivation in Maize: Genetics and Genomics

Phosphate Deprivation in Maize: Genetics and Genomics

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

Efficient Mineral Nutrition: Genetic Improvement of Phosphate Uptake and Use Efficiency in Crops

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