Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Liu, Qishun; Yao, Changhong; Sun, Yongxin; Chen, Wei; Tan, Haidong; Cao, Xupeng; Xue, Song; Yin, Heng

doi:10.1186/s13068-019-1470-3

Cited by 40 publications

(29 citation statements)

References 52 publications

(75 reference statements)

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“…In all the studies [ 36 , 37 , 38 , 39 , 40 , 41 ], stirring during the cultivation of microalgae was a factor that reduces their ability to produce carbohydrates, as in our study.…”

Section: Discussionsupporting

confidence: 53%

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Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

et al. 2021

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Our study focused on investigating the possibilities of controlling the accumulation of carbohydrates in certain microalgae species (Arthrospira platensis Gomont, Chlorella vulgaris Beijer, and Dunaliella salina Teod) to determine their potential in biofuel production (biohydrogen). It was found that after the introduction of carbohydrates (0.05 g⋅L−1) into the nutrient medium, the growth rate of the microalgae biomass increased, and the accumulation of carbohydrates reached 41.1%, 47.9%, and 31.7% for Arthrospira platensis, Chlorella vulgaris, and Dunaliella salina, respectively. Chlorella vulgaris had the highest total carbohydrate content (a mixture of glucose, fructose, sucrose, and maltose, 16.97%) among the studied microalgae, while for Arthrospira platensis and Dunaliella salina, the accumulation of total carbohydrates was 9.59% and 8.68%, respectively. Thus, the introduction of carbohydrates into the nutrient medium can stimulate their accumulation in the microalgae biomass, an application of biofuel production (biohydrogen).

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“…In all the studies [ 36 , 37 , 38 , 39 , 40 , 41 ], stirring during the cultivation of microalgae was a factor that reduces their ability to produce carbohydrates, as in our study.…”

Section: Discussionsupporting

confidence: 53%

“…Liu et al [ 38 ] proposed a method for obtaining carbohydrates from Arthrospira platensis . In open industrial reservoirs, microalgae grew in conditions of nitrogen deficiency.…”

Section: Discussionmentioning

confidence: 99%

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

et al. 2021

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“…Liu et al [ 75 ] presented a method for producing a new type of polysaccharide from the microalga Arthrospira platensis . Microalgae were cultured under conditions of nitrogen deficiency in open industrial reservoirs.…”

Section: Microalgae Polysaccharidesmentioning

confidence: 99%

Microalgae: A Promising Source of Valuable Bioproducts

et al. 2020

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Microalgae are a group of autotrophic microorganisms that live in marine, freshwater and soil ecosystems and produce organic substances in the process of photosynthesis. Due to their high metabolic flexibility, adaptation to various cultivation conditions as well as the possibility of rapid growth, the number of studies on their use as a source of biologically valuable products is growing rapidly. Currently, integrated technologies for the cultivation of microalgae aiming to isolate various biologically active substances from biomass to increase the profitability of algae production are being sought. To implement this kind of development, the high productivity of industrial cultivation systems must be accompanied by the ability to control the biosynthesis of biologically valuable compounds in conditions of intensive culture growth. The review considers the main factors (temperature, pH, component composition, etc.) that affect the biomass growth process and the biologically active substance synthesis in microalgae. The advantages and disadvantages of existing cultivation methods are outlined. An analysis of various methods for the isolation and overproduction of the main biologically active substances of microalgae (proteins, lipids, polysaccharides, pigments and vitamins) is presented and new technologies and approaches aimed at using microalgae as promising ingredients in value-added products are considered.

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“…Hot water extraction of polysaccharides from terrestrial plant has been the most e cient, low cost and environmental-friendly method in a variety of applications [21,22]. One of the main purpose of this study is to remove potential heavy metal accumulation in this vegetable, thus careful use of edible chemical reagents were paid special attention through the puri cation steps.…”

Section: Discussionmentioning

confidence: 99%

Purification, Characterization, and Self-Assembly of the Polysaccharides from Allium Schoenoprasum

Zhang

Zheng

et al. 2020

Preprint

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Abstract Background: Allium schoenoprasum is a world-wide common vegetable while only its leave is used in the food factory. Its stalk is largely discarded, for potential heavy metal accumulations, which eventually lead to an environmental contamination. To fully utilize this vegetable and minimize its metal content, the major polysaccharide content is purified and characterized with chemical and computational approaches.Results: The major polysaccharide component from the stalk of Allium schoenoprasum (AssP) was extracted and purified. The gel filtration chromatography purified AssP exhibited a molecular weight of around 1.6 kDa, which was verified by MALDI-ToF-MS. Monosaccharide analysis revealed its composition as rhamnose: arabinose: galactose: glucose: mannose: fructose with a molar ratio of 0.0264:2.46:3.71:3.35:1.00:9.93, respectively. Multiple NMR analysis revealed its backbone as α-Ara/Glu/Gal-(1→2)-linked and β-D-Fru-(4→5)-linked sugar residues. There was no tertiary structure of this polysaccharide, however, it self-assembled into a homogenous nanoparticle with a diameter of ~600 nm. The solution behavior of this AssP polysaccharide was simulated, and there was no specific binding site on one molecule for another. Association of this polysaccharide was concentration dependent. As the AssP concentration increased, the spherical particles increased their sizes and eventually merged into cylindrical micelles. The diversity of AssP hydrodynamic behavior endowed potential versatility in its applications.Conclusions: AssP was characterized as a polysaccharide with identified monosaccharide compositions and linkage between them. Although there is no tertiary structure in one AssP molecule, self-assembly of AssP molecules could form nanoparticles or micelles depending on its solution concentrations. The unique AssP solution behavior endows itself a potential biomaterial for nanoparticles preparations.

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Production and structural characterization of a new type of polysaccharide from nitrogen-limited Arthrospira platensis cultivated in outdoor industrial-scale open raceway ponds

Cited by 40 publications

References 52 publications

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content

Microalgae: A Promising Source of Valuable Bioproducts

Purification, Characterization, and Self-Assembly of the Polysaccharides from Allium Schoenoprasum

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