Characterization of the polysaccharide from Cola millenii seeds

Abstract

We have isolated and characterized a water soluble polysaccharide from Cola millenii seeds. It was found to be composed of a total of 59% neutral sugars (mainly rhamnose, galactose and arabinose ~ 24, 13 and 8% respectively) and 41% uronic acids [mainly galacturonic acid]. The weight and number average molar mass values were found to be 4.7 x 10 6 g/mol and 3.5 x 10 6 g/mol, respectively. The polysaccharide exhibited polyelectrolyte properties with the intrinsic viscosity varying with salt concentration. The polysaccharide formed a highly viscous solution in water with apparent zero shear viscosities of 0.59 -772 Pa.s at concentrations 0.3 -2.5wt%. The solutions were shear thinning even at very low concentrations. The mechanical spectra showed gel-like characteristics at concentrations >2 wt%. The rheological behavior indicates the polysaccharide has potential for application as a thickener and suspending agent in food, pharmaceutical and cosmetic formulations.

Structural characterization of a non‐starch polysaccharide from sweet corn cobs

“…Then, the column was calibrated using the standard set of Dextran (MW 1300, 6000, 12,000, 22,000, 50,000, 110,000, 400,000, and 800,000). The molecular weight of SCP‐80‐I was measured using a standard curve (Nwokocha & Williams, 2020; Zheng et al, 2014).…”

Effects of Lactobacillus plantarum Fermentation on the Chemical Structure and Antioxidant Activity of Polysaccharides from Bulbs of Lanzhou Lily

“…The Lanzhou lily polysaccharides before and after fermentation were formulated into a solution with a concentration of 0.5 mg/mL, and the particle size distribution of the polysaccharide before and after fermentation was measured by a Nicomp 380 ZLS Zeta particle size analyzer based on dynamic light scattering (DLS) at 25 °C . An MCR301 Rheometer (Anton Paar, GmbH, Germany) was used to analyze the apparent viscosity of the lily polysaccharide solution (10 mg/mL) at 25 °C using a parallel plate clamp (P50 plate), a plate diameter of 50 mm, a rotor spacing of 1 mm, and a shear rate of 0.01 to 1000 s –1 …”