Characterization of the Populus Rab family genes and the function of PtRabE1b in salt tolerance

Zhang, Jin; Yu, Li; Liu, Bobin; Wang, Limin; Zhang, Li; Hu, Jianjun; Zheng, Huanquan; Lu, Mengzhu

doi:10.1186/s12870-018-1342-1

Cited by 30 publications

(29 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the Ka, the Ks, and the Ka/Ks ratios were estimated by DnaSP v5 [53]. Divergence time (T) was evaluated by T = Ks/(2 × 9.1 × 10 −9 ) × 10 −6 million years ago (Mya) [54].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors

Tian

Liu

Wang

et al. 2019

Plants

View full text Add to dashboard Cite

Jasmonate ZIM-domain (JAZ) family proteins are the key repressors in the jasmonate signaling pathway and play crucial roles in plant development, defenses, and responses to stresses. However, our knowledge about the JAZ protein family in petunia is limited. This research respectively identified 12 and 16 JAZ proteins in two Petunia progenitors, Petunia axillaris and Petunia inflata. Phylogenetic analysis showed that the 28 proteins could be divided into four groups (Groups A–D) and further classified into six subgroups (A1, A2, B1, B3, C, and D1); members in the same subgroup shared some similarities in motif composition and sequence structure. The Ka/Ks ratios of seven paralogous pairs were less than one, suggesting the petunia JAZ family might have principally undergone purifying selection. Quantitative real-time PCR (qRT-PCR) analysis revealed that PaJAZ genes presented differential expression patterns during the development of flower bud and anther in petunia, and the expression of PaJAZ5, 9, 12 genes was generally up-regulated after MeJA treatment. Subcellular localization assays demonstrated that proteins PaJAZ5, 9, 12 were localized in nucleus. Yeast two hybrid (Y2H) elucidated most PaJAZ proteins (PaJAZ1-7, 9, 12) might interact with transcription factor MYC2. This study provides insights for further investigation of functional analysis in petunia JAZ family proteins.

show abstract

“…Then, the Ka, the Ks, and the Ka/Ks ratios were estimated by DnaSP v5 [53]. Divergence time (T) was evaluated by T = Ks/(2 × 9.1 × 10 −9 ) × 10 −6 million years ago (Mya) [54].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors

Tian

Liu

Wang

et al. 2019

Plants

View full text Add to dashboard Cite

show abstract

“…However, in our previous study we determined that chickpea CaRabC genes were highly expressed in response to salinity and rapid dehydration but down-regulated by drought [36]. In poplar, the overexpression of a constitutively activated PtRabE1b enhanced growth in the presence of salt [37].…”

Section: Introductionmentioning

confidence: 93%

Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)

et al. 2020

View full text Add to dashboard Cite

Background Chickpea is an important legume and is moderately tolerant to salinity stress during the growing season. However, the level and mechanisms for salinity tolerance can vary among accessions and cultivars. A large family of CaRab-GTP genes, previously identified in chickpea, is homologous to intracellular vesicle trafficking superfamily genes that play essential roles in response to salinity stress in plants. Results To determine which of the gene family members are involved in the chickpea salt response, plants from six selected chickpea accessions (Genesis 836, Hattrick, ICC12726, Rupali, Slasher and Yubileiny) were exposed to salinity stress and expression profiles resolved for the major CaRab-GTP gene clades after 5, 9 and 15 days of salt exposure. Gene clade expression profiles (using degenerate primers targeting all members of each clade) were tested for their relationship to salinity tolerance measures, namely plant biomass and Na+ accumulation. Transcripts representing 11 out of the 13 CaRab clades could be detected by RT-PCR, but only six (CaRabA2, −B, −C, −D, −E and −H) could be quantified using qRT-PCR due to low expression levels or poor amplification efficiency of the degenerate primers for clades containing several gene members. Expression profiles of three gene clades, CaRabB, −D and −E, were very similar across all six chickpea accessions, showing a strongly coordinated network. Salt-induced enhancement of CaRabA2 expression at 15 days showed a very strong positive correlation (R2 = 0.905) with Na+ accumulation in leaves. However, salinity tolerance estimated as relative plant biomass production compared to controls, did not correlate with Na+ accumulation in leaves, nor with expression profiles of any of the investigated CaRab-GTP genes. Conclusion A coordinated network of CaRab-GTP genes, which are likely involved in intracellular trafficking, are important for the salinity stress response of chickpea plants.

show abstract

“…The GTPase RabE is located in the Golgi apparatus and the plasma membrane, where it plays an important role in vesicle transport [95]. The overexpression of constitutively active PtRabE1b conferred salt tolerance in poplar [96]. This gene is directly co-expressed with many genes involved in salt tolerance, such as HSFA4a , SOS2 , MPK19 , and the Ca 2+ signaling-related genes CAM7 , CKL6 , and calcium exchanger.…”

Section: Candidate Genes Used For the Genetic Improvement Of Salt mentioning

confidence: 99%

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Zhang

Liu

Chen

et al. 2019

IJMS

View full text Add to dashboard Cite

Salt stress (SS) has become an important factor limiting afforestation programs. Because of their salt tolerance and fully sequenced genomes, poplars (Populus spp.) are used as model species to study SS mechanisms in trees. Here, we review recent insights into the physiological and molecular responses of Populus to SS, including ion homeostasis and signaling pathways, such as the salt overly sensitive (SOS) and reactive oxygen species (ROS) pathways. We summarize the genes that can be targeted for the genetic improvement of salt tolerance and propose future research areas.

show abstract

Characterization of the Populus Rab family genes and the function of PtRabE1b in salt tolerance

Cited by 30 publications

References 51 publications

Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors

Genome-Wide Identification and Characterization of JAZ Protein Family in Two Petunia Progenitors

Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)

Progress in Understanding the Physiological and Molecular Responses of Populus to Salt Stress

Contact Info

Product

Resources

About