Characterization of the polysaccharides chemical diversity of the cyanobacteria Arthrospira platensis

Phélippé, Myriam; Gonçalves, Olivier; Thouand, Gérald; Cogne, Guillaume; Laroche, Céline

doi:10.1016/j.algal.2019.101426

Cited by 58 publications

(39 citation statements)

References 61 publications

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“…In general, the polysaccharides of cyanobacteria include storage polysaccharides (SPS) that are accumulated intracellularly, extracellular polysaccharides (EPS) or released polysaccharides (RPS) that release in the culture medium, and capsular polysaccharides (CPS) or bound polysaccharides (BPS) that are associated with cell wall [51–53]. Under nitrogen limitation condition, the main carbohydrate in A. platensis was SPS (formerly considered as glycogen), usually accounting for 40–65%DW [51]. The CPS or BPS, which were usually extracted by hot water, accounted for less than 8%DW under nitrogen limitation and their contents were negatively related to nitrogen supply [54].…”

Section: Resultsmentioning

confidence: 99%

Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Liu

Yao

Sun

et al. 2019

Biotechnol Biofuels

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Background Carbohydrates are major biomass source in fuel-targeted biorefinery. Arthrospira platensis is the largest commercialized microalgae with good environmental tolerance and high biomass production. However, the traditional target of A. platensis cultivation is the protein, which is the downstream product of carbohydrates. Aiming to provide the alternative non-food carbohydrates source, the feasible manipulation technology on the cultivation is needed, as well as new separation methodology to achieve maximum utilization of overall biomass. Results The present study aimed to demonstrate the feasibility of industrially producing carbohydrate-enriched A. platensis and characterize the structure of the polysaccharide involved. Cultivated in industrial-scale outdoor open raceway ponds under nitrogen limitation, A. platensis accumulated maximally 64.3%DW of carbohydrate. The maximum biomass and carbohydrate productivity reached 27.5 g m −2 day −1 and 26.2 g m −2 day −1 , respectively. The efficient extraction and purification of the polysaccharides include a high-pressure homogenization-assisted hot water extraction followed by flocculation with a non-toxic flocculant ZTC1 + 1, with the polysaccharide purity and total recovery reaching 81% and 75%, respectively. The purified polysaccharide was mainly composed of (1→3)(1→4)- or (1→3)(1→2)-α-glucan with a molecular weight of 300–700 kDa, which differed from the commonly acknowledged glycogen. Conclusions By the way of controlled nitrogen limitation, the high carbohydrate production of A. platensis in the industrial scale was achieved. The α-glucan from A. platensis could be a potential glucose source for industrial applications. A non-toxic separation method of carbohydrate was applied to maintain the possibility of utilization of residue in high-value field. Electronic supplementary material The online version of this article (10.1186/s13068-019-1470-3) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Liu

Yao

Sun

et al. 2019

Biotechnol Biofuels

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“…The ratio EPS/glycogen was found higher with lower light intensities. In addition, EPS monosaccharides composition was significantly different among different light intensities applied [86].…”

Section: Process Conditionsmentioning

confidence: 92%

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

et al. 2020

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Cyanobacteria have the potential to become an industrially sustainable source of functional biopolymers. Their exopolysaccharides (EPS) harbor chemical complexity, which predicts bioactive potential. Although some are reported to excrete conspicuous amounts of polysaccharides, others are still to be discovered. The production of this strain-specific trait can promote carbon neutrality while its intrinsic location can potentially reduce downstream processing costs. To develop an EPS cyanobacterial bioprocess (Cyano-EPS) three steps were explored: the selection of the cyanobacterial host; optimization of production parameters; downstream processing. Studying the production parameters allow us to understand and optimize their response in terms of growth and EPS production though many times it was found divergent. Although the extraction of EPS can be achieved with a certain degree of simplicity, the purification and isolation steps demand experience. In this review, we gathered relevant research on EPS with a focus on bioprocess development. Challenges and strategies to overcome possible drawbacks are highlighted.

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“…However, the first one was cultured outdoor, while the latter was cultured indoor. Moreover, the meta-bibliographic data analysis and experiments conducted by Phelippe, et al [42] has shown that total carbohydrate content is increased at high temperature with a mean of 41% DW (dry weight) for cultures conducted at more than 36 • C, and only 27% DW for lower temperatures. Same trend has been observed for high light intensities, with additionally a modulation between glycogen and EPS repartition and a modification in EPS composition.…”

Section: Characterization Of Eps Of Cyanobacteria Strainsmentioning

confidence: 98%

“…LEB 18 were cultivated in Zarrouk medium [39] and C. fusca in BG 11 medium [40]. These microalgae were selected, since there are previous studies that show the influence of SMF on growth, but the polysaccharides were not evaluated before [16,36,41], besides Spirulina and Chlorella has been widely studied due to their high polysaccharides content [1,7,42].…”

Section: Microalgae and Culture Conditionsmentioning

confidence: 99%

Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains

et al. 2021

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Microalgae are able to produce many valuable biomolecules, such as polysaccharides, that presents a large diversity of biochemical structures and functions as antioxidant, antifungal, anticancer, among others. Static magnetic fields (SMF) influence the metabolism of microorganisms and has been shown as an alternative to increase microalgae biomass, yield and compounds production. Especially, some studies have highlighted that SMF application could enhance carbohydrate content. This study aimed to evaluate different conditions of SMF on Spirulina and Chlorella in indoor and outdoor conditions, in order to confirm the influence of SMF on polysaccharides production, evaluating which polysaccharidic fraction could be enhanced by SMF and highlighting a possible modification in EPS composition. Starch from Chlorella and exopolysaccharides (EPS) from Spirulina were quantified and characterized. SMF increased the starch content in Chorella fusca biomass. EPS productions from A. platensis and Spirulina sp. were not significantly increased, and global composition appeared similar to the controls (constituted basically of 80%–86% neutral sugars and 13%–19% uronic acids). However, the monosaccharide composition analysis revealed a significant modification of composition, i.e., the amount of fucose, arabinose, rhamnose, galactose and glucuronic acid was increased, while the glucose content was decreased. SMF application led to significant modification of polysaccharides production and this study demonstrate that combining the outdoor conditions with SMF, the starch content and EPS composition was positively affected.

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Characterization of the polysaccharides chemical diversity of the cyanobacteria Arthrospira platensis

Cited by 58 publications

References 61 publications

Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Exopolysaccharides from Cyanobacteria: Strategies for Bioprocess Development

Static Magnetic Fields Effects on Polysaccharides Production by Different Microalgae Strains

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